Novel alginate three‐dimensional static and rotating culture systems for effective ex vivo amplification of human cord blood hematopoietic stem cells and in vivo functional analysis of amplified cells in <scp>NOD</scp>/<scp>SCID</scp> mice

Yuan, Yan; Sin, Wai-Yee; Xue, Bofu; Yan, Ke; Tse, Kai-Tai; Chen, Zi; Xie, Yun; Xie, Yong

doi:10.1111/trf.12103

Cited by 8 publications

(8 citation statements)

References 30 publications

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“…This diffusion limitation of cells is probably determined by the concentration or stiffness of the alginate gel scaffold [ 26 , 27 ]. In fact, nonetheless, the cells grew well in the gel porous scaffold, which indicates that the gel has a good permeability, and the nutrients such as oxygen and cytokines in the liquid can straightway diffuse into cells through the gel system [ 28 ]. As well, the pore size of gel at the concentration of 100 mM is enough for cells growth, combined with lots of little channels between the adjacent pores [ 21 ], which would not result in local regions of hypoxia or metabolite limitations.…”

Section: Discussionmentioning

confidence: 99%

Wound Dressing Model of Human Umbilical Cord Mesenchymal Stem Cells‐Alginates Complex Promotes Skin Wound Healing by Paracrine Signaling

Wang

Yang

Tang

et al. 2015

Stem Cells International

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Purpose. To probe growth characteristics of human umbilical cord mesenchymal stem cells (hUCMSCs) cultured with alginate gel scaffolds, and to explore feasibility of wound dressing model of hUCMSCs-alginates compound. Methods. hUCMSCs were isolated, cultured, and identified in vitro. Then cells were cultivated in 100 mM calcium alginate gel, and the capacity of proliferation and migration and the expression of vascular endothelial growth factors (VEGF) were investigated regularly. Wound dressing model of hUCMSCs-alginate gel mix was transplanted into Balb/c mice skin defects. Wound healing rate and immunohistochemistry were examined. Results. hUCMSCs grew well but with little migration ability in the alginate gel. Compared with control group, a significantly larger cell number and more VEGF expression were shown in the gel group after culturing for 3–6 days (P < 0.05). In addition, a faster skin wound healing rate with more neovascularization was observed in the hUCMSCs-alginate gel group than in control groups at 15th day after surgery (P < 0.05). Conclusion. hUCMSCs can proliferate well and express massive VEGF in calcium alginate gel porous scaffolds. Wound dressing model of hUCMSCs-alginate gel mix can promote wound healing through paracrine signaling.

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Section: Discussionmentioning

confidence: 99%

Wound Dressing Model of Human Umbilical Cord Mesenchymal Stem Cells‐Alginates Complex Promotes Skin Wound Healing by Paracrine Signaling

Wang

Yang

Tang

et al. 2015

Stem Cells International

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“…Alginate, due to its capability to jellify under mild conditions and to allow easy retrieval of encapsulated cells, has been used to encapsulate and amplify HSPC from UCB either under static or dynamic conditions . This allows alginate‐encapsulated HSPC to be inserted in a high cellular density microenvironment, thus being more easily accessible to paracrine signaling .…”

Section: ‐D Co‐culture Of Hspc and Mscmentioning

confidence: 99%

Hematopoietic Niche – Exploring Biomimetic Cues to Improve the Functionality of Hematopoietic Stem/Progenitor Cells

Costa

Soure

Cabral

et al. 2017

Biotechnology Journal

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The adult bone marrow (BM) niche is a complex entity where a homeostatic hematopoietic system is maintained through a dynamic crosstalk between different cellular and non-cellular players. Signaling mechanisms triggered by cell-cell, cell-extracellular matrix (ECM), cell-cytokine interactions, and local microenvironment parameters are involved in controlling quiescence, self-renewal, differentiation, and migration of hematopoietic stem/progenitor cells (HSPC). A promising strategy to more efficiently expand HSPC numbers and tune their properties ex vivo is to mimic the hematopoietic niche through integration of adjuvant stromal cells, soluble cues, and/or biomaterial-based approaches in HSPC culture systems. Particularly, mesenchymal stem/stromal cells (MSC), through their paracrine activity or direct contact with HSPC, are thought to be a relevant niche player, positioning HSPC-MSC co-culture as a valuable platform to support the ex vivo expansion of hematopoietic progenitors. To improve the clinical outcome of hematopoietic cell transplantation (HCT), namely when the available HSPC are present in a limited number such is the case of HSPC collected from umbilical cord blood (UCB), ex vivo expansion of HSPC is required without eliminating the long-term repopulating capacity of more primitive HSC. Here, we will focus on depicting the characteristics of co-culture systems, as well as other bioengineering approaches to improve the functionality of HSPC ex vivo.

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“…When developing an in vitro HSPC model, it is of importance to optimize both the cellular compartment of the model, as well as the used hydrogel mimicking the ECM of the niche. To mimic the endosteal niche, hydrogels (e.g., Matrigel, collagen, Puramatrix, alginate, or PEG) or bone mimicking materials (e.g., βTCP, hydroxyapatite, or bioderived bone) have been used. In addition to these materials, models used either stromal cell lines or human primary cells as supportive cell sources.…”

Section: Introductionmentioning

confidence: 99%

A Human Hematopoietic Niche Model Supporting Hematopoietic Stem and Progenitor Cells In Vitro

Braham

Yim

Mateos

et al. 2019

Adv Healthcare Materials

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Niches in the bone marrow regulate hematopoietic stem and progenitor cell (HSPC) fate and behavior through cell–cell interactions and soluble factor secretion. The niche‐HSPC crosstalk is a very complex process not completely elucidated yet. To aid further investigation of this crosstalk, a functional in vitro 3D model that closely represents the main supportive compartments of the bone marrow is developed. Different combinations of human stromal cells and hydrogels are tested for their potential to maintain CD34+ HSPCs. Cell viability, clonogenic hematopoietic potential, and surface marker expression are assessed over time. Optimal HSPC support is obtained in presence of adipogenic and osteogenic cells, together with progenitor derived endothelial cells. When cultured in a bioactive hydrogel, the supportive cells self‐assemble into a hypoxic stromal network, stimulating CD34+CD38+ cell formation, while maintaining the pool of CD34+38− HSPCs. HSPC clusters colocalize with the stromal networks, in close proximity to sinusoidal clusters of CD31+ endothelial cells. Importantly, the primary in vitro niche model supports HSPCs with no cytokine addition. Overall, the engineered primary 3D bone marrow environment provides an easy and reliable model to further investigate interactions between HSPCs and their endosteal and perivascular niches, in the context of normal hematopoiesis or blood‐related diseases.

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Novel alginate three‐dimensional static and rotating culture systems for effective ex vivo amplification of human cord blood hematopoietic stem cells and in vivo functional analysis of amplified cells in NOD/SCID mice

Cited by 8 publications

References 30 publications

Wound Dressing Model of Human Umbilical Cord Mesenchymal Stem Cells‐Alginates Complex Promotes Skin Wound Healing by Paracrine Signaling

Wound Dressing Model of Human Umbilical Cord Mesenchymal Stem Cells‐Alginates Complex Promotes Skin Wound Healing by Paracrine Signaling

Hematopoietic Niche – Exploring Biomimetic Cues to Improve the Functionality of Hematopoietic Stem/Progenitor Cells

A Human Hematopoietic Niche Model Supporting Hematopoietic Stem and Progenitor Cells In Vitro

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