CO in methanogenesis

Ferry, James G.

doi:10.1007/s13213-009-0008-5

Cited by 25 publications

(21 citation statements)

References 100 publications

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“…F 420 regeneration during growth on carbon monoxide. Both M. acetivorans and M. barkeri grow on carbon monoxide by oxidizing it to CO 2 and subsequently reducing CO 2 to methane (21). In methanogens, the reduction of carbon dioxide to methane requires the oxidation of two equivalents of reduced coenzyme F 420 .…”

Section: Model Reconstructionmentioning

confidence: 99%

“…Ech hydrogenase is not present in the M. acetivorans genome, and although an frh operon is present, it does not encode a functional enzyme (24). Thus, the mechanism for F 420 regeneration in M. acetivorans during growth on CO remains unknown (21).…”

Section: Model Reconstructionmentioning

confidence: 99%

“…This reaction, when coupled with methyl transfer to coenzyme M, would be strongly thermodynamically favored in the direction of methyl-CoM formation (⌬G 0 ϭ Ϫ30 kJ/mol) (67). The presence of the bypass reaction could permit tolerance for a wider range of environmental CO concentrations by permitting the cell to balance the increased ATP potential of Mtr with a possibly greater kinetic capacity of the Mtr bypass reaction (21).…”

Section: Model Reconstructionmentioning

confidence: 99%

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Genome-Scale Metabolic Reconstruction and Hypothesis Testing in the Methanogenic Archaeon Methanosarcina acetivorans C2A

et al. 2012

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Methanosarcina acetivorans strain C2A is a marine methanogenic archaeon notable for its substrate utilization, genetic tractability, and novel energy conservation mechanisms. To help probe the phenotypic implications of this organism's unique metabolism, we have constructed and manually curated a genome-scale metabolic model of M. acetivorans, iMB745, which accounts for 745 of the 4,540 predicted protein-coding genes (16%) in the M. acetivorans genome. The reconstruction effort has identified key knowledge gaps and differences in peripheral and central metabolism between methanogenic species. Using flux balance analysis, the model quantitatively predicts wild-type phenotypes and is 96% accurate in knockout lethality predictions compared to currently available experimental data. The model was used to probe the mechanisms and energetics of by-product formation and growth on carbon monoxide, as well as the nature of the reaction catalyzed by the soluble heterodisulfide reductase HdrABC in M. acetivorans. The genome-scale model provides quantitative and qualitative hypotheses that can be used to help iteratively guide additional experiments to further the state of knowledge about methanogenesis. Methanogenic archaea are unique in their ability to grow on low-energy substrates, such as acetic acid, by converting them into methane and other by-products. Methanogens are a critical part of the global carbon cycle, consuming by-products of other natural bioprocesses that would otherwise be recalcitrant in sulfate-poor, anaerobic environments (12). They also play an important role in global warming, since methane is a greenhouse gas 20 times as potent as carbon dioxide (42) and methanogenesis is the primary mechanism for the emission of methane into the atmosphere (2).Methanosarcina is the only known genus of methanogens with members that can utilize all of the known methanogenic pathways (acetoclastic, methylotrophic, hydrogenotrophic, and methyl reducing) (71). This metabolic diversity makes these species more permissive to metabolic and genetic manipulations than other methanogens. To capitalize on this characteristic, the genomes of three Methanosarcina species have been sequenced (15,22,38). In addition, genetic tools have been developed for several of these species, including methods for directed mutagenesis and regulated expression of specific genes (3,34,73,74).The constraint-based reconstruction and analysis (COBRA) strategy is a powerful paradigm for consolidating large amounts of metabolic knowledge and synthesizing that knowledge into quantitative phenotypic predictions (45, 51). For the performance of constraint-based analysis on an individual organism, its metabolic network is reconstructed from the bottom up, beginning with a sequenced and annotated genome and ending with a network of reactions and reaction-gene associations that directly link genotype and phenotype (68). Many metabolic reconstructions have been curated by hand and have been used to make useful predictions, such as the identification of put...

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Section: Model Reconstructionmentioning

confidence: 99%

Section: Model Reconstructionmentioning

confidence: 99%

Section: Model Reconstructionmentioning

confidence: 99%

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Genome-Scale Metabolic Reconstruction and Hypothesis Testing in the Methanogenic Archaeon Methanosarcina acetivorans C2A

et al. 2012

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“…Carbon monoxide (CO) has proven to be more inhibitory to methanogenesis than to hydrogenogenesis (i.e., H 2 -production), acetogenesis, and solventogenesis (i.e., acetate and alcohol production, respectively) at mesophilic and thermophilic temperatures (Ferry, 2010;Guiot, Cimpoia, & Carayon, 2011;Rother & Metcalf, 2004;Sipma et al, 2003). A few studies on this topic have reported enhancement of acetogenesis and inhibition of methanogenesis at CO partial pressures (P CO ) ≤ 0.3 atm (Alves et al, 2013;Guiot et al, 2011;Luo et al, 2013).…”

mentioning

confidence: 99%

Impact of carbon monoxide partial pressures on methanogenesis and medium chain fatty acids production during ethanol fermentation

Esquivel-Elizondo

Miceli

Torres

et al. 2017

Biotech & Bioengineering

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Medium-chain fatty acids (MCFA) are important biofuel precursors. Carbon monoxide (CO) is a sustainable electron and carbon donor for fatty acid elongation, since it is metabolized to MCFA precursors, it is toxic to most methanogens, and it is a waste product generated in the gasification of waste biomass. The main objective of this work was to determine if the inhibition of methanogenesis through the continuous addition of CO would lead to increased acetate or MCFA production during fermentation of ethanol. The effects of CO partial pressures (P ; 0.08-0.3 atm) on methanogenesis, fatty acids production, and the associated microbial communities were studied in batch cultures fed with CO and ethanol. Methanogenesis was partially inhibited at P ≥ 0.11 atm. This inhibition led to increased acetate production during the first phase of fermentation (0-19 days). However, a second addition of ethanol (day 19) triggered MCFA production only at P ≥ 0.11 atm, which probably occurred through the elongation of acetate with CO-derived ethanol and H :CO . Accordingly, during the second phase of fermentation (days 20-36), the distribution of electrons to acetate decreased at higher P , while electrons channeled to MCFA increased. Most probably, Acetobacterium, Clostridium, Pleomorphomonas, Oscillospira, and Blautia metabolized CO to H :CO , ethanol and/or fatty acids, while Peptostreptococcaceae, Lachnospiraceae, and other Clostridiales utilized these metabolites, along with the provided ethanol, for MCFA production. These results are important for biotechnological systems where fatty acids production are preferred over methanogenesis, such as in chain elongation systems and microbial fuel cells.

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“…In addition to these two well-known ways of producing methane, it can be produced similarly from substrates such as formic acid, methanