Cochleate characterization by cryogenic electron microscopy methods: Cryo-TEM and Cryo-SEM

Hollander, Amit; Danino, Dganit

doi:10.1016/j.colsurfa.2015.07.025

Cited by 16 publications

(5 citation statements)

References 41 publications

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“…The developed self-assembled nanocochleates prepared by the hydrogel-based method showed a rodlike morphology while visualized under SEM (Figure A) and TEM (Figure A), which was similar to previously reported morphology of cochleate particles . Further, as shown in Figure C, the hydrodynamic diameter of three batches of nanocochleates was 385 ± 97 nm recorded using dynamic light scattering (DLS)-based measurements.…”

Section: Resultssupporting

confidence: 87%

Aerosol Delivery of Paclitaxel-Containing Self-Assembled Nanocochleates for Treating Pulmonary Metastasis: An Approach Supporting Pulmonary Mechanics

Shanmugam

Joshi

Kaviratna

et al. 2020

ACS Biomater. Sci. Eng.

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Paclitaxel (PTX) is a potent anticancer agent, which is clinically administered by infusion for treating pulmonary metastasis of different cancers. Systemic injection of PTX is promising in treating pulmonary metastasis of various cancers but simultaneously leads to many severe complications in the body. In this study, we have demonstrated a noninvasive approach for delivering PTX to deep pulmonary tissues via an inhalable phospholipid-based nanocochleate platform and showed its potential in treating pulmonary metastasis of melanoma cancer. Nanocochleates have been previously explored for oral delivery of anticancer drugs; their application for aerosol-based administration has not been accomplished in the literature thus far. Our results showed that the PTX-carrying aerosol nanocochleates (PTX-CPTs) possessed excellent pulmonary surfactant action characterized by high surface activity and encouraging in vitro terminal airway patency when compared to the marketed Taxol formulation, which is known to contain a high amount of Cremophore EL. We observed under in vitro twin-impinger analysis that the PTX-CPT had a high tendency to get deposited in stage II (alveolar region of lungs), indicating the capability of CPT to reach the deep alveolar region. Further, while exposed to the human lung adenocarcinoma cell line (A549), the PTX-CPT showed excellent cytotoxicity mediated by enhanced cellular uptake via energy-dependent endocytosis. Aerosol-based administration of PTX-CPT in a pulmonary metastatic murine melanoma model (B16F10) resulted in significant (p < 0.05) tumor growth inhibition when compared to an intravenous dose of Taxol. Inhibition of tumor growth in aerosol-based PTX-CPT-treated animals was evident by the significant (p < 0.05) reduction in numbers of tumor nodules and percent metastasis area covered by melanoma cells in the lung when compared to other treatment groups. Overall, our finding suggests that PTX can be safely administered in the form of an aerosol using a newly developed CPT system, which serves a dual purpose as both a drug delivery carrier and a pulmonary surfactant in treating pulmonary metastasis.

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Section: Resultssupporting

confidence: 87%

Aerosol Delivery of Paclitaxel-Containing Self-Assembled Nanocochleates for Treating Pulmonary Metastasis: An Approach Supporting Pulmonary Mechanics

Shanmugam

Joshi

Kaviratna

et al. 2020

ACS Biomater. Sci. Eng.

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“…Divalent cations (e.g., Ca 2+ , Mg 2+ ) can interact strongly with both zwitterionic and anionic lipids [50,51], but the interactions with anionic lipids (e.g., DMPS, DPPA) is rather remarkable [50], resulting in cochleation events governed by dehydration and chelation of head-group lipids [50,52]. In contrast, although monovalent cations (i.e., Na + ) penetrate deeply into a membrane and bind specifically to carbonyl oxygens of phospholipids, they slightly affect the physical properties of DOPC, even at high concentrations [53].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Calcium and protons affect the interaction of neurotransmitters and anesthetics with anionic lipid membranes

