Cocaine- and amphetamine-regulated transcript and calcium binding proteins immunoreactivity in the subicular complex of the guinea pig

Ontogeny of Neurons Containing Calcium‐Binding Proteins in the Preoptic Area of the Guinea Pig: Sexually Dimorphic Development of Calbindin

“…In order to show the binding sites of the primary antisera with antigens, sections were then incubated (1 h, at room temperature) with a mixture of anti‐rabbit Alexa Fluor 555 and anti‐mouse Alexa Fluor 488 secondary antibodies (Table ). The method details have been described in our earlier manuscripts (Wasilewska et al ., ; Hermanowicz‐Sobieraj and Robak, ).…”

“…Moreover, these proteins may have an impact on growth cone motility, neurite sprouting, elongation and branching as well as on neuronal cell proliferation, programmed cell death and gene expression (Mattson et al ., ; Mattson, ; Stuart and Lephart, ; Fields et al ., ). Even though the exact physiological function of CB, CR, and PV is still not completely understood, they have been demonstrated to be important markers of separate neuronal populations in the adult brain (Arai et al ., ; Baimbridge et al ., ; Andressen et al ., ; Najdzion et al ., ; Równiak et al ., ; Wasilewska et al ., ; Hermanowicz‐Sobieraj et al ., ) and during brain ontogeny (Solbach and Celio, ; Zakowski and Robak, 2013; Hermanowicz‐Sobieraj and Robak, ). In the guinea pig, these proteins mark the vast majority of GABAergic neurons in amygdala, septum, and subicular complex (Równiak et al ., ; Wasilewska et al ., ; Hermanowicz‐Sobieraj and Robak, ; Równiak, ).…”

“…Even though the exact physiological function of CB, CR, and PV is still not completely understood, they have been demonstrated to be important markers of separate neuronal populations in the adult brain (Arai et al ., ; Baimbridge et al ., ; Andressen et al ., ; Najdzion et al ., ; Równiak et al ., ; Wasilewska et al ., ; Hermanowicz‐Sobieraj et al ., ) and during brain ontogeny (Solbach and Celio, ; Zakowski and Robak, 2013; Hermanowicz‐Sobieraj and Robak, ). In the guinea pig, these proteins mark the vast majority of GABAergic neurons in amygdala, septum, and subicular complex (Równiak et al ., ; Wasilewska et al ., ; Hermanowicz‐Sobieraj and Robak, ; Równiak, ). In the amygdala neurons expressing CB and/or PV are sexually dimorphic and PV+ and CB+ neurons co‐express estrogen receptors beta (ERβ), while CR+ neurons co‐express estrogen receptors alpha (ERα) (Równiak et al ., ; Równiak, ).…”

GABAergic and Glutamatergic Phenotypes of Neurons Expressing Calcium-Binding Proteins in the Preoptic Area of the Guinea Pig

“…As some glutamic acid decarboxylase (GAD) neurons may also contain PAG, some BF neurons may have the capacity to synthesize GABA and glutamate [ 12 ]. In the guinea pig, CaBPs have been used as important markers for distinguishing subpopulations of neurons and pathways in adult and developing brains [ 13 , 14 , 15 ]. For example, these proteins mark the vast majority of GABAergic neurons in the guinea pig amygdala and subicular complex [ 4 , 14 ].…”

“…In the guinea pig, CaBPs have been used as important markers for distinguishing subpopulations of neurons and pathways in adult and developing brains [ 13 , 14 , 15 ]. For example, these proteins mark the vast majority of GABAergic neurons in the guinea pig amygdala and subicular complex [ 4 , 14 ]. CR functions as a marker of immature neurons during guinea pig brain development, and it may be used for defining fetal stages in the septum and anterior thalamic nuclei [ 16 , 17 ], whereas CB and PV function as markers of postnatal stages during development of the guinea pig anterior thalamic and septum, respectively [ 16 , 17 ].…”