Coconut oil induced production of a surfactant-compatible lipase fromAspergillus tamariiunder submerged fermentation

Abstract

Filamentous fungi are efficient producers of lipases. The present study focuses on identification of a potent lipolytic fungus and enhancement of lipase production through optimization of nutritional and cultural conditions under submerged fermentation. Molecular characterization of the fungus by 18S rDNA sequencing revealed its identity as Aspergillus tamarii with 98% homology. Maximum lipase production was noted in mineral salts medium supplemented with coconut oil (2.5%, v/v). A combination of ammonium chloride (2%, w/v) and tryptone (2%, w/v) facilitated maximum lipase production at pH 5 of the production medium. A carbon: nitrogen ratio of 1:4 led to significant (p < 0.00008) increase in the enzyme production in the presence of surfactant cetyltrimethylammonium bromide (0.5%, w/v). Maximum lipase activity (2,32,500 ± 192 U/ml/min) was recorded after 7 days of incubation at 25 °C on a rotary shaker at 120 rpm. A 9.8-fold increase in lipase activity was recorded after optimization of the process parameters. Addition of crude lipase enhanced the oil stain removal activity of a commercially available detergent by 2.2-fold. The current findings suggest the potentiality of this fungal lipase to be used in detergent formulation.

Optimization of novel halophilic lipase production by Fusarium solani strain NFCCL 4084 using palm oil mill effluent

“…Therefore, in the present study, we aimed to estimate and establish the required concentrations of selected variables. A significantly high lipase activity of 3.93 (U/ml) recorded with POME could be due to the presence of high content of saturated fatty acids [16]. This is in accordance with the studies of Salihu et al [52], where POME upon addition to other production components was identified to greatly enhance the lipase production by Penicillium citrinum .…”

“…The variation in medium components as well as their concentrations has been found to influence fungal lipases production greatly [16], [25]. Therefore, in the present study, we aimed to estimate and establish the required concentrations of selected variables.…”

“…This repressive effect therefore, could be linked to increased mineral contents present in POME which are required in small quantities only [47]. Further, it could also be due to increased palm oil concentration which can interfere with the rate of oxygen diffusion and aeration affecting fungal growth negatively and in turn lipase production [16].…”

“…Owing to the ability to catalyze a wide range of reactions, lipases have attracted attention towards novel applications such as in the biodiesel production [5], detergents [10], food industries [11], pharmaceutical industry [12], cosmetics [13] and as biosensors [14]. Lipases are ubiquitous and are produced by plants, animals and microorganisms [15], [16], [17]. Among these sources, microbial lipases are widely preferred due to the high productivity efficiency and their ease of genetic manipulation [18].…”

Estudo da eficiência enzimática fúngica nas reações de transesterificação e hidrólise a partir de óleos vegetais nativos visando aplicações biotecnológicas

“…Ao analisar a literatura, vários autores reportam a produção de lipases por fungos em cultivo submerso suplementado com diferentes óleos. Lima et al (2003) Por fim, no estudo de Das et al (2016), com a finalidade de encontrar as melhores condições nutricionais e demais condições para a cultura de Aspergillus tamarii crescer e produzir lipase, foi observado a eficiência do óleo de coco para a fermentação submersa.…”