Cobblestone area-forming cells, long-term culture-initiating cells and NOD/SCID repopulating cells in human neonatal blood: a comparison with umbilical cord blood

Abstract

Summary:Our prior study demonstrated that neonatal blood (NB) contained hematopoietic stem and progenitor cells that declined rapidly after birth. To validate that NB is a source of functional stem cells, we characterized this population in terms of cobblestone area-forming cells (

Improvement of Postnatal Neovascularization by Human Adipose Tissue-Derived Stem Cells

“…Indeed, the cells exhibited a high proliferative capacity and an ability to change their morphology depending on the culture medium, and more specifically, to spontaneously form cobblestone area, a property attributed to macrocirculation-derived ECs and stem cells. 18 Further characterization showed that the CD34 ϩ /CD31 Ϫ cells lacked the hematopoietic lineage markers CD45 and CD14. 19,20 Moreover, because 20% of the CD34 ϩ /CD31 Ϫ cells also expressed the primitive cell marker ABCG2, it is suggested that the CD34 ϩ /CD31 Ϫ cell population contains a stem cell subset not already committed into a specific lineage.…”

Cobblestone-Area Forming Cells Derived from Patients with Mantle Cell Lymphoma Are Enriched for CD133+ Tumor-Initiating Cells

“…More specifically, during long-term co-culture we observed clusters of phase-dark cells beneath the stromal layer. These clusters are morphologically similar to CAFC that form under stromal cell monolayer when normal HSC are co-cultured with BM stromal cells [25], [44], [46]. In this study, immunophenotypic characterization of MCL associated CAFCs reveal that these clusters are composed of cells with a unique and primitive phenotype (CD45+CD19−CD133+) compared to cells that remain in suspension and the bulk population (CD45+CD19+CD133−).…”

“…To gain further insight into the existence of MCL-IC we utilized an ex vivo co-culture similar to the previously developed CAFC assay used to study normal HSC and progenitor cells [23], [25], [44]–[47]. Using this system we demonstrate for the first time that human mesenchymal stromal or the murine BM stromal cell line MS-5 can be used to prospectively identify and expand a unique subpopulation of primary MCL.…”

Novel alginate three‐dimensional static and rotating culture systems for effective ex vivo amplification of human cord blood hematopoietic stem cells and in vivo functional analysis of amplified cells in NOD/SCID mice

“…The cells collected from mouse marrow or spleen were first cultured in a 100‐mm dish for 4 to 8 hours to remove the adherent murine stromal cells to minimize the growth of mouse‐origin CFCs . The suspension cells were inoculated in the same human‐specific CFC culture as previously mentioned (“Colony‐forming cell assay”) at 1 × 10 5 to 2 × 10 5 cells per dish and grown for 14 days in a 37°C incubator with 5% CO 2 .…”