Coactosin-like protein 1 inhibits neuronal migration during mouse corticogenesis

Li, Guohong; Yin, Yupeng; Chen, Jiong; Fan, Yanle; Ma, Juhong; Huang, Yingxue; Chen, Chen; Dai, Pengxiu; Chen, Shulin; Zhao, Shanting

doi:10.4142/jvs.2018.19.1.21

Cited by 13 publications

(9 citation statements)

References 28 publications

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“… 34 , 35 Therefore, none of these proteins appear functionally relevant with the cardiac fibrotic phenotype. Interestingly, COTL1 was reported to be involved in TGF-β signaling 36 and inhibit neuronal migration during mouse corticogenesis, 37 suggesting that COTL1 could be a Cfast binding protein to regulate cardiac fibrosis. To confirm the mass spectrometry data, we performed a Cfast RNA pull-down assay coupled with western blotting to detect COTL1 after the lncRNA Cfast pull-down.…”

Section: Resultsmentioning

confidence: 99%

Long noncoding RNA Cfast regulates cardiac fibrosis

Zhang

Kataoka

et al. 2021

Molecular Therapy - Nucleic Acids

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Cardiac fibrosis occurs in most cardiac diseases, which reduces cardiac muscle compliance, impairs both systolic and diastolic heart function and, ultimately, leads to heart failure. Long noncoding RNAs (lncRNAs) have recently emerged as important regulators of a variety of biological processes; however, little is known about the expression and function of lncRNAs in cardiac fibrosis. Using unbiased transcriptome profiling in a mouse model of myocardial infarction (MI), we identified a cardiac fibroblast-enriched lncRNA (AK048087) named cardiac fibroblast-associated transcript ( Cfast ), which is significantly elevated after MI. Silencing Cfast expression by small interfering RNAs (siRNAs) or lentiviral short hairpin RNAs (shRNAs) resulted in suppression of fibrosis-related gene expression and transdifferentiation of myofibroblasts into cardiac fibroblasts. Depletion of Cfast by lentiviral shRNAs in mouse hearts significantly attenuated cardiac fibrosis induced by MI or isoproterenol-infusion. Importantly, inhibition of Cfast ameliorated cardiac function following cardiac injury. RNA pull-down followed by mass spectrometry analyses identified COTL1 (coactosin-like 1) as one of the Cfast interacting proteins. Mechanistically, Cfast competitively inhibits the COTL1 interaction with TRAP1 (transforming growth factor-β receptor-associated protein 1), which enhances TGF-β signaling by augmenting SMAD2/SMAD4 complex formation. Therefore, our study identifies Cfast as a novel cardiac fibroblast-enriched lncRNA that regulates cardiac fibroblast activation in response to pathophysiological stress. Cfast could serve as a potential therapeutic target for the prevention of cardiac fibrosis and cardiac diseases.

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Section: Resultsmentioning

confidence: 99%

Long noncoding RNA Cfast regulates cardiac fibrosis

Zhang

Kataoka

et al. 2021

Molecular Therapy - Nucleic Acids

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“…Early- and late-born cortical neurons display distinct migratory behaviors 63 . A study conducted by Li et al revealed that COTL1 overexpression impaired migration of both early- and late-born neurons during mouse corticogenesis, which suggests COTL1 was required for both somal translocation and locomotion, which can further impact cognitive/emotional/behavioral development 64 . Additionally, a study conducted by Bosch et al shows that miR-30b was downregulated in the VTA of methamphetamine self-administering rats 65 .…”

Section: Discussionmentioning

confidence: 99%

Investigating the influence of perinatal nicotine and alcohol exposure on the genetic profiles of dopaminergic neurons in the VTA using miRNA–mRNA analysis

Kazemi

Huang

Avci

et al. 2020

Sci Rep

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Nicotine and alcohol are two of the most commonly used and abused recreational drugs, are often used simultaneously, and have been linked to significant health hazards. Furthermore, patients diagnosed with dependence on one drug are highly likely to be dependent on the other. Several studies have shown the effects of each drug independently on gene expression within many brain regions, including the ventral tegmental area (VTA). Dopaminergic (DA) neurons of the dopamine reward pathway originate from the VTA, which is believed to be central to the mechanism of addiction and drug reinforcement. Using a well-established rat model for both nicotine and alcohol perinatal exposure, we investigated miRNA and mRNA expression of dopaminergic (DA) neurons of the VTA in rat pups following perinatal alcohol and joint nicotine–alcohol exposure. Microarray analysis was then used to profile the differential expression of both miRNAs and mRNAs from DA neurons of each treatment group to further explore the altered genes and related biological pathways modulated. Predicted and validated miRNA-gene target pairs were analyzed to further understand the roles of miRNAs within these networks following each treatment, along with their post transcription regulation points affecting gene expression throughout development. This study suggested that glutamatergic synapse and axon guidance pathways were specifically enriched and many miRNAs and genes were significantly altered following alcohol or nicotine–alcohol perinatal exposure when compared to saline control. These results provide more detailed insight into the cell proliferation, neuronal migration, neuronal axon guidance during the infancy in rats in response to perinatal alcohol/ or nicotine–alcohol exposure.

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“…Thus, this new PCV species was designated PCV3 [22]. The virus has since been identified in multiple countries, including Germany [24], Japan [25], Korea [26], Russia [27], China [28], Thailand [29], Italy [30], Spain [31], Denmark [32], South Korea [33], Poland [34], Brazil [35], and Sweden [36]. While testing is somewhat limited to date, the finding of PCV3 in retrospective samples indicates that this species of circovirus has likely been circulating in swine populations decades prior to the initial detection and case reports [36].…”

Section: Introductionmentioning

confidence: 99%

PCV3-associated disease in the United States swine herd

Arruda

Piñeyro

Derscheid

et al. 2019

Emerging Microbes & Infections

134

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Porcine circovirus-associated disease encompasses multiple disease syndromes including porcine circovirus 2 systemic diseases, reproductive failure, and porcine dermatitis and nephropathy syndrome. Until recently, porcine circovirus 2 was the only species associated with the porcine circovirus-associated disease. In this report, diagnostic investigations of thirty-six field cases submitted from multiple production systems, numerous sites and varied geographic locations demonstrated porcine circovirus 3 within lesions by in situ hybridization including fetuses with myocarditis, weak-born neonatal piglets with encephalitis and myocarditis, from cases of porcine dermatitis and nephropathy syndrome, and in weaned pigs with systemic periarteritis. Porcine circovirus 3 was detected by PCR in numerous fetuses and perinatal piglets at high viral loads (trillions of genome copies per mL of tissue homogenate). Samples from all cases in this study were assayed and found negative for porcine circovirus 2 by PCR. Metagenomic sequencing was performed on a subset of reproductive cases, consisting of sixteen fetuses/fetal sample pools. PCV3 was identified in all pools and the only virus identified in fourteen pools. Based on these data, porcine circovirus 3 is considered a putative cause of reproductive failure, encephalitis and myocarditis in perinatal piglets, porcine dermatitis and nephropathy syndrome, and periarteritis in swine in the United States.

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Coactosin-like protein 1 inhibits neuronal migration during mouse corticogenesis

Cited by 13 publications

References 28 publications

Long noncoding RNA Cfast regulates cardiac fibrosis

Long noncoding RNA Cfast regulates cardiac fibrosis

Investigating the influence of perinatal nicotine and alcohol exposure on the genetic profiles of dopaminergic neurons in the VTA using miRNA–mRNA analysis

PCV3-associated disease in the United States swine herd

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