Pérez-Isidoro¹,

Ruiz‐Suárez²

2016

Biochimica et Biophysica Acta (BBA) - Biomembranes

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We study how zwitterionic and anionic biomembrane models interact with neurotransmitters (NTs) and anesthetics (ATs) in the presence of Ca(2+) and different pH conditions. As NTs we used acetylcholine (ACh), γ-aminobutyric acid (GABA), and l-glutamic acid (LGlu). As ATs, tetracaine (TC), and pentobarbital (PB) were employed. By using differential scanning calorimetry (DSC), we analyzed the changes such molecules produce in the thermal properties of the membranes. We found that calcium and pH play important roles in the interactions of NTs and ATs with the anionic lipid membranes. Changes in pH promote deprotonation of the phosphate groups in anionic phospholipids inducing electrostatic interactions between them and NTs; but if Ca(2+) ions are in the system, these act as bridges. Such interactions impact the physical properties of the membranes in a similar manner that anesthetics do. Beyond the usual biochemical approach, we claim that these effects should be taken into account to understand the excitatory-inhibitory orchestrated balance in the nervous system.

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“…The technique allows analysing the samples at their native hydrated state of the samples at high resolution, nano to the micro-scale. For instance, Hollander and Danino used Cryo-TEM to investigate the structures of the cochleates made of dioleoylphosphatidylserine (DOPS) that are negatively charged lipid microstructures and calcium, which induce the binding of lipid layers as well as complex formation [62] (Figure 3.29). Three-dimensional transmission electron microscope ( 3D-TEM): 3D images can be obtained with proper detectors and software [55].…”

Section: Electron Energy Loss Spectroscopy ( Eels)mentioning

confidence: 99%

Surface Characterization Techniques

Erdogan

Güler

Kilic

et al. 2016

Surface Treatments for Biological, Chemical, and Physical Applications

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The chemical, physical, and morphological features of surfaces play a very crucial role in material properties including wettability, optical, adhesive, mechanical, and so on. Therefore, diagnosis of surface structures is very important for materials science. The properties of natural and fabricated material surfaces can be determined using characterization techniques. In this chapter, some of the most common characterization methods are given. Surface Characterization Methods X-ray Spectroscopy TechniquesThe discovery of X-rays in 1845 by Wilhelm Conrad Rontgen gave rise to significant scientific advancement that benefited a variety of fields by making the invisible to the visible. In 1901, Wilhelm Rontgen received the Nobel Prize in physics for the invention of X-rays. The discovery of X-ray led to significant improvement in the diagnosis for medical applications. In the years subsequent to this discovery, X-rays were used for military applications. Initially, the harmful effects of radiation were not comprehended. Later, it was observed that those who were exposed to X-ray radiation encountered severe burns and skin damages. Thomas Edison's assistant who worked extensively with X-rays, died of skin cancer in 1904. Then, the risk of X-ray radiation was fully understood and this led to the use of protection shields. Today, X-ray technology is being widely employed in numerous fields such as medicine, material analysis, and devices [1].X-rays are electromagnetic energy waves acting like light rays. X-rays have wavelengths in the angstrom range. Furthermore, X-rays are highly energetic and are employed not only to penetrate solids but also to probe their internal structure. As a result of the atom excitation via high energy photons or electrons, an electron is removed from the atom, leading to an electron hole at the inner orbit. These electron holes are filled by outer electrons. As a result

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Cochleate characterization by cryogenic electron microscopy methods: Cryo-TEM and Cryo-SEM

Cited by 16 publications

References 41 publications

Aerosol Delivery of Paclitaxel-Containing Self-Assembled Nanocochleates for Treating Pulmonary Metastasis: An Approach Supporting Pulmonary Mechanics

Aerosol Delivery of Paclitaxel-Containing Self-Assembled Nanocochleates for Treating Pulmonary Metastasis: An Approach Supporting Pulmonary Mechanics

Calcium and protons affect the interaction of neurotransmitters and anesthetics with anionic lipid membranes

Surface Characterization Techniques

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