Ontogeny of Neurons Containing Calcium‐Binding Proteins in the Preoptic Area of the Guinea Pig: Sexually Dimorphic Development of Calbindin

Bogus‐Nowakowska, Krystyna

doi:10.1002/dneu.22662

Cited by 4 publications

(12 citation statements)

References 122 publications

(203 reference statements)

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“…This may be due to the very heterogeneous matrix morphology during early morphogenesis, in which the cytoarchitecture and the chemoarchitecture of the hypothalamus are still largely undifferentiated. In this sense, as suggested by Bogus‐Nowakowska in guinea pig brain, the elevated levels of CR, CB, and later PV could be linked at subsequent maturation, differentiation, and synaptogenesis process (Bogus‐Nowakowska, ).…”

Section: Discussionmentioning

confidence: 87%

“…Despite there are only a few data in the literature on earlier gestation, an ontogenetic study performed to investigate CBPs in the preoptic are of guinea pig (gestation period 58–70 days) showed elevated levels at embryonic day 40 of CB and CR immunoreactivity with a peak at embryonic day 50. Contrary the PV appeared later and peaked at postnatal day 10 (Bogus‐Nowakowska, ).…”

Section: Discussionmentioning

confidence: 99%

“…The topography of the hypothalamic region and the anatomical characterization of the structures were determined by comparison with detailed atlases relative to human (Bayer & Altman, 2007, 2019, mouse (Sunkin et al, 2012), and Lama glama (Welker, Johnson, Noe, n.d.).…”

Section: Nissl Staining and Topographic Mappingmentioning

confidence: 99%

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Neurochemical characterization of the hypothalamus of the early fetal and newborn alpaca Vicugna pacos

Montelli

Graïc

Ruiz

et al. 2019

The Anatomical Record

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In this study we performed a neurochemical characterization of the hypothalamus in the developing alpaca (Vicugna pacos) with the aim of revealing the distributions of immunoreactive (-ir) cells containing parvalbumin (PV), calbindin (CB), calretinin (CR), the somatostatin (SOM), the enzyme aromatase P450 (P450Arom), the estrogen receptor α (ER-α), and estrogen receptor β (ER-β) in embryonal stages, early fetal age, and in the newborn. This analysis has been carried out on embryos at 20, 30, 45 days, fetuses at 90 days, and newborn alpaca. Our immunohistochemical results revealed no cells-ir throughout the embryonic hypothalami of 20, 30, and 45 days. On the fetal stage of 90 days, SOM-ir cells were observed in the lateral hypothalamus and the ventromedial nuclei of the tuberal region. We checked for the presence of P450Arom-ir cells in the periventricular area and dorsomedial hypothalamic nucleus of the tuberal region. In these fetal stages, no PV-ir, CB-ir, CR-ir or ERs-ir cells were identified. In the newborn, the PV-ir, CB-ir, CR-ir, and SOMir cells were detected in both the anterior and tuberal hypothalamic area. The P450Arom-ir cells the ER-α-ir and ER-β-ir cells were found in the anterior hypothalamus. Our results offer a contribution in the future purpose to obtain a time-expression pattern of the considered markers in alpaca during gestation and represents a foundation for future investigations on the alpaca brain to define the cross talk between PV, CB, CR, P450Arom, SOM, and ERs in the hypothalamus, the strategic region for the control of the reproductive behavior.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

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Neurochemical characterization of the hypothalamus of the early fetal and newborn alpaca Vicugna pacos

Montelli

Graïc

Ruiz

et al. 2019

The Anatomical Record

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“…CB expression is sexually dimorphic in the preoptic area and anterior hypothalamus of rodents. In the preoptic area, CB immunoreactivity was several times higher in males compared to females (Bogus‐Nowakowska, 2019; Edelmann, Wolfe, Scordalakes, Rissman, & Tobet, 2007; Sickel & McCarthy, 2000; Yamaguchi, Abe, Maejima, & Tsukahara, 2018). However, there were no apparent differences in the amount of CB‐IR neurons in the mouse VMH (Edelmann et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…CB and CR are found in separate groups of neurons in the central and peripheral nervous systems (Hof et al, 1999; Masliukov, Nozdrachev, & Emanuilov, 2016; Schmidt, 2012; Schwaller, 2014). In the hypothalamus, CB and CR are observed in the medial preoptic area, paraventricular, suprachiasmatic, supraoptic, infundibular, arcuate, ventromedial, dorsomedial nucleus, lateral hypothalamus and the mammillary bodies (Belekhova, Kenigfest, Chernigovskaya, & Veselkin, 2017; Bogus‐Nowakowska, 2019; Celio, 1990; Eghlidi et al, 2018; Foo, Hellysaz, & Broberger, 2014; Fortin & Parent, 1997; Montelli, Graïc, Ruiz, Stelletta, & Peruffo, 2020).…”

Section: Introductionmentioning

confidence: 99%

Expression of calbindin and calretinin in the dorsomedial and ventromedial hypothalamic nuclei during aging

Vishnyakova

Moiseev

Spirichev

et al. 2020

The Anatomical Record

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The hypothalamus is involved in the regulation of rhythms, autonomic, endocrine, and behavioral functions and may also participate in aging development and control. The aim of this work was to study the expression of calbindin (CB) and calretinin (CR) in the ventromedial (VMH) and dorsomedial (DMH) hypothalamic nuclei in young and old rats of both sexes by immunohistochemistry and western blotting. In young animals, the largest number of CB‐immunoreactive (IR) neurons was detected in the ventral part of DMH (DMHv) and smaller percentage was found in its dorsal part (DMHd), in the dorsomedial part of the VMH (VMHdm) and in the ventrolateral part of the VMH (VMHvl). In aged animals, the percentage of CB‐IR neurons significantly decreased in all studied nuclei, including DMHv, DMHd, VMHdm and VMHvl. CR‐IR neurons were found in moderate number in the DMHv, DMHd, VMHdm and VMHvl of young rats. In aged rats, the percentage of CR‐IR neurons significantly increased in the DMHv, DMHd, VMHdm and VMHvl. Less than one third of IR neurons colocalized CB and CR in young and aged rats. The expression of CB significantly decreased, and the expression of CR significantly increased in the DMH and VMH during aging by western blot analysis. Thus, there are opposite changes of the calcium‐binding proteins expression in the hypothalamic nuclei involved in the metabolic and sexual regulation during aging.

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Two-Step Actions of Testicular Androgens in the Organization of a Male-Specific Neural Pathway from the Medial Preoptic Area to the Ventral Tegmental Area for Modulating Sexually Motivated Behavior

Morishita,

Kobayashi,

Mitsuzuka

et al. 2023

J. Neurosci.

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The medial preoptic area (MPOA) is a sexually dimorphic region of the brain that regulates social behaviors. The sexually dimorphic nucleus (SDN) of the MPOA has been studied to understand sexual dimorphism, although the anatomy and physiology of the SDN is not fully understood. Here, we characterized SDN neurons that contribute to sexual dimorphism and investigated the mechanisms underlying the emergence of such neurons and their roles in social behaviors. A target-specific neuroanatomical study using transgenic mice expressing Cre recombinase under the control ofCalb1, a gene expressed abundantly in the SDN, revealed that SDN neurons are divided into two subpopulations: GABA neurons projecting to the ventral tegmental area (VTA), where they link to the dopamine system (CalbVTAneurons) and GABA neurons that extend axons in the MPOA or project to neighboring regions (CalbnonVTAneurons). CalbVTAneurons were abundant in males, but were scarce or absent in females. There was no difference in the number of CalbnonVTAneurons between sexes. Additionally, we found that emergence of CalbVTAneurons requires two testicular androgen actions that occur first in the postnatal period and second in the peripubertal period. Chemogenetic analyses of CalbVTAneurons indicated a role in modulating sexual motivation in males. Knockdown ofCalb1in the MPOA reduced the intromission required for males to complete copulation. These findings provide strong evidence that a male-specific neural pathway from the MPOA to the VTA is organized by the two-step actions of testicular androgens for the modulation of sexually motivated behavior.Significance StatementThe medial preoptic area (MPOA) is a sexually dimorphic region of the brain that regulates social behaviors, although its sexual dimorphism is not fully understood. Here, we describe a population of MPOA neurons that contribute to the sexual dimorphism. These neurons only exist in masculinized brains, and they project their axons to the ventral tegmental area, where they link to the dopamine system. Emergence of such neurons requires two testicular androgen actions that occur first in the postnatal period and second in the peripubertal period. These MPOA neurons endow masculinized brains with a neural pathway from the MPOA to the ventral tegmental area and modulate sexually motivated behavior in males.

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Ontogeny of Neurons Containing Calcium‐Binding Proteins in the Preoptic Area of the Guinea Pig: Sexually Dimorphic Development of Calbindin

Cited by 4 publications

References 122 publications

Neurochemical characterization of the hypothalamus of the early fetal and newborn alpaca Vicugna pacos

Neurochemical characterization of the hypothalamus of the early fetal and newborn alpaca Vicugna pacos

Expression of calbindin and calretinin in the dorsomedial and ventromedial hypothalamic nuclei during aging

Two-Step Actions of Testicular Androgens in the Organization of a Male-Specific Neural Pathway from the Medial Preoptic Area to the Ventral Tegmental Area for Modulating Sexually Motivated Behavior

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