Coaxial electrospun aligned tussah silk fibroin nanostructured fiber scaffolds embedded with hydroxyapatite–tussah silk fibroin nanoparticles for bone tissue engineering

Shao, Weili; He, Jianxin; Feng, Suying; Ding, Bin; Chen, Li; Cui, Shizhong; Li, Kejing; Han, Qiming; Tan, Weilin

doi:10.1016/j.msec.2015.08.046

Cited by 146 publications

(64 citation statements)

References 35 publications

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“…Shao et al [120] showed that these nanostructured constructions have excellent biomimetic and mechanical properties, maintain the adhesion and proliferation of cells, while functionally contributing to biomineralization. The fibroin biomimetic matrices with added hydroxyapatite contain three reviews layers: one is of cartilage with longitudinally oriented microtubules; another is a bone layer with a 3D porous structure, and the intermediate layer has a dense structure that can effectively maintain the regeneration of cartilage and bone tissue in vivo [121].…”

Section: Use Of Silk Fibroin and Spidroin In Tissue Engineering And Rmentioning

confidence: 99%

Silk Fibroin and Spidroin Bioengineering Constructions for Regenerative Medicine and Tissue Engineering (Review)

Agapova¹

2017

Sovrem Tehnol Med

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The review is about the developments of modern bioengineering constructs from two unique biopolymers: the main protein of silkworm silk, fibroin, and frame silk of spider web, spidroin, and their applications in regenerative medicine and tissue engineering. Both types of polymers possess such important properties as biocompatibility, biodegradability, high strength and elasticity. Availability of silkworm cocoons in nature, debugged methods of fibroin purification make this protein very perspective in bioengineering constructs. A spider web protein, spidroin, is less common in nature, but the development of alternative methods for its production makes it a promising biopolymer.The structure and properties of silk fibroin and spidroin, their advantages over other natural and synthetic polymers, technologies of biopolymer construct fabrication are considered in this review. Silk fibroin and spidroin are shown to be applied for creation of 3D matrices promoting regeneration of damaged organs and tissues, biodegradable cell carriers and pharmaceutical preparations.

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Section: Use Of Silk Fibroin and Spidroin In Tissue Engineering And Rmentioning

confidence: 99%

Silk Fibroin and Spidroin Bioengineering Constructions for Regenerative Medicine and Tissue Engineering (Review)

Agapova¹

2017

Sovrem Tehnol Med

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“…The compositions of natural bone serve as an inspiration to design a novel organic-inorganic hybrid bone substitute [12]. Chitosan (CH) is a positively charged natural polymer that is obtained by partial deacetylation (with enzymatic or alkaline treatment) of chitin [13].…”

Section: Introductionmentioning

confidence: 99%

Calcium Silicate/Chitosan-Coated Electrospun Poly (Lactic Acid) Fibers for Bone Tissue Engineering

Su¹,

Wei

et al. 2017

Materials

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Electrospinning technology allows fabrication of nano- or microfibrous fibers with inorganic and organic matrix and it is widely applied in bone tissue engineering as it allows precise control over the shapes and structures of the fibers. Natural bone has an ordered composition of organic fibers with dispersion of inorganic apatite among them. In this study, poly (lactic acid) (PLA) mats were fabricated with electrospinning and coated with chitosan (CH)/calcium silicate (CS) mixer. The microstructure, chemical component, and contact angle of CS/CH-PLA composites were analyzed by scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. In vitro, various CS/CH-coated PLA mats increased the formation of hydroxyapatite on the specimens’ surface when soaked in cell cultured medium. During culture, several biological characteristics of the human mesenchymal stem cells (hMSCs) cultured on CS/CH-PLA groups were promoted as compared to those on pure PLA mat. Increased secretion levels of Collagen I and fibronectin were observed in calcium silicate-powder content. Furthermore, with comparison to PLA mats without CS/CH, CS10 and CS15 mats markedly enhanced the proliferation of hMSCs and their osteogenesis properties, which was characterized by osteogenic-related gene expression. These results clearly demonstrated that the biodegradable and electroactive CS/CH-PLA composite mats are an ideal and suitable candidate for bone tissue engineering.

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“…Thus, the components of natural bones inspire us to design novel organic-inorganic hybrid bone substitute [13]. Chitosan (CH) is a natural polymer with positively charged that is a derivative of chitin and is obtained by the partial deacetylation of chitin with alkaline or enzymatic hydrolysis [14].…”

Section: Introductionmentioning

confidence: 99%

Calcium Silicate/Chitosan-Coated Electrospun Poly (lactic acid) Fibers for Bone Tissue Engineering

Su¹,

Wei

et al. 2017

Preprint

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Electrospinning is the versatile technique to generate large quantities of micro-or nanofibers from a wide variety of shapes and sizes of polymer. Natural bone is a hierarchically composites with the dispersion of inorganic ceramic along organic polymer. The aim of this study, the electrospun poly (lactic acid) (PLA) mats coated with chitosan (CH) and calcium silicate (CS) powder were fabricated. The morphology, chemical composition, and surface properties of CS/CH-PLA composites were characterized by scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. In vitro, the CS/CH-coated PLA mats increased the formation of apatite on the surface when soaking in cell cultured medium. During culture, the adhesion and proliferation of the human mesenchymal stem cells (hMSCs) cultured on CS/CH-PLA were significantly promoted relative to those on PLA. Collagen I and fibronectin levels and promoted cell adhesion were observed upon an increase in CS content. Further, compared to PLA mats without CS/CH, CS10 and CS15 mats markedly enhanced the proliferation of hMSCs as well as their osteogenesis properties, which was characterized by bone-related gene expression. Our results demonstrated that the biodegradable and electroactive CS/CH-PLA mats had potential application as an ideal candidate for bone tissue engineering.Together, findings from this study clearly demonstrated that PLLA-C2S composite scaffold may function as an ideal candidate for bone tissue engineering.

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Coaxial electrospun aligned tussah silk fibroin nanostructured fiber scaffolds embedded with hydroxyapatite–tussah silk fibroin nanoparticles for bone tissue engineering

Cited by 146 publications

References 35 publications

Silk Fibroin and Spidroin Bioengineering Constructions for Regenerative Medicine and Tissue Engineering (Review)

Silk Fibroin and Spidroin Bioengineering Constructions for Regenerative Medicine and Tissue Engineering (Review)

Calcium Silicate/Chitosan-Coated Electrospun Poly (Lactic Acid) Fibers for Bone Tissue Engineering

Calcium Silicate/Chitosan-Coated Electrospun Poly (lactic acid) Fibers for Bone Tissue Engineering

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