Co-inoculation of halotolerant potassium solubilizing Bacillus licheniformis and Aspergillus violaceofuscus improves tomato growth and potassium uptake in different soil types under salinity

Muthuraja, Raji; Muthukumar, Thangavelu

doi:10.1016/j.chemosphere.2022.133718

Cited by 21 publications

(18 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Wheat plants developed significantly longer shoots and roots, biomass and chlorophyll after inoculation with Aspergillus terreus BTK‐1, an endophytic fungus (Khan et al, 2022 ). Plants inoculated with Bacillus licheniformis and Aspergillus violaceofuscus improved in height, leaf area, biomass, relative water content, proline content and CAT activity (Muthuraja & Muthukumar, 2022 ). These indicated that Aspergillus has great potential in agricultural application.…”

Section: Discussionmentioning

confidence: 99%

New application of Aspergillus versicolor in promoting plant growth after suppressing sterigmatocystin production via genome mining and engineering

Zhao

Han

et al. 2022

Microbial Biotechnology

View full text Add to dashboard Cite

Aspergillus genus is a key component in fermentation and food processing. However, sterigmatocystin (STE)-a mycotoxin produced by several species of Aspergillus-limits the use of some Aspergillus species (such as Aspergillus versicolor, Aspergillus inflatus, and Aspergillus parasiticus) because of its toxicity and carcinogenicity. Here, we engineered an STE-free Aspergillus versicolor strain based on genome mining techniques. We sequenced and assembled the Aspergillus versicolor D5 genome (34.52 Mb), in which we identified 16 scaffolds and 54 biosynthetic gene clusters (BGCs). We silenced cytochrome P450 coding genes STC17 and STC27 by insertional inactivation. The production of STE in the Δstc17 mutant strain was increased by 282% but no STE was detected in the Δstc27 mutant. Metabolites of Δstc27 mutant exhibited growth-promoting effect on plants. Our study makes significant progress in improving the application of some Aspergillus strains by restricting their production of toxic and carcinogenic compounds.

show abstract

Section: Discussionmentioning

confidence: 99%

New application of Aspergillus versicolor in promoting plant growth after suppressing sterigmatocystin production via genome mining and engineering

Zhao

Han

et al. 2022

Microbial Biotechnology

View full text Add to dashboard Cite

show abstract

“…Both in vitro and in vivo experiments were conducted using previously characterized fungi (A. violaceofuscus-MH220545) and bacteria (B. licheniformis-MN718157) from saxicolous habitats (Muthuraja and Muthukumar, 2021a;Muthuraja and Muthukumar, 2021b;Muthuraja and Muthukumar, 2022).…”

Section: Biological Materialsmentioning

confidence: 99%

“…The experiment consisted of three replications for each concentration and different molecular weights of PEG. The experimental approach described in our previous study (Muthuraja and Muthukumar, 2022) was used for culture maintenance and microbial growth quantification. The proline content in KSM cultures was determined according to Kahraman and Karaderi (2021).…”

Section: Drought Tolerance Of Ksmsmentioning

confidence: 99%

“…The rice husk-based inoculum was prepared following the procedure mentioned in our earlier work (Muthuraja and Muthukumar, 2022). The rice husk used for the inoculum preparation was purchased from a local market.…”

Section: In Vivo Study 231 Preparation Of Inoculummentioning

confidence: 99%

“…Saxicolous (rock) microbial isolates have not been thoroughly studied for their role in improving the nutrient content of plants grown in different soil types under drought (Muthuraja and Muthukumar, 2021a). Further, the tolerance of KSMs like Aspergillus violaceofuscus and Bacillus licheniformis previously isolated from saxicolous habitats to drought stress is also unknown (Muthuraja and Muthukumar, 2022). Therefore, the present study aims to (i) assess the efficacy of A. violaceofuscus and B. licheniformis to tolerate drought stress induced by PEG of different molecular weights and concentrations under in vitro conditions; (ii) determine the influence of carrier-based formulation of the KSM isolates individually with/without mica supplement on tomato growth, chlorophyll content, and plant K content under drought conditions in different soil types, and (iii) explore the effect of co-inoculation of the above KSMs on the growth, RWC, antioxidant enzyme activities; and AM and DSE fungal root colonization in tomato plants under drought stress.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Drought tolerance of Aspergillus violaceofuscus and Bacillus licheniformis and their influence on tomato growth and potassium uptake in mica amended tropical soils under water-limiting conditions

Muthuraja

Muthukumar²,

Natthapol³

2023

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Drought is a significant abiotic stress that alters plant physiology and ultimately affects crop productivity. Among essential plant nutrients, potassium (K) is known to mitigate the deleterious effect of drought on plant growth. If so, K addition or inoculation of potassium solubilizing microorganisms (KSMs) that are tolerant to drought should promote plant growth during water stress. Therefore, in this study, K solubilizing Aspergillus violaceofuscus and Bacillus licheniformis, isolated from saxicolous environments, were tested for their capacity to tolerate drought using different molecular weights (~4000, 6000, and 8000 Da), and concentrations (0, 250, 500, 750, 1000, and 1250 mg/L) of polyethylene glycol (PEG) under in vitro conditions. The results showed that high concentrations (750 and 1000 mg/L) of PEG with different molecular weight considerably improved bacterial cell numbers/fungal biomass and catalase (CAT) and proline activities. Moreover, the ability of KSMs alone or in combination to impart drought tolerance and promote plant growth in the presence and absence of mica (9.3% K2O) supplementation was tested in Alfisol and Vertisol soil types under greenhouse conditions. The results revealed that the tomato plants inoculated with KSMs individually or dually with/without mica improved the physiological and morphological traits of the tomato plants under drought. Generally, tomato plants co-inoculated with KSMs and supplemented with mica were taller (2.62 and 3.38-fold) and had more leaf area (2.03 and 1.98-fold), total root length (3.26 and 8.86-fold), shoot biomass (3.87 and 3.93-fold), root biomass (9.00 and 7.24-fold), shoot K content (3.08 and 3.62-fold), root K content (3.39 and 2.03-fold), relative water content (1.51 and 1.27-fold), CAT activity (2.11 and 2.14-fold), proline content (3.41 and 3.28-fold), and total chlorophyll content (1.81 and 1.90-fold), in unsterilized Alfisol and Vertisol soil types, respectively, than uninoculated ones. Dual inoculation of the KSMs along with mica amendment, also improved the endorrhizal symbiosis of tomato plants more than their individual inoculation or application in both soil types. These findings imply that the A. violaceofuscus and B. licheniformis isolates are promising as novel bioinoculants for improving crop growth in water-stressed and rainfed areas of the tropics in the future.

show abstract

Enhancing Productivity Through Multiple Microbial Inoculants

Elmaghraby,

Abdellatif,

Amer

et al. 2023

Metabolomics, Proteomes and Gene Editing Approaches in Biofertilizer Industry

View full text Add to dashboard Cite

Co-inoculation of halotolerant potassium solubilizing Bacillus licheniformis and Aspergillus violaceofuscus improves tomato growth and potassium uptake in different soil types under salinity

Cited by 21 publications

References 65 publications

New application of Aspergillus versicolor in promoting plant growth after suppressing sterigmatocystin production via genome mining and engineering

New application of Aspergillus versicolor in promoting plant growth after suppressing sterigmatocystin production via genome mining and engineering

Drought tolerance of Aspergillus violaceofuscus and Bacillus licheniformis and their influence on tomato growth and potassium uptake in mica amended tropical soils under water-limiting conditions

Enhancing Productivity Through Multiple Microbial Inoculants

Contact Info

Product

Resources

About