The MCR-type polymyxin resistance gene localized on a mobile genetic element was described was described in 2015 for the first time. Publications, following the first description, demonstrated the global distribution of MCR-type genes and the role of animal husbandry in this process. At the same time, practical importance of polymyxins is growing, considering spread of genes of acquired resistance to carbapenem antibiotics. This review briefly summarizes the principal data on this issue.
The study aims to compare two colistin susceptibility measurement approaches: by disk diffusion test and using VITEK® 2 Compact instrument. We collected 103 E.coli isolates from 7 livestock farms in St. Petersburg and the Leningrad region. Minimum inhibitory concentration (MIC) of colistin was determined using disk diffusion test and VITEK® 2 Compact instrument and then compared with the reference broth microdilution method results. The processing of the results was based on the EUCAST recommendations. Eight E. coli isolates proved to be resistant to colistin when determining MIC in broth microdilutions. Two isolates (eco‐103 and eco‐151) carried the MCR‐1 gene. The identical isolates were tested by other methods to determine the MIC. The data obtained by the three methods were identical. This suggests that the disc diffusion test can be used to determine the MIC of colistin on E.coli. This method has several advantages over the reference method and VITEK‐2 Compact use since it is simultaneously cheap, fast, and accurate for determining the MIC of colistin.
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