First Detection of an &lt;i&gt;Escherichia coli&lt;/i&gt; Strain Harboring the &lt;i&gt;mcr-1&lt;/i&gt; Gene in Retail Domestic Chicken Meat in Japan

Ohsaki, Yusuke; Hayashi, Wataru; Saito, Satomi; Osaka, Shunsuke; Taniguchi, Yui; Koide, Shota; Kawamura, Kazuo; Nagano, Yukiko; Arakawa, Yoshichika; Nagano, Noriyuki

doi:10.7883/yoken.jjid.2016.572

Cited by 26 publications

(18 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All seven colistin-resistant E. coli strains in this study were able to grow normally on MacConkey agar plates without colistin. Similarly, a CTX-M-1-producing E. coli harboring the mcr-1 gene on an IncI2 plasmid recovered from retail chicken meat reported previously could not grow on MacConkey agar with colistin but grew well on MacConkey agar with cefotaxime (18). Thus, it seems likely that those bacteria with outer membrane damage caused by colistin cannot grow on MacConkey agar containing bile salts.…”

Section: Resultsmentioning

confidence: 89%

“…Particularly, the frequent detection of mcr genes has been recognized in municipal wastewater (15,16). In Japan, mcr genes have so far been detected in both healthy and diseased food animals, in raw meat samples, including retail domestic chicken meat documented by us, and in human clinical samples, although their epidemiology has been sparsely studied (17)(18)(19). However, the occurrence of mcr genes in aquatic environments remains largely unknown in Japan, except for a very recent report on an E. coli sequence type 393 (ST393) strain carrying mcr-3.1 (20).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Acquisition of mcr-1 and Cocarriage of Virulence Genes in Avian Pathogenic Escherichia coli Isolates from Municipal Wastewater Influents in Japan

Hayashi

Tanaka

Taniguchi

et al. 2019

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

This study focused on the detection of the plasmid-mediated mcr colistin resistance gene in Escherichia coli isolates from wastewater treatment plants (WWTPs). Seven influent samples were collected from three WWTPs in Nagano Prefecture, Japan, during August and December 2018. Colistin-resistant E. coli isolates were selected on colistin-supplemented CHROMagar ECC plates. mcr-1-positive isolates were subjected to whole-genome sequencing (WGS) analysis. From six influent samples, seven mcr-1-positive but extended-spectrum β-lactamase (ESBL)-negative isolates belonging to different genetic lineages, namely, B2-O25:H4-ST131-fimH22, B2-O2:H1-ST135-fimH2, B1-O8:H9-ST764-fimH32, B1-O23:H16-ST453-fimH31, A-O81:H27-ST10-fimH54, A-O16:H5-ST871-fimH25, and F-O11:H6-ST457-fimH145, were detected. The MICs of colistin for these isolates ranged from 4 to 16 mg/liter. The mcr-1 genes were located on plasmids belonging to IncX4 and IncI2 in five and two isolates, respectively. Four IncX4 plasmids with the same size (33,309 bp) showed high sequence similarity (4 single-nucleotide variations). The remaining one IncX4 plasmid, with a size of 33,858 bp, carried the mcr-1 gene with the single synonymous nucleic substitution T27C. Two IncI2 plasmids with sizes of 60,710 bp and 60,733 bp had high sequence similarity (99.9% identity; 100% query coverage). Two of five isolates carrying IncX4 plasmids and both of the isolates carrying IncI2 plasmids harbored ColV plasmids carrying virulence-associated genes of avian pathogenic E. coli (APEC). In addition, another isolate of the B2-O25:H4-ST131-fimH22 lineage had those APEC-associated virulence genes on its chromosome. In conclusion, mcr-1-positive E. coli environmental isolates were mostly characterized as positive for APEC-associated virulence genes. The copresence of those genes may suggest the existence of a common source in animals and/or their associated environments. IMPORTANCE Colistin is considered a last-line therapeutic option in severe infections due to multidrug-resistant Gram-negative bacteria, in particular carbapenemase-producing Enterobacteriaceae and multidrug-resistant Acinetobacter baumannii. An increasing prevalence of mcr genes in diverse Enterobacteriaceae species, mainly Escherichia coli and Klebsiella pneumoniae from humans and food animals, has become a significant concern to public health all over the world. In Japan, mcr genes have so far been detected in food animals, raw meat, wastewater, and human clinical samples. This study reports the copresence of mcr-1 and avian pathogenic E. coli (APEC)-associated virulence genes in five of seven E. coli isolates recovered from aquatic environments in Japan. Our study highlights the importance and urgency of action to reduce environmental contamination by mcr genes that may likely occur due to exposure to untreated wastewater through combined sewer overflow by recent unusual weather.

show abstract

Section: Resultsmentioning

confidence: 89%

mentioning

confidence: 99%

Acquisition of mcr-1 and Cocarriage of Virulence Genes in Avian Pathogenic Escherichia coli Isolates from Municipal Wastewater Influents in Japan

Hayashi

Tanaka

Taniguchi

et al. 2019

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Unabsorbed colistin in a pig is excreted from the pig's gastrointestinal tract into the environment, and this could induce colistin resistance among bacteria that reside in the environment by various colistin resistance mechanisms similar to the bacteria in the gastrointestinal tracts of pigs that received colistin. Colistin-resistant Enterobacteriaceae that emerge in pigs receiving colistin can be transmitted to other pigs that do not receive colistin, and this would contaminate pork that is consumed by human beings, and potentially contaminate the environment [26][27][28][29][30]. Moreover, the plasmid-mediated mcr-1 gene could potentially be transferred horizontally from colistin-resistant Enterobacteriaceae to other species of bacteria and become resistant to colistin [6].…”

Section: Plos Onementioning

confidence: 99%

Association between the use of colistin for short-term treatment of Gram-negative bacterial infections and the emergence of colistin-resistant Enterobacteriaceae in swine from selected swine farms in Thailand

et al. 2020

View full text Add to dashboard Cite

Long-term use of colistin for preventing Gram-negative bacterial infections in food animals was prohibited in Thailand in 2017, but it is permitted for short-term treatment. This study aimed to investigate association between the use of colistin for short-term treatment of infection and the emergence of colistin-resistant Enterobacteriaceae in swine. The current study was conducted at 2 selected swine farms in Thailand. Neither farm has used colistin to prevent infection for longer than 1 year. Rectal swabs were collected from the same 66 pigs at birth, and on days 7, 14, 21, 28, and 60. Colistin was used to treat sick pigs for up to 3 days. Additional rectal swabs were collected during colistin treatment. Rectal swabs were analyzed for colistin-resistant Enterobacteriaceae and the mcr-1 gene. Results revealed that colistin-resistant Enterobacteriaceae were absent at birth. Some pigs at both farms had diarrhea and received colistin treatment during days 2-27. Colistin-resistant Enterobacteriaceae were detected in 13.3-50.0% of sick and healthy pigs. No sick pigs were observed during days 28-60, and colistin was not used during that period. Colistin-resistant Enterobacteriaceae were detected in 2.8-10.0% of healthy pigs on day 28, and in 0-3.4% of healthy pigs on day 60. The mcr-1 gene was detected in 57.6% of colistin-resistant Enterobacteriaceae isolates. Short-term treatment with colistin was found to be associated with the emergence of colistin-resistant Enterobacteriaceae in swine. Colistin-resistant Enterobacteriaceae rapidly emerged after colistin use, and rapidly decreased or disappeared after its discontinuation.

show abstract

“…For instance, a study showed that the prevalence of mcr-1-harboring E. coli in chicken meat samples collected from the Netherlands between 2009 and 2014 was 1.5% [20], whereas another study on chicken meat samples collected from the same country in 2015 showed a prevalence of 24.8% [21]. Similarly, other studies have shown that the detection rate of E. coli possessing mcr-1 from chicken meat samples collected from Germany [19], China [2], Japan [22], Brazil [23], and Nepal [24] ranged from 1.4-19.5%. More recently, a study in Tunisia showed that the rate of E. coli possessing mcr-1 from a chicken meat sample was relatively high, at 38.3% [25].…”

Section: Discussionmentioning

confidence: 97%

Quantitative Analysis of Colistin-Resistant Escherichia Coli in Retail Meat from Local Vietnamese Markets

Nguyen

Khong

et al. 2020

Preprint

View full text Add to dashboard Cite

Objective: The spread of drug-resistant bacteria via food has contributed to the dissemination of resistant bacteria among humans. However, the status of food contamination with resistant bacteria, particularly the level of resistant bacteria in food, have not yet been well elucidated.Results: In this study, the abundance of colistin-resistant Escherichia coli in meat samples was quantified to understand the origin of the contamination of meat available in local Vietnamese markets. Fifteen samples each of chicken and pork meat purchased from local Vietnamese markets were assessed for the presence of colistin-resistant E. coli with the mobile colistin resistance gene mcr. The results showed that 40% (6/15) and 66% (10/15) of the pork and chicken meat samples, respectively, were contaminated with colistin-resistant E. coli. The median levels of colistin-resistant E. coli in the contaminated pork and chicken samples were 1.8 × 104 and 4.2 × 103 CFU/g, respectively. The results of phylogenetic analysis of isolates from a chicken meat sample showed that the contaminated colistin-resistant E. coli were a mix of multiple phylogenetical clones of bacteria that may have multiplied during sale. This is the first study to quantify the abundance of colistin-resistant E. coli in meat samples.

show abstract

First Detection of an <i>Escherichia coli</i> Strain Harboring the <i>mcr-1</i> Gene in Retail Domestic Chicken Meat in Japan

Cited by 26 publications

References 16 publications

Acquisition of mcr-1 and Cocarriage of Virulence Genes in Avian Pathogenic Escherichia coli Isolates from Municipal Wastewater Influents in Japan

Acquisition of mcr-1 and Cocarriage of Virulence Genes in Avian Pathogenic Escherichia coli Isolates from Municipal Wastewater Influents in Japan

Association between the use of colistin for short-term treatment of Gram-negative bacterial infections and the emergence of colistin-resistant Enterobacteriaceae in swine from selected swine farms in Thailand

Quantitative Analysis of Colistin-Resistant Escherichia Coli in Retail Meat from Local Vietnamese Markets

Contact Info

Product

Resources

About