Co-occurrence ofmcr-1and ESBL on a single plasmid inSalmonella enterica

Yang, Yongqiang; Zhang, Anyun; Ma, Sanyuan; Kong, Linghan; Li, Yunxia; Liu, Jinxin; Davis, Margaret A.; Guo, Xi; Liu, Bihui; Lei, Changwei; Wang, Hongning

doi:10.1093/jac/dkw243

Cited by 69 publications

(57 citation statements)

References 8 publications

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“…Interestingly, four base pair (AACA, 1612–1615) is consistently missed in the IS Ecp1 - bla CTX-M-55 - orf477 operon (3090 bp) in both pE15017 and pA31-12, in relative to the counterpart (3094 bp) in pHN122-1(Fig 3D) [31]. In fact, the IS Ecp1 - bla CTX-M-55 transposition unit flanked by DR was also recently observed in the Salmonella plasmid pSCS23 (KU934209) as shown in Fig 3 [29]. …”

Section: Resultsmentioning

confidence: 58%

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Dissemination and Mechanism for the MCR-1 Colistin Resistance

et al. 2016

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Polymyxins are the last line of defense against lethal infections caused by multidrug resistant Gram-negative pathogens. Very recently, the use of polymyxins has been greatly challenged by the emergence of the plasmid-borne mobile colistin resistance gene (mcr-1). However, the mechanistic aspects of the MCR-1 colistin resistance are still poorly understood. Here we report the comparative genomics of two new mcr-1-harbouring plasmids isolated from the human gut microbiota, highlighting the diversity in plasmid transfer of the mcr-1 gene. Further genetic dissection delineated that both the trans-membrane region and a substrate-binding motif are required for the MCR-1-mediated colistin resistance. The soluble form of the membrane protein MCR-1 was successfully prepared and verified. Phylogenetic analyses revealed that MCR-1 is highly homologous to its counterpart PEA lipid A transferase in Paenibacili, a known producer of polymyxins. The fact that the plasmid-borne MCR-1 is placed in a subclade neighboring the chromosome-encoded colistin-resistant Neisseria LptA (EptA) potentially implies parallel evolutionary paths for the two genes. In conclusion, our finding provids a first glimpse of mechanism for the MCR-1-mediated colistin resistance.

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Section: Resultsmentioning

confidence: 58%

“…Previously, the co-occurrence of ESBL and MCR-1 had been found on the IncHI2-type plasmids from E . coli and Salmonella enterica [20,29]. Similar to the other mcr-1 -carrying plasmid, pA31-12 (Fig 3D) [30], pE15017 might be an additive example of IncI2-type plasmid with above two antibiotic resistance determinants.…”

Section: Resultsmentioning

confidence: 99%

Dissemination and Mechanism for the MCR-1 Colistin Resistance

et al. 2016

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“…The IncI2 plasmid has also been found to carry blaKPC-3 or blaCTX-M genes including blaCTX-M-55 in isolates recovered pets and food animals in China (13). Furthermore, co-localization of mcr-1 with blaCTX-M-55 on IncI2 plasmid has been recognized in E. coli and Salmonella enterica isolates recovered from chicken in China (14,15). Other mcr-1-coding IncI2 plasmids derived from food animals in Japan have been recently reported by Suzuki et al (3).…”

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confidence: 99%

First Detection of an Escherichia coli Strain Harboring the mcr-1 Gene in Retail Domestic Chicken Meat in Japan

et al. 2017

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“…To the best of our knowledge, this is the first report of an IncY plasmid carrying the mcr-1 gene. To date, the mcr-1 gene has been found in seven different plasmid incompatibility groups (IncI2 [20], IncFII [21], IncX4 [22], IncHI1 [15], IncHI2 [23], IncP [22], and IncF [10]), suggesting the wide distribution and high dissemination capacity of mcr-1-carrying plasmids. Given the fact that many phage-related genes are encoded by pMCR-1-P3 and most are homologous to the complete E. coli genome (GenBank accession number CP007392), this plasmid was likely formed via homologous recombination from a prophage that was originally located in the E. coli genome.…”

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confidence: 99%

A Phage-Like IncY Plasmid Carrying the mcr-1 Gene in Escherichia coli from a Pig Farm in China

Zhang

Feng

Liu

et al. 2017

Antimicrob Agents Chemother

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We report here a new type of plasmid that carries the mcr-1 gene, the pMCR-1-P3 plasmid, harbored in an Escherichia coli strain isolated from a pig farm in China. pMCR-1-P3 belongs to the IncY incompatibility group and is a phage-like plasmid that contains a large portion of phage-related sequences. The backbone of this plasmid is different from that of other mcr-1-carrying plasmids reported previously.

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Co-occurrence ofmcr-1and ESBL on a single plasmid inSalmonella enterica

Cited by 69 publications

References 8 publications

Dissemination and Mechanism for the MCR-1 Colistin Resistance

Dissemination and Mechanism for the MCR-1 Colistin Resistance

First Detection of an <i>Escherichia coli</i> Strain Harboring the <i>mcr-1</i> Gene in Retail Domestic Chicken Meat in Japan

A Phage-Like IncY Plasmid Carrying the mcr-1 Gene in Escherichia coli from a Pig Farm in China

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