Coagulase-Positive Staphylococci Isolated from Chicken Meat: Pathogenic Potential and Vancomycin Resistance

Martins, Paula Dalcin; Almeida, Taiana Trindade de; Basso, Ana Paula; Moura, Tiane Martin de; Frazzon, Jeverson; Tondo, Eduardo César; Frazzon, Ana Paula Guedes

doi:10.1089/fpd.2013.1492

Cited by 34 publications

(31 citation statements)

References 40 publications

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“…Staphylococcus are a genre of bacteria widespread in nature, being found in the air, soil, water, food products and animals, including human beings (Faria et al 2009, Hou et al 2012, Martins et al 2013. In this study the frequency to CNS species was higher than CPS, this result agrees with Serapicos (2008) and Faria et al (2009) who also demonstrated an elevated frequency of CNS in wastewater.…”

Section: Resultssupporting

confidence: 87%

Antibiotic resistance and enterotoxin genes in Staphylococcus sp. isolates from polluted water in Southern Brazil

Basso¹,

Martins²,

Nachtigall³

et al. 2014

An. Acad. Bras. Ciênc.

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The aim of this study was to evaluate the species distribution, antibiotic-resistance profile and presence of enterotoxin (SE) genes in staphylococci isolated from the Dilúvio stream in South Brazil. Eighty-eight staphylococci were identified, 93.18% were identified as coagulase-negative (CNS) and 6.82% coagulasepositive (CPS). Fourteen Staphylococcus species were detected and the most frequently were Staphylococcus cohnii (30.48%) and S. haemolyticus (21.95%). Resistance to erythromycin was verified in 37.50% of the strains, followed by 27.27% to penicillin, 12.50% to clindamycin, 6.81% to trimethoprim-sulfamethoxazole, 5.68% to chloramphenicol and 2.27% to norfloxacin. None of the investigated strains showed gentamicin and ciprofloxacin resistance. The strains were tested for the presence of sea, seb, sec, sed and see genes by PCR and only CNS strains (43.18%) showed positive results to one or more SE genes. The scientific importance of our results is due to the lack of data about these topics in polluted waters in Brazil. In conclusion, polluted waters from the Dilúvio stream may constitute a reservoir for disseminating antibiotic-resistance and enterotoxin into the community. In addition, the detection of staphylococci in the polluted waters of the Dilúvio stream indicated a situation of environmental contamination and poor sanitation conditions.

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Section: Resultssupporting

confidence: 87%

Antibiotic resistance and enterotoxin genes in Staphylococcus sp. isolates from polluted water in Southern Brazil

Basso¹,

Martins²,

Nachtigall³

et al. 2014

An. Acad. Bras. Ciênc.

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“…Antimicrobial resistance was mainly against penicillin (94%), clindamycin (90%), and sulfamethoxazole/trimethoprim (82%). Vancomycin-resistant S. aureus showed intermediate resistance (51%), which might indicate the dissemination of vancomycin resistance in the community and imply food-safety hazards (Martins et al, 2013). The percentage of resistance to b-lactams was variable, with the highest resistance being to penicillin (94%) and lowest to ampicillin-sulbactam (22%).…”

Section: Resultsmentioning

confidence: 99%

Prevalence and Antimicrobial Resistance Profile of Staphylococcus Species in Chicken and Beef Raw Meat in Egypt

Osman

Amer

Badr³

et al. 2015

Foodborne Pathogens and Disease

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Coagulase-positive (CPS) and coagulase-negative (CNS) staphylococci cause staphylococcal food poisoning. Recently, CPS and CNS have received increasing attention due to their potential role in the dissemination of antibiotic resistance markers. The present study aimed to evaluate CPS and CNS species distribution and their antibiotic resistance profile isolated from chicken and beef meat. Fifty fresh, uncooked chicken parts and 50 beef meat cuts (local n=27; imported n=23) were used. One hundred staphylococcal isolates belonging to 11 species were isolated and identified from chicken (n=50) and beef (n=50) raw meat samples. Staphylococcus hyicus (26/100), lugdunensis (18/100), aureus (15/100) and epidermidis (14/100) were dominant. S. aureus was 100% resistant to penicillin and sulfamethoxazole/trimethoprim. Vancomycin-resistant S. aureus showed intermediate resistance (51%), which might indicate the dissemination of vancomycin resistance in the community and imply food safety hazards. The percentage of resistance to β-lactams was variable, with the highest resistance being to penicillin (94%) and lowest to ampicillin-sulbactam (22%). Antimicrobial resistance was mainly against penicillin (94%), clindamycin (90%) and sulfamethoxazole/trimethoprim (82%). The results indicate that chicken and beef raw meat are an important source of antibiotic-resistant CPS and CNS.

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“…Coagulase-negative staphylococci (CoNS) , including many species such as S. hyicus [3], S. gallinarum [4], S.xylosus and S. epidermidis [5,6], and commonly been isolated from the nares and skin of healthy chickens, and their taxonomic positions were discussed apart from the pathogenicities. CoNS had been isolated from frozen and chilled industrialized, uncooked chicken parts or entire carcasses [7] , raw chicken's meat [8,9] , meat product [10] , cooked chicken products [11] , breast, neck and wing of chickens [12] , chicken carcasses herd-wise pooled neck skin samples [13] as well as poultry bioaerosol [14] .…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of antibiotic resistance genes in identified of Coagulase Negative Staphylococci from chickens flocks and hatcheries in Egypt.

Shokry

Redwan²,

Amer

et al. 2018

NIDOC-ASRT

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T HE COLLECTED 942 samples (6 organ/bird) from 157 birds taken from 34 broiler chicken farms aged 2-31 days including 13 and 21 clinical diseased and apparently health flock as well as 221 hatchery samples. Samples were tested for isolation of Coagulase negative staphylococcus (CoNS).Results of isolation from chicken flocks with clinical signs are 9/13(69.23%) were positive. Positive samples (11/354, 3.11%) including : 3liver, 4 kidney, 2 intestine, 1 air sac and 1 nasal sinus. Out of apparent healthy flocks 8/21 (38.95%) were positive. Positive samples (15/ 588, 2.55%) including 3 liver, 2 kidney, 4 intestine, 3 lung, 2 air sac and 1 nasal sinus. Results showed that 15 positive flocks out of 34 flocks (34.09%) with 26 Staphylococcus isolates from 942 samples (2.77%) including 6 liver, 6 kidney, 6 intestine, 4 lung, 2 air-sacs and 2 nasal sinus with rate of 3.82%, 3.82%, 3.82%, 2.55%, 1.27% and 1.27%; respectively. Also12 isolates out of 26 (46.15%) were CoNS include 8 S. xylosus (75 %), 2 S. scuiri (16.67%) and 2 S. lentus (16.67%).Hatchery samples reveals the isolation of 26 staphylococcus isolates (11.76%). The tested 108 fertile eggs and dead in shell embryos resulted in 14 and 12 isolates in rate of 12.96% and 13.79%; respectively. Ten isolates were CoNS (38.46%) and represented 4.52% out of total samples including 8 S. xylosus and 2 S. scuiri. Eight S. xylosus was 6 (5.55%) from infertile eggs and 2 (2.29 %) from dead in shell, While the 2 S. scuiri (1.85%) were obtained from infertile eggs.The tested CoNS isolates showed 100% resistance to Oxytetracycline 30 µg/ml (T30), Trimethoprime + Sulphamethexole 2.25/23.75 µg/ml (SXT) , Calindamycin 2 µg/ml (DA) and Oxacillin 30 µg/ml (OX). All isolates were 100% susceptible to Vancomycin 30 µg/ ml (VA) and 90% susceptibility to Enrofloxacin 5 µg/ml (ENR). Multidrug resistance was detected in form of resistance to 9, 4 and 5 out of tested 13 antibiotics in 2 S.lentus , 5 S. scuiri and 15 S. xylosis; respectively.Ten isolates were tested for the presence of 7 resistance gens including: mecA , tetK, blaZ , kan , ermC, icaD, bab gene. Seven isolates from the tested 10 (70%) having 4 resistance gens. The most detected gens are mecA tetK, blaZ and ermC where it was detected in 90, 80, 60 and 90% respectively. Kan , icaD and bab gens were detected in rate of 30.0 and 0 %; respectively.In conclusion: CoNS could isolated from healthy and diseased chicken flocks as well as from chicken hatchery. The obtained isolates were multidrug either phenotypic and /or genotypic resistant. Good hygienic measures in both chicken farms and hatchery with monitoring of drug resistance of CoNS those act as source for resistance genes to bacterial pathogens and their importance to the poultry and public health are recommended.

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Coagulase-Positive Staphylococci Isolated from Chicken Meat: Pathogenic Potential and Vancomycin Resistance

Cited by 34 publications

References 40 publications

Antibiotic resistance and enterotoxin genes in Staphylococcus sp. isolates from polluted water in Southern Brazil

Antibiotic resistance and enterotoxin genes in Staphylococcus sp. isolates from polluted water in Southern Brazil

Prevalence and Antimicrobial Resistance Profile of Staphylococcus Species in Chicken and Beef Raw Meat in Egypt

Molecular detection of antibiotic resistance genes in identified of Coagulase Negative Staphylococci from chickens flocks and hatcheries in Egypt.

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