Co-Treatment with Cefotaxime and High-Fructose Diet Inducing Nonalcoholic Fatty Liver Disease and Gut Microbial Dysbiosis in Mice

Abstract

High fructose diet causes metabolic syndrome and induces host gut microbial dysbiosis and related obesity and nonalcoholic fatty liver disease (NAFLD). Several antibiotic treatments could prevent fatty liver. However, there are studies that have demonstrated that a high-fructose diet could influence the gut microbial dysbiosis and induce fatty liver. The purpose of this study was performed to partially modify the gut bacterial composition with a single cefotaxime treatment, which might affect the fructose-induced NAFLD severity. The C57BL/6JNarl male mice were divided into four groups including vehicle/chow diet (VE-CD), vehicle/high-fructose diet (VE-FD), antibiotic (cefotaxime (CF))/CD, and CF/FD. The results showed that body weight gain, moderate hepatic steatosis severity, epididymal white adipose tissue hypertrophy, and insulin resistance occurrence with NAFLD-related symptoms were observed only in the CF-FD group. The raised protein expression of hepatic lipogenesis was observed in the CF-FD group, but lipolysis protein expression was no difference. The diversity and composition of microbiota were significantly reduced in the CF-FD group. The Erysipelatoclostridium, Enterobacteriaceae, Lachnospiraceae, and Escherichia Shigella were in increased abundance in the feces of CF-FD group compared with VE-FD group. The novel model reveals that particular antibiotics such as cefotaxime co-treatment with high-fructose diet may affect the gut microbiota accelerating the NAFLD and obesity.

Broussonetia papyrifera Polysaccharide Alleviated Acetaminophen-Induced Liver Injury by Regulating the Intestinal Flora

“…At the genus level, the flora of 13 genera increased significantly after APAP treatment, and the 7 genera recovered to varying degrees after BPP treatment, including norank_f_norank_o_ Clostridia_UCG-014, Erysipelatoclostridium, Gordonibacter, norank_f_Eubacterium_coprostanoli genes_group, Eubacterium_nodatum_group, Family_XIII_AD3011_group, and Eubacterium_ brachy_group. These bacteria are closely related to liver injury or metabolism [47][48][49][50][51]. Furthermore, BPP increased the richness of Corynebacterium, Prevotellaceae_UCG-001, Alloprevotella, and Jeotgalicoccus, which was similar to the report that walnut green peel polysaccharide and Spirulina platensis polysaccharides have a hepatoprotective effect [11,52].…”