Cochleates bridged by drug molecules

Syed, Uwais M.; Woo, Amy F.; Plakogiannis, Fotios M.; Jin, Tao; Zhu, Hua

doi:10.1016/j.ijpharm.2008.06.026

Cited by 34 publications

(39 citation statements)

References 33 publications

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“…Other techniques have also been employed successfully in the identification and characterization of cochleates. Dynamic light scattering was used [50], mostly to determine cochleate particle size distribution [51]. Scanning electron microscopy has also been employed to visualize these structures [51].…”

Section: Methods To Identify and Characterize Cochleatesmentioning

confidence: 99%

“…Dynamic light scattering was used [50], mostly to determine cochleate particle size distribution [51]. Scanning electron microscopy has also been employed to visualize these structures [51]. Confocal fluorescence microscopy has been used to visualize binding and transfer of fluorescent lipids from cochleates to membranes and fusion with cell membranes [43,51].…”

Section: Methods To Identify and Characterize Cochleatesmentioning

confidence: 99%

“…Scanning electron microscopy has also been employed to visualize these structures [51]. Confocal fluorescence microscopy has been used to visualize binding and transfer of fluorescent lipids from cochleates to membranes and fusion with cell membranes [43,51]. Light microscopy shows different patterns depending on the molecule bridging liposomes [20].…”

Section: Methods To Identify and Characterize Cochleatesmentioning

confidence: 99%

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Lipid complexes with cationic peptides and OAKs; their role in antimicrobial action and in the delivery of antimicrobial agents

Epand

Mor

2011

Cell. Mol. Life Sci.

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Antimicrobial agents are toxic to bacteria by a variety of mechanisms. One mechanism that is very dependent on the lipid composition of the bacterial membrane is the clustering of anionic lipid by cationic antimicrobial agents. Certain species of oligo-acyl-lysine (OAK) antimicrobial agents are particularly effective in clustering anionic lipids in mixtures mimicking the composition of bacterial membranes. The clustering of anionic lipids by certain cationic antimicrobial agents contributes to the anti-bacterial action of these agents. Bacterial membrane lipids are a determining factor, resulting in some species of bacteria being more susceptible than others. In addition, lipids can be used to increase the effectiveness of antimicrobial agents when administered in vivo. Therefore, we review some of the structures in which lipid mixtures can assemble, to more effectively be utilized as antimicrobial delivery systems. We describe in more detail the complexes formed between mixtures of lipids mimicking bacterial membranes and an OAK and their usefulness in synergizing with antibiotics to overcome bacterial multidrug resistance.

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Section: Methods To Identify and Characterize Cochleatesmentioning

confidence: 99%

Section: Methods To Identify and Characterize Cochleatesmentioning

confidence: 99%

Section: Methods To Identify and Characterize Cochleatesmentioning

confidence: 99%

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Lipid complexes with cationic peptides and OAKs; their role in antimicrobial action and in the delivery of antimicrobial agents

Epand

Mor

2011

Cell. Mol. Life Sci.

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“…Large unilamellar vesicle (LUV)-type liposomes were obtained and suspended in 40% dextran solution. Liposomes were suspended in dextran and were added dropwise to a PEG 8000 solution (15%) under magnetic stirring for 1 h. 100 μM CaCl 2 solution was added dropwise and stirred for two more hours (13,14,(28)(29)(30). Finally, it was centrifuged at 3000 rpm for 30 min and supernatant was separated and raloxifene analyzed from the supernatant by HPLC for the calculation encapsulation efficiency.…”

Section: Preparation Of Cochleatesmentioning

confidence: 99%

The Effectiveness of Raloxifene-Loaded Liposomes and Cochleates in Breast Cancer Therapy

Ağardan

Değim

Yılmaz³

et al. 2015

AAPS PharmSciTech

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Abstract. Liposome (spherical vesicles) and cochleate (multilayer crystalline, spiral structure) formulations containing raloxifene have been developed having dimethyl-β-cyclodextrin (DM-β-CD) or sodium taurocholate (NaTC). Raloxifene was approved initially for the treatment of osteoporosis but it is also effective on breast tissue and endometrial cells. Raloxifene inhibits matrix metalloproteinase-2 (MMP-2) enzyme, which is known to be responsible for tumor invasion and the initiation of angiogenesis during the tumor growth. Therefore, raloxifene was selected as a model drug. A series of raloxifene-loaded liposome and cochleate formulations were prepared. In vitro release studies and in vivo tests were performed. Breast cancer cell lines (MCF-7) were also used to find the most effective formulation. Highest antitumor activity was observed, and MMP-2 enzyme was also found to be inhibited with raloxifene-loaded cochleates containing DM-β-CD. These developed formulations can be helpful for further treatment alternatives and new strategies for cancer therapy.

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“…Beside their usefulness as a system to deliver protein, peptide and DNA for vaccine and gene therapy applications (Mannino & Gould-Fogerite 1997, cochleates have also been used as a delivery system for a variety of drugs, including AmB (Santangelo et , Delmas et al 2002, Syed et al 2008, Livne et al 2010). Additionally, a comprehensive patent specifying that Leishmania major-infected mice were orally treated with AmB cochleates (CAMB) was recently published (Mannino 2010).…”

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confidence: 99%