Coagulase Typing and spa Typing of Methicillin Resistant Staphylococcus aureus: Relatedness Among Patients' and Carrier Strains

Abstract

This study was carried out to determine the types of Methicillin-Resistant Staphylococcus aureus (MRSA) by two typing systems, Coagulase typing and spa typing and to identify relationship between types of MRSA strains isolated from patients and hospital staff carriers. A total of 40 MRSA strains, 33 from cases of wound infection and 7 from anterior nares of hospital staffs were investigated. Coagulase typing showed that all the MRSA strains isolated from patients were coagulase type VI and all the strains isolated from carriers were coagulase type VII. In spa typing, seven spa types were detected such as S4, S6, S7, S8, S9, S10 and S11. Predominant spa type in patients was S7 detected in 15 (45.45%) MRSA isolates, followed by S4 in 8 (24.24%) strains and S6 in 5 (15.15%) strains. Other spa types detected in patients in small numbers were S8 (3.03%), S9 (3.03%), S10 (6.06%) and S11 (3.03%). While in strains of MRSA isolated from carriers, only two types S4 and S9 were detected. Both the spa types identified from carriers were also detected in a total of 9 (27.2%) of MRSA strains isolated from patients.

Pattern of Aerobic Bacterial Pathogens Causing Wound Infection at a Tertiary Teaching Hospital in Bangladesh with Special Reference to Methicillin Resistant Staphylococcus aureus and Extended Spectrum Beta Lactamases

“…Among them 15(65.22%) are isolated from surgical site infection, 2(66.67%) from traumatic wound, 3((100%) from burn, but none from abscess. Our study is nearly similar with the Khan et al 24 and Afroz 20 in Bangladesh and their finding was 62.5% and 70.2% respectively. But different finding was reported by Jahan et al 25 in Bangladesh (88.3%) and Vidhani et al 22 in India (51.6%).…”

“…Methicillin resistant Staphylococcus aureus (MRSA) and extended spectrum beta lactamase (ESBL) producing gram negative bacteria are the main concern among the drug resistant isolates. Methicillin-resistant Staphylococcus aureus is a major nosocomial pathogen worldwide and is potentially a great threat in medical therapy 20 . MRSA infections are increasingly reported from many countries world wide 21 .…”

“…MRSA infections are increasingly reported from many countries world wide 21 . Different studies showed that MRSA was 51.6% cases in India 22 , 50.0% cases in Pakistan 23 and 62.5% cases, 70.2% cases and 83.3% cases 20,[24][25] in Bangladesh. The molecular mechanism responsible for methicillin resistance is the presence of the staphylococcal cassette chromosome (SCC), a mobile element in the genome of Staphylococcus aureus.…”