CO Laser Absorption Coefficients for Gases of Biological Relevance: H<sub>2</sub>O, CO<sub>2</sub>, Ethanol, Acetaldehyde, and Ethylene

Persijn, Stefan; Veltman, R.H.; Harren, F.J.M.; Parker, David H.

doi:10.1366/0003702001948123

Cited by 13 publications

(10 citation statements)

References 24 publications

(28 reference statements)

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“…Trace gases emitted by the plants in the sampling cell were transported by a gas flow to the photoacoustic detection cell situated within the laser cavity. The source of the infrared light was a CO laser that was line tunable over a large frequency range in the infrared wavelength region (200 laser lines between 5.0-and 7.7-mm wavelength; Persijn et al, 2000). From a spectroscopic point of view, the properties of .…”

Section: Lpad Of Trace Gasesmentioning

confidence: 99%

Laser Photoacoustic Detection Allows in Planta Detection of Nitric Oxide in Tobacco following Challenge with Avirulent and Virulent Pseudomonas syringae Pathovars

et al. 2005

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We demonstrate the use of laser photoacoustic detection (LPAD) as a highly sensitive method to detect in planta nitric oxide ( . NO) production from tobacco (Nicotiana tabacum . NO 1 O 3 / NO 2 1 O 2 ). The utility of the LPAD method was shown by examination of a nonhost hypersensitive response and a disease induced by Pseudomonas syringae (P. s.) pv phaseolicola and P. s. pv tabaci in tobacco.. NO was detected within 40 min of challenge with P. s. pv phaseolicola, some 5 h before the initiation of visible tissue collapse. The wildfire tobacco pathogen P. s. pv tabaci initiated. NO generation at 2 h postinfection. The use of . NO donors, the scavenger CPTIO ([4-carboxyphenyl]-4,5-dihydro-4,4,5,5-tetramethyl-3-oxide), and the mammalian nitric oxide synthase inhibitor L-NMMA (N G -monomethyl-L-arginine) indicated that . NO influenced the kinetics of cell death and resistance to both avirulent and virulent bacteria in tobacco. These observations suggest that .

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Section: Lpad Of Trace Gasesmentioning

confidence: 99%

Laser Photoacoustic Detection Allows in Planta Detection of Nitric Oxide in Tobacco following Challenge with Avirulent and Virulent Pseudomonas syringae Pathovars

et al. 2005

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“…12 In the overtone CO laser region ͑2.62-4.06 m͒ so far only a single attempt has been made to determine the concentration of gases. 10 With the present setup, and using a 75 cm long absorption cell extra cavity, absorption spectra for ethane, pentane, and ethylene have been determined.…”

Section: Laser Based Ethane Detectionmentioning

confidence: 99%

“…To eliminate the effect of interfering gases the carrier gas was led through a set of scrubbers and a cooling trap before entering the photoacoustic cell. 12 …”

Section: A Ethane Detection From Chilled Plantsmentioning

confidence: 99%

“…Therefore CO lasers in conjunction with photoacoustic cells have been employed to monitor trace gases. [7][8][9][10][11][12] The CO laser emission covers the spectral region from 4.6 to 8.2 m (vϭ1←2 to the vϭ35←36 band͒ providing intracavity laser powers up to 40 W. 7,8 To extend the number of accessible gases the wavelength region of the CO laser has been expanded ͑2.62-4.06 m͒ using the first overtone (⌬vϭ2) mode. Strong absorptions of hydrocarbons like methane, ethane, and pentane are found in this wavelength region.…”

Section: Introductionmentioning

confidence: 99%

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Laser-based trace gas detection of ethane as a result of photo-oxidative damage in chilled cucumber leaves (invited)

Santosa

Laarhoven

Harbinson

et al. 2003

Review of Scientific Instruments

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At low temperatures, high light intensity induces strong photooxidative lipid peroxidation in chilling sensitive cucumber leaves. A sensitive laser-based photoacoustic detector was employed to monitor on-line the evolution of ethane, one of the end products of lipid peroxidation. The ⌬vϭ2 CO laser operated in the 2.62-4.06 m infrared wavelength region with a maximum intracavity power of 11 W. In combination with an intracavity placed photoacoustic cell the laser was able to detect ethane down to 0.5 part per billion. Cucumber leaf disks chilled in the light produce ethane; the rate of ethane production depends on the applied temperature, light intensity, and period of chilling.

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“…In practice, interference between gases is normally present. Water vapor shows considerable absorption in some of the laser lines of interest (Persijn et al, 2000). As this absorption can strongly influence the detection of other compounds, it is recommendable to remove (as much as possible) water vapor from the gas mixture.…”

Section: The Photoacoustic Techniquementioning

confidence: 99%

Kinetics of Ethanol and Acetaldehyde Production in Fermenting Wheat Dough by Laser-Based Trace Gas Detection

Tomás

Harren

2001

Food sci. technol. int.

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The fermentation process of wheat dough was studied by laser-based trace gas detection. A CO-laser-driven photoacoustic spectrometer was used to monitor simultaneously, in real time, the ethanol and acetaldehyde production rates of two commercial types of active dry yeast (Saccharomyces cerevisiae) in wheat dough substrates. Both types of yeast, O and B, produced ethanol for 5 hours. Acetaldehyde was yielded only during 100 minutes by the yeast O, whereas the yeast B generated this compound for 3.5 hours. The total ethanol and acetaldehyde productions amounted to 1.50 mL/g dough and 4.5 µL/g dough in the O case, and 0.84 mL/g dough and 14.75 µL/g dough, in the B case, respectively. The yeasts were also tested for their ability to metabolize mixes consisting of the control wheat dough substrate and extra amounts of glucose or maltose. Both types of yeasts showed a similar ability to consume the added glucose, however, only the yeast B was found to possess an adapted enzymatic system to metabolize maltose. This work showed the efficiency of applying photoacoustic trace gas detection to food technology research for on-line monitoring of fermenting wheat dough.Se ha estudiado la fermentación de masa de trigo a través de la deteccion de trazas de gases mediante una técnica basada en el láser. Se empleó un espectrómetro fotoacústico CO-láser para la determinación simultánea en tiempo real de la producción de etanol y acetaldehído durante la fermentación con dos levaduras comerciales del género Saccharomyces cerevisiae. Ambos tipos de levaduras, denominadas O y B, produjeron etanol durante 5 horas. La levadura O produjo acetaldehído sólo durante 100 min, mientras que la B lo hizo durante 3,5 horas. La cantidad total de etanol y acetaldehído alcanzó con la levadura O 1,5 mL/g masa y 4,5 µL/g masa, repectivamente. En el caso de la levadura B fue de 0,84 mL/g masa para el etanol y 14,75 µL/g masa para el acetaldehído. También se probó la capacidad de las levaduras para metabolizar mezclas de la masa de trigo control con cantidades extra de glucosa o maltosa. Ambos tipos de levaduras mostraron una capacidad similar para consumir la glucosa añadida, sin embargo, sólo en la levadura B se detectó que poseía un sistema enzimático para metabolizar la matosa. En este trabajo se demuestra la eficiencia de aplicar la detección fotoacústica de trazas de gases para su uso en el control de a fermentación de masa de trigo.

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CO Laser Absorption Coefficients for Gases of Biological Relevance: H₂O, CO₂, Ethanol, Acetaldehyde, and Ethylene

Cited by 13 publications

References 24 publications

Laser Photoacoustic Detection Allows in Planta Detection of Nitric Oxide in Tobacco following Challenge with Avirulent and Virulent Pseudomonas syringae Pathovars

Laser Photoacoustic Detection Allows in Planta Detection of Nitric Oxide in Tobacco following Challenge with Avirulent and Virulent Pseudomonas syringae Pathovars

Laser-based trace gas detection of ethane as a result of photo-oxidative damage in chilled cucumber leaves (invited)

Kinetics of Ethanol and Acetaldehyde Production in Fermenting Wheat Dough by Laser-Based Trace Gas Detection

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CO Laser Absorption Coefficients for Gases of Biological Relevance: H2O, CO2, Ethanol, Acetaldehyde, and Ethylene

Cited by 13 publications

References 24 publications

Laser Photoacoustic Detection Allows in Planta Detection of Nitric Oxide in Tobacco following Challenge with Avirulent and Virulent Pseudomonas syringae Pathovars

Laser Photoacoustic Detection Allows in Planta Detection of Nitric Oxide in Tobacco following Challenge with Avirulent and Virulent Pseudomonas syringae Pathovars

Laser-based trace gas detection of ethane as a result of photo-oxidative damage in chilled cucumber leaves (invited)

Kinetics of Ethanol and Acetaldehyde Production in Fermenting Wheat Dough by Laser-Based Trace Gas Detection

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Resources

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CO Laser Absorption Coefficients for Gases of Biological Relevance: H₂O, CO₂, Ethanol, Acetaldehyde, and Ethylene