Co-localised Raman and force spectroscopy reveal the roles of hydrogen bonds and π-π interactions in defining the mechanical properties of diphenylalanine nano- and micro-tubes

Sinjab, Faris; Bondakov, Georgi; Notingher, Ioan

doi:10.1063/1.4885090

Cited by 4 publications

(5 citation statements)

References 26 publications

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“…Lekprasert et al used polarized Raman spectroscopy in combination with atomic force microscopy to study diphenylalanine nano-and micro-tube [138]. The authors were able to determine the orientation of molecules within the structure [139] and show that the structure remained constant when force was applied using an atomic force microscope [140].…”

Section: Polarized Raman Spectroscopymentioning

confidence: 99%

“…Reprinted (adapted) with permission. Copyright [140]. The molecules were found to maintain orientation under the application of force.…”

Section: Polarized Raman Spectroscopymentioning

confidence: 99%

“…Using data analysis techniques that will be discussed in Section 5, Raman spectroscopy has been used to classify bacteria [14,15], nano-bio-materials [138][139][140], cells [141,142], and animal and human tissues [143,144]. Because Raman spectroscopy is non-labeling and non-destructive, repeated measurements can be made of the same sample.…”

Section: Applicationsmentioning

confidence: 99%

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Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

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254

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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Section: Polarized Raman Spectroscopymentioning

confidence: 99%

“…Reprinted (adapted) with permission. Copyright [140]. The molecules were found to maintain orientation under the application of force.…”

Section: Polarized Raman Spectroscopymentioning

confidence: 99%

See 1 more Smart Citation

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

Self Cite

254

189

View full text Add to dashboard Cite

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“…Further control over the diameter and density of the PNTs is achieved by heating the FF solution prior to drop casting. While Raman spectroscopy is widely used in the chemical characterisation of materials, including PNTs, [26][27][28][29] a relatively large amount of analyte is necessary for Raman-based biosensing. Noble metals such as silver (Ag) can enhance the Raman signal, a method referred to as surface enhanced Raman scattering (SERS).…”

Section: Introductionmentioning

confidence: 99%

Wettability gradient-induced alignment of peptide nanotubes as templates for biosensing applications

et al. 2016

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“…Olive oil sample (referred in the paper as “oil”) was obtained from a local shop. The preparation of diphenylalanine (FF) microtubes is described elsewhere . The size of the prepared tubes ranged from a few to tens of micrometers in diameter.…”

Section: Methodsmentioning

confidence: 99%

Optical sectioning in multifoci Raman hyperspectral imaging

Liao

Sinjab

Elsheikha

et al. 2018

J Raman Spectroscopy

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In this study, we compared the depth discrimination and speed performance of multifoci Raman hyperspectral imaging with the reference standard of a single laser point confocal Raman mapping. A liquid crystal spatial light modulator was employed for the generation of multifoci laser beams, and a digital micromirror device was used as a software-configurable reflective pinhole array. The patterns of the laser foci and pinhole array can be rapidly changed without requiring any hardware alterations. Confocal patterns with different distance-to-size ratios were tested and compared. After optimization of the laser-foci pattern, we demonstrated the feasibility of multifoci Raman hyperspectral microscopy for recording depth-resolved molecular maps of biological cells (Acanthamoeba castellanii trophozoites). Micrometric depth discrimination and short acquisition times (20 min for single plane confocal image) were achieved. KEYWORDSconfocal Raman imaging, cross-talk, multi-beam, optical sectioning, single cell imaging | INTRODUCTIONRaman microspectroscopy (RMS) is a powerful technique for highly specific molecular imaging of samples in three dimensions (3D).[1] RMS instruments typically utilize a single laser beam to excite Raman scattering at a sample, which is analyzed by a spectrometer, with hyperspectral images formed by raster-scanning the laser spot and obtaining a Raman spectrum at each point. A pinhole is often placed at the spectrometer entrance to ensure confocal measurement configuration for improved depth discrimination. Although RMS allows sensitive and chemically specific hyperspectral imaging, acquisition times for measurements in 3D are often lengthy, due to the weak spontaneous Raman scattering cross section of many materials. The addition of a pinhole also typically comes at the cost of optical throughput for RMS detection, further increasing the acquisition times. Several techniques have been proposed and demonstrated for achieving fast Raman spectral mapping, such as line-or slit-scanning [2,3] and wide-field Raman imaging. [4,5] Compared with confocal single-beam scanning Raman imaging, these power-sharing Raman imaging techniques effectively shorten the mapping time by measuring multiple sample locations simultaneously. However, both line-scanning and wide-field techniques tend to decrease the depth discrimination because of decreased or lack of confocality. Multifoci excitation is a promising strategy to improve Raman imaging speed while maintaining good depth discrimination. [6][7][8][9][10][11][12] Raman spectra from these ------------------------------------------This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Co-localised Raman and force spectroscopy reveal the roles of hydrogen bonds and π-π interactions in defining the mechanical properties of diphenylalanine nano- and micro-tubes

Cited by 4 publications

References 26 publications

Raman spectroscopy: techniques and applications in the life sciences

Raman spectroscopy: techniques and applications in the life sciences

Wettability gradient-induced alignment of peptide nanotubes as templates for biosensing applications

Optical sectioning in multifoci Raman hyperspectral imaging

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