Coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (OYDV)

Kobayashi, Ken; Rabinowicz, Pablo D.; Bravo-Almonacid, Fernando; Helguera, Marcelo; Conci, V. C.; Lot, Hervé; Mentaberry, Alejandro

doi:10.1007/bf01718630

Cited by 23 publications

(14 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…no. X89402 (Kobayashi et al, 1996) and are situated in the 3 UTR and NIb region respectfully. c Diagnostic primers Xho5-13 and Nde 5-13 are based on clone 5-13 sequence.…”

Section: Rt-pcr Primersmentioning

confidence: 99%

See 1 more Smart Citation

Molecular characterisation of Onion yellow dwarf virus (OYDV) infecting garlic (Allium sativum L.) in Israel: Thermotherapy inhibits virus elimination by meristem tip culture

Shiboleth

Gal‐On

Koch

et al. 2001

Annals of Applied Biology

View full text Add to dashboard Cite

Garlic (cv. Shani) was tested using single step RT-PCR and digoxygenin (DIG) labelled dot-blot for a number of viruses. Following sequence analysis it was shown that at least three different polymorphs of the potyvirus Onion yellow dwarf virus (OYDV) infect the same plant simultaneously, together with the potyvirus Leek yellow stripe virus (LYSV), the carlavirus Garlic common latent virus (GCLV) and a multitude of allexiviruses [Shallot virus X (ShVX) related viruses]. Several garlic plants free of all the viruses tested were obtained through meristem-tip culture. Plants infected with single viruses or with different combinations of viruses were similarly obtained. Meristem-tip culture was confirmed as a satisfactory method of virus eradication, while thermotherapy treatment given to mother plantlets before meristem excision was found to specifically antagonise OYDV eradication. This work uses molecular methods for the first time to examine the effectiveness of meristem-tip culture for the eradication of multiple viruses from garlic.

show abstract

“…no. X89402 (Kobayashi et al, 1996) and are situated in the 3 UTR and NIb region respectfully. c Diagnostic primers Xho5-13 and Nde 5-13 are based on clone 5-13 sequence.…”

Section: Rt-pcr Primersmentioning

confidence: 99%

“…Based on comparisons of information from many published GenBank sequences, plus sense RNA viruses that infect garlic can be grouped into two families. Of the Potyviridae only two viruses have been identified in garlic, both potyviruses, Onion yellow dwarf virus (OYDV) (Kobayashi et al, 1996;GenBank acc. no.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterisation of Onion yellow dwarf virus (OYDV) infecting garlic (Allium sativum L.) in Israel: Thermotherapy inhibits virus elimination by meristem tip culture

Shiboleth

Gal‐On

Koch

et al. 2001

Annals of Applied Biology

View full text Add to dashboard Cite

show abstract

“…It is important for transmission of Potyviridae and, in addition, the degree of the identity between coat protein sequences can be used for the determination of relationship within the Potyviridae [135,136,137,138,139,140,141,142,143]. The role of CP and its binding with the helper component (HC) in aphid transmission is demonstrated [144,145,146].…”

Section: Introductionmentioning

confidence: 99%

Sharka: The Past, The Present and The Future

Sochor

Babula

Adam

et al. 2012

Viruses

View full text Add to dashboard Cite

Members the Potyviridae family belong to a group of plant viruses that are causing devastating plant diseases with a significant impact on agronomy and economics. Plum pox virus (PPV), as a causative agent of sharka disease, is widely discussed. The understanding of the molecular biology of potyviruses including PPV and the function of individual proteins as products of genome expression are quite necessary for the proposal the new antiviral strategies. This review brings to view the members of Potyviridae family with respect to plum pox virus. The genome of potyviruses is discussed with respect to protein products of its expression and their function. Plum pox virus distribution, genome organization, transmission and biochemical changes in infected plants are introduced. In addition, techniques used in PPV detection are accentuated and discussed, especially with respect to new modern techniques of nucleic acids isolation, based on the nanotechnological approach. Finally, perspectives on the future of possibilities for nanotechnology application in PPV determination/identification are outlined.

show abstract

“…The identification and characterization of viruses infecting garlic are based on serology, host range and sequence data of coat protein (Kobayashi et al, 1996;Tsuneyoshi et al, 1998;Fajardo et al, 2001). Recent advancements in molecular biology have provided a new paradigm for the classification and identification of viruses.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Phylogenetic Analysis of Coat Protein Gene of <I>Leek Yellow Stripe Virus</I> Infecting Garlic in India

Gupta¹,

Jain²,

Rao³

2017

View full text Add to dashboard Cite

The coat protein (CP) gene of two isolates of Leek yellow stripe virus (LYSV) from India was sequenced and found to be 864 bp, encoding a protein with 288 amino acids. The CP sequences of both the isolates were deposited in GenBank with accession numbers KF724857 and KP168262 corresponding to the isolates AC-50 and PGS-14, respectively. The Indian isolates of LYSV shared maximum nucleotide (nt) and amino acid (aa) identities of 85% and 90% respectively with that of Myanmar isolate (AB551622). Nt and aa based sequence identities of two Indian LYSV isolates with the corresponding sequences of 34 other LYSV isolates from worldwide revealed that, Indian isolate shared 77-84% and 80-90% identity respectively among themselves. It showed 5.3 % diversity between the Indian isolates and 23 % diversity among the isolates reported worldwide. Amino acid sequence comparison showed high variability at N-terminal region of CP gene of LYSV. Phylogenetic analysis of CP sequences of 36 isolates comprising India and other isolates from world segregated them into two major groups (major group I and major group II). The Indian isolates were clustered with isolates of Myanmar (AB551622) and Japan (AB194640) in subgroup III of major group II. The phylogenetic analysis revealed that Indian isolate is closely related to an isolate from Myanmar (AB551622). The present study comprises the first report on unravelling the molecular variability existing among the LYSV isolates.

show abstract

Coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (OYDV)

Cited by 23 publications

References 27 publications

Molecular characterisation of Onion yellow dwarf virus (OYDV) infecting garlic (Allium sativum L.) in Israel: Thermotherapy inhibits virus elimination by meristem tip culture

Molecular characterisation of Onion yellow dwarf virus (OYDV) infecting garlic (Allium sativum L.) in Israel: Thermotherapy inhibits virus elimination by meristem tip culture

Sharka: The Past, The Present and The Future

Molecular Characterization and Phylogenetic Analysis of Coat Protein Gene of <I>Leek Yellow Stripe Virus</I> Infecting Garlic in India

Contact Info

Product

Resources

About