Co-production of MCR-1 and NDM-1 by Escherichia coli sequence type 31 isolated from a newborn in Moscow, Russia

Sulian, O. S.; Ageevets, Vladimir; Lazareva, I.; Gostev, Vladimir; Popov, D. A.; Vostrikova, T. Yu.; Sukhinin, A.A.; Lobzin, Yuri; Сидоренко, С В

doi:10.1016/j.ijid.2020.09.1422

Cited by 6 publications

(7 citation statements)

References 9 publications

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“…In this study, the prevalence of phenotypic colistin resistance and of the mobile colistin resistance ( mcr- 1) genes among clinical E. coli and K. pneumoniae isolates collected from hospitalized patients in Russia in 2013–2018 as part of sentinel surveillance was systematically assessed, and in-depth analysis of the genome sequences of mcr -bearing isolates and plasmids was performed. While there have been several reports of mcr-1 positive isolates recovered from human and animal sources in Russia [ 12 , 13 , 14 , 15 ], to our knowledge, this study is the first to report the prevalence and genomic context of mcr-1 in clinical isolates collected from geographically distant sites across Russia.…”

Section: Discussionmentioning

confidence: 91%

“…Several research groups have previously reported on the sporadic occurrence of mcr-1 in Russia and described the corresponding mcr -bearing plasmids [ 12 , 13 , 14 , 15 ]. In this study, we aimed to systematically assess the prevalence of colistin resistance and of mcr genes in clinical Escherichia coli and Klebsiella pneumoniae isolates from Russian sentinel surveillance and to determine phenotypic and genomic characteristics of mcr -carrying isolates and plasmids.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Epidemiology of mcr-1-Positive Escherichia coli and Klebsiella pneumoniae Isolates: Results from Russian Sentinel Surveillance (2013–2018)

et al. 2022

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Background: The dissemination of mobile colistin resistance (mcr) genes is a serious healthcare threat because polymyxins represent “last-line” therapeutics for multi-drug-resistant Gram-negative pathogens. This study aimed to assess the prevalence of colistin resistance and mcr genes and characteristics of clinical Escherichia coli (Eco) and Klebsiella pneumoniae (Kpn) isolates and plasmids carrying these genes in Russia. Methods: A total of 4324 Eco and 4530 Kpn collected in the frame of sentinel surveillance in 2013–2018 were tested for susceptibility to colistin and other antibiotics using the broth microdilution method. mcr genes were screened by real-time PCR. Phylogeny, genomic features and plasmids of mcr-positive isolates were assessed using whole-genome sequencing and subsequent bioinformatic analysis. Results: Colistin resistance was detected in 2.24% Eco and 9.3% Kpn. Twenty-two (0.51%) Eco and two (0.04%) Kpn from distant sites carried mcr-1.1. Most mcr-positive isolates co-harbored ESBLs and other resistance determinants to various antibiotic classes. The mcr-positive Eco belonged to 16 MLST types, with ST359 being most common; Kpn belonged to ST307 and ST23. mcr-1.1 was carried mainly in IncI2 (n = 18) and IncX4 (n = 5) plasmids highly similar to those identified previously in human, animal and environmental isolates. Conclusion: This study demonstrated a dissemination of “typical” mcr-bearing plasmids among diverse Eco and Kpn genotypes and across a wide geographic area in Russia. Given the frequent association of mcr with other resistance determinants and potential clinical impact, the continual surveillance of this threat is warranted.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology of mcr-1-Positive Escherichia coli and Klebsiella pneumoniae Isolates: Results from Russian Sentinel Surveillance (2013–2018)

et al. 2022

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“…Даже изоляты Eco-49 и Есо-84, выделенные в одном стационаре и содержащие идентичные плазмиды IncX4, относились к разным сиквенс-типам [42]. Для штаммов, выделенных от пациентов, был характерен несколько более высокий уровень ассоциированной резистентности к антибиотикам разных групп, так пять из семи изолятов проявляли устойчивость к цефалоспоринам III поколения, несли гены бета-лактамаз расширенного спектра группы СТХ-М и/или бета-лактамаз класса С. Особый интерес представляет, выделенный в г. Грозный у новорождённого ребенка с пороком сердца, изолят Eco-5571, у которого были одновременно выявлены гены mcr-1 и bla NDM-1 [43]. При этом ген mcr-1 был локализован на плазмиде практически идентичной плазмиде, выдев 2018 г. на Алтае от дикой птицы Чёрный коршун (Milvus migrans) [44].…”

Section: Discussionunclassified

Associated resistance of Escherichia coli isolated from humans and animals to polymyxin and beta-lactam antibiotics

Sulian

Ageevets²,

Sukhinin

et al. 2022

A&Ch

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Escherichia coli isolates from various sources from 2018 to 2019 were included in the study. Mcr-1 genes were found in two of 105 animal strains (2%) and seven of 928 human strains (0.8%). All mcr-1-positive strains showed a low level of resistance to colistin (MIC ranged from 4 to 8 µg/ml). Both strains isolated from animals remained sensitive to betalactam antibiotics and did not contain beta-lactamase genes. Beta-lactamases were absent only in one of the strains isolated from humans. Four strains were resistant to cephalosporins with sensitivity to carbapenems and carried class A (blaCTX-M-15 or blaCTX-M-1) or class C (blaCMY-2) extended-spectrum beta-lactamases genes. One strain showed resistance to cephalosporins and meropenem and contained four beta-lactamase genes: blaNDM-1, blaCTX-M-15, blaTEM-1B, and blaCMY-6. Only one strain isolated from animals remained sensitive to ciprofloxacin, the rest showed high level of resistance, had amino acid substitutions in the DNA gyrase genes or mutations leading to overexpression of the mdfA gene. In terms of resistance to aminoglycosides, the strains varied widely and carried up to four aminoglycoside-modifying enzyme genes. One strain isolated from humans showed resistance to tigecycline, but no genes conferring resistance to this antibiotic were found. The data obtained substantiate the need for extended studies on the molecular epidemiology of associated resistance to polymyxins and beta-lactams.

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“…Подавляющее число плазмид обладают конъюгативной активностью. Доминирующими и наиболее распространёнными группами плазмид являются IncHI2, IncI2, и IncX4, при этом плазмиды демонстрируют высокий уровень консервативности, а также отсутствие выраженных доминирующих генетических линий бактерий, с которыми бы эти типы плазмид ассоциировались [32,33].…”

Section: горизонтальный перенос генов Mcr-1unclassified

“…Описан изолят E.coli от новорожденного, находящегося в реанимации, демонстрирующий ко-продукцию mcr-1 и карбапенемазы NDM-1. Плазмида, несущая ген mcr-1, оказалась гомологичной плазмиде, описанной у изолята от чёрного коршуна [57].…”

Section: описания в россииunclassified

Dissemination of MCR-Type Genes Encoding Polymyxin Resistance in Russia and Across the Globe

Ageevets¹,

Sulyan

Lazareva³

et al. 2021

A&Ch

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The MCR-type polymyxin resistance gene localized on a mobile genetic element was described was described in 2015 for the first time. Publications, following the first description, demonstrated the global distribution of MCR-type genes and the role of animal husbandry in this process. At the same time, practical importance of polymyxins is growing, considering spread of genes of acquired resistance to carbapenem antibiotics. This review briefly summarizes the principal data on this issue.

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Co-production of MCR-1 and NDM-1 by Escherichia coli sequence type 31 isolated from a newborn in Moscow, Russia

Cited by 6 publications

References 9 publications

Molecular Epidemiology of mcr-1-Positive Escherichia coli and Klebsiella pneumoniae Isolates: Results from Russian Sentinel Surveillance (2013–2018)

Molecular Epidemiology of mcr-1-Positive Escherichia coli and Klebsiella pneumoniae Isolates: Results from Russian Sentinel Surveillance (2013–2018)

Associated resistance of Escherichia coli isolated from humans and animals to polymyxin and beta-lactam antibiotics

Dissemination of MCR-Type Genes Encoding Polymyxin Resistance in Russia and Across the Globe

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