Coat protein sequence comparisons of three Mexican isolates of papaya ringspot virus with other geographical isolates reveal a close relationship to American and Australian isolates

Abstract

The coat protein gene of the papaya ringspot virus was cloned and sequenced in three Mexican isolates (two from Veracruz, and one from Chiapas). The sequences of these viral isolates were compared to those of eleven isolates from other parts of the world. They had higher similarity to isolates from Australia and the United States than to Asian isolates. A region of about one hundred nucleotides neighboring the putative aphid transmission triplet of the coat protein, contained repeats of an EK (glutamic acid-lysine) motif in all the sequences. The bearing of this region on the genetic relationships and geographical distribution of the isolates is analyzed and discussed.

Molecular characterization of a severe isolate of papaya ringspot virus in Mexico and its relationship with other isolates

“…It was detected in Mexico in 1975 as the cause of severe damages in the main producing papaya states [3]. Previous studies of genetic diversity in the genome of PRSV from diverse world origins have focused on specific cistrons such as the one coding for the coat protein CP [4][5][6][7]. For instance, in an earlier study of sequence variation of the CP cistron and its product in diverse Mexican isolates, we concluded that there was a clear correlation between sequence variation and the geographical origins of the virus isolates [8] and that this diversity paralleled that found in similar studies [4,5].…”

Etiologia e estratégias de controle de viroses do mamoeiro no brasil

“…Visando encontrar uma alternativa de controle para o PRSV-P em pomares de mamoeiro nos municípios de Colima, Veracruz e Guerrero, no México, onde o PRSV-P causa perdas expressivas de até 85% na produtividade (Silva-Rosales et al, 2000), através de plantas transgênicas operando pelo princípio do CPGMR, foi realizada a caracterização do gene cp de três isolados mexicanos. Quando comparadas conjuntamente com as de sete isolados asiáticos, as seqüências de genes cp dos isolados mexicanos de PRSV-P estudados apresentaram graus de similaridade de 93,1% a 95,0% em nível de nucleotídeos, e de 82,3% a 89,0% em nível de aminoácidos, revelando estreito relacionamento (Silva-Rosales et al, 2000).…”

“…Quando comparadas conjuntamente com as de sete isolados asiáticos, as seqüências de genes cp dos isolados mexicanos de PRSV-P estudados apresentaram graus de similaridade de 93,1% a 95,0% em nível de nucleotídeos, e de 82,3% a 89,0% em nível de aminoácidos, revelando estreito relacionamento (Silva-Rosales et al, 2000).…”

Resistencia de Vasconcellea cauliflora al Virus de la mancha anular de la papaya-potyvirus (PRSV-P) y su introgresión en Carica papaya

“…Actualmente se reportan cinco grupos genéticos que se distribuyen en la costa del Pacifico, la costa del Golfo, regiones de Cotaxtla (Veracruz), la península de Yucatán y la costa occidental del Pacifico (Noa-Carranza et al, 2006). Dentro de esa diversidad, dos aislamientos de Veracruz y una de Chiapas están fuertemente correlacionadas con aislamientos de América del Norte y Australia; a la vez, todos estos aislamientos son genéticamente diferentes a los de China, Taiwán, Vietnam e India (Silva-Rosales et al, 2000).…”

“…En contraposición, los análisis de nucleótidos de los genes de proteína de la cápside de muestras virales colectadas en Cuba (7 muestras) y Brasil (21 muestras), indicaron que la distancia genética entre ellas varió de 0% a 9.2%. Los análisis dentro de cada país America and Australia, they are also genetically different from those from China, Taiwan, Vietnam and India (Silva-Rosales et al, 2000).…”