Etiologia e estratégias de controle de viroses do mamoeiro no brasil

Lima, Roberto César Pereira; Lima, J.A.; Souza, M.T.; Pio-Ribeiro, Gilvan; Andrade, Gilcifran Prestes de

doi:10.1590/s0100-41582001000400001

Cited by 32 publications

(48 citation statements)

References 23 publications

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“…When this latex is oxidized, the fruit surface becomes darkened and the pulp is damaged, reducing the fruit's commercial value . The removal and destruction of diseased plants is the only method available for controlling Papaya meleira disease (Lima et al, 2001;Ventura et al, 2004). Although the transmission mechanism has been examined in previous studies, it is not well understood (Rodrigues et al, 2009;Abreu et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Early diagnosis of a Mexican variant of Papaya meleira virus (PMeV-Mx) by RT-PCR

et al. 2015

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ABSTRACT. Papaya meleira disease was identified in Brazil in the 1980s. The disease is caused by a double-stranded RNA virus known as Papaya meleira virus (PMeV), which has also been recently reported in Mexico. However, previously reported PMeV primers failed to diagnose the Mexican form of the disease. A genomic approach was used to identify sequences of the Mexican virus isolate, referred here to as PMeV-Mx, to develop a diagnostic method. A mini cDNA library was generated using total RNA from the latex of fruits; this RNA was also sequenced using the Illumina platform. Sequences corresponding to the previously reported 669-base pair sequence for PMeV from Brazil (PMeV-Br) were identified within the PMeV-Mx genome, exhibiting 79-92% identity with PMeV-Br. In addition, a new sequence of 1154-base pairs encoding a putative RNA-dependent RNA polymerase was identified in PMeV-Mx. Primers designed against this sequence detected both virus isolates, 2 amplicons of 173 and 491 base pairs from PMeV-Br and PMeV-Mx, and shared 100 and 98% identity, respectively. PMeV-Mx was found in the latex of fruits, in seedlings, 1145-1154 (2015) and in the leaves, flowers, petioles, and seeds of mature plants. PMeVMx was more abundant in the latex of fruits than in the leaves. The limit of detection of the CB38/CB39 primer pair was 1 fg and 1 pg using total RNA extracted from the latex of fruits and from seedlings, respectively. A sensitive and early diagnosis protocol was developed; this method will enable the certification of seeds and seedlings prior to transplantation to the field.

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Section: Introductionmentioning

confidence: 99%

Early diagnosis of a Mexican variant of Papaya meleira virus (PMeV-Mx) by RT-PCR

et al. 2015

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“…Initial infection with the virus manifests as yellowing of the younger leaves, which later progresses to more severe symptoms of curled leaves, wilting and senescence. Green spots are commonly found on immature fruit and they turn yellow as the fruit reaches maturity (Lima et al, 2001). PLYV is mechanically transmitted and can be found in the soil (Camarço et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Genetic variability of papaya lethal yellowing virus isolates from Ceará and Rio Grande do Norte States, Brazil

Daltro¹,

Pereira²,

Cascardo³

et al. 2012

Trop. plant pathol.

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The papaya (Carica papaya) is a fruit crop of great economic importance throughout the Brazilian northeast, which is responsible for 60% of the national output. Papayas in the states of Ceará and Rio Grande do Norte are affected by lethal yellowing disease, caused by papaya lethal yellowing virus (PLYV). Previous work suggested that PLYV is a putative sobemovirus. To assess the genetic variability of PLYV, foliar samples were collected in October 2008 and October 2009 in commercial fields from Ceará and Rio Grande do Norte states, and total RNA was extracted. Specific primers based on the sequence of a previously characterized PLYV isolate were used for the RT-PCR-based amplification of a 900 bp fragment corresponding to the central region of the viral genome. Fragments from 21 viral isolates were cloned and sequenced. Sequence analyses indicated >97% nucleotide sequence identity among the isolates, 94-100% identity with the previously sequenced PLYV isolate, and a lower but significant identity with sobemoviruses (43-48.5%). These results suggest a low genetic variability among PLYV isolates, and are in agreement with the provisional placement of PLYV in the genus Sobemovirus. Definitive taxonomic conclusions, however, can only be drawn after the determination of the full-length genomic sequence. Key words: Carica papaya, PLYV, sobemovirus. RESUMO Variabilidade genética de isolados do papaya lethal yellowing virus dos estados do Ceará e Rio Grande do Norte, BrasilO mamão (Carica papaya) é uma fruta de grande importância econômica em todo o Nordeste brasileiro, região responsável por 60% da produção nacional. Mamoeiros nos estados do Ceará e Rio Grande do Norte são afetados pela doença denominada amarelo letal do mamoeiro, causada pelo papaya lethal yellowing virus (PLYV). Trabalhos anteriores indicaram que o PLYV é um possível sobemovírus. A fim de estimar a variabilidade genética do PLYV, amostras foliares de mamoeiro foram coletadas em outubro de 2008 e de 2009 em regiões produtoras do Ceará, e Rio Grande do Norte. Oligonucleotídeos específicos baseados na sequência de um isolado de PLYV previamente caracterizado foram utilizados para a amplificação via RT-PCR de um fragmento com 900 pb, correspondente à região central do genoma viral. Fragmentos de 21 isolados virais foram clonados e sequenciados. A análise das sequências de nucleotídeos indicou >97% de identidade entre os isolados, entre 94-100% com o isolado de PLYV previamente sequenciado, e uma identidade baixa, porém significativa, com sobemovírus (43-48,5%). Estes resultados sugerem um baixo grau de variabilidade genética entre isolados de PLYV, e estão de acordo com a classificação provisória do PLYV como membro do gênero Sobemovirus. Conclusões de ordem taxonômica, no entanto, só podem ser tiradas após a determinação da sequência genômica completa.

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“…Com a expansão da cultura do mamoeiro (Carica papaya L.) em áreas irrigadas do Nordeste brasileiro, as infecções causadas por bactérias, fungos, nematóides e vírus podem trazer sérios problemas de ordem sanitária para a cultura (Camarço, 1997;Lima et al, 2001;Lima & Lima, 2002a, 2002b. As viroses têm sido consideradas como as principais doenças do mamoeiro, ocasionando grandes perdas na sua produção (Lima et al, 2001;Lima & Lima, 2002a, 2002b e impossibilitando seu cultivo em áreas extensas, imprimindo um caráter itinerante ou nômade à cultura (Camarço et al, 1998;Lima et al, 2001;Lima & Lima, 2002a, 2002b.…”

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“…As viroses têm sido consideradas como as principais doenças do mamoeiro, ocasionando grandes perdas na sua produção (Lima et al, 2001;Lima & Lima, 2002a, 2002b e impossibilitando seu cultivo em áreas extensas, imprimindo um caráter itinerante ou nômade à cultura (Camarço et al, 1998;Lima et al, 2001;Lima & Lima, 2002a, 2002b.…”

unclassified

“…Dentre os principais vírus que infetam o mamoeiro, somente o vírus da mancha anelar do mamoeiro, tipo Papaya (Papaya ringspot virus, PRSV-P), família Potyviridae, gênero Potyvirus; o vírus do amarelo letal do mamoeiro ("Papaya lethal yellowing virus", PLYV), possível família Tombusviridae, gênero Carmovirus e o vírus da meleira, sem definição taxonômica foram registrados em pomares brasileiros (Camarço et al, 1998;Lima et al, 2001). O PLYV é responsável por importante virose no mamoeiro (Kitajima et al, 1992a;Lima et al, 1994;Camarço et al, 1998;Lima et al, 2001;Lima & Lima, 2002a, 2002b.…”

unclassified

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Transmissão por mãos contaminadas e ausência de transmissão embrionária do vírus do amarelo letal do mamoeiro

Saraiva

Paiva

Filho³

et al. 2006

Fitopatol. bras.

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RESUMOO vírus do amarelo letal do mamoeiro ("Papaya lethal yellowing virus", PLYV) é responsável por uma das principais doenças do mamoeiro (Carica papaya) no Nordeste brasileiro. O PLYV pode ser transmitido por solo, água, instrumentos de corte contaminados e por inoculação mecânica em condições experimentais. Na presente pesquisa, avaliou-se a transmissão do PLYV por mãos contaminadas e sua presença em embriões de sementes de frutos infetados. O PLYV foi transmitido por mãos contaminadas, e mãos contaminadas e lavadas em água corrente revelando elevada estabilidade. Experimentos com 1.128 embriões de sementes obtidas de frutos infetados com o vírus revelaram a ausência do PLYV, cuja presença foi constatada em 112 tegumentos das 670 sementes analisadas. O PLYV não foi perpetuado por plantio direto de sementes, envolvendo 310 plântulas e 362 mudas, comprovando não ser o mesmo transmitido por semente de forma embrionária. De outra parte, a presença do PLYV foi comprovada por Elisa indireto em 49 de 456 sementes per si testadas.Palavras-chave adidionais: Carica papaya, PLYV, Tombusviridae, Papaya lethal yelloing virus. ABSTRACT Transmission by contaminated hands and absence of embryonary seed transmission of Papaya lethal yellowing virusThe "Papaya lethal yellowing virus" (PLYV) is responsible for an important disease of papaya (Carica papaya) in Northeast Brazil. PLYV can be transmitted by soil, water and contaminated cutting instruments, as well as through mechanical inoculation. The objective of the present work was to evaluate the possibility of the virus transmission by contaminated hands, and its presence in the embryos of infected papaya fruit seeds. The virus was transmitted through contaminated hands, thus revealing that PLYV is very stable. Experiments with embryos from 1,128 seeds of infected fruits revealed the absence of PLYV in the embryos from seeds of infected fruits, although the virus was present in 112 out of the 670 seed peel samples analyzed. Direct planting of seeds from infected fruits, involving 310 seedlings and 362 plant-seedlings, did not perpetuate PLYV, demonstrating that the virus is not transmitted in the embryo of the seeds. On the other hand, PLYV was detected by indirect Elisa in 49 out of 456 seeds per si.

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Etiologia e estratégias de controle de viroses do mamoeiro no brasil

Cited by 32 publications

References 23 publications

Early diagnosis of a Mexican variant of Papaya meleira virus (PMeV-Mx) by RT-PCR

Early diagnosis of a Mexican variant of Papaya meleira virus (PMeV-Mx) by RT-PCR

Genetic variability of papaya lethal yellowing virus isolates from Ceará and Rio Grande do Norte States, Brazil

Transmissão por mãos contaminadas e ausência de transmissão embrionária do vírus do amarelo letal do mamoeiro

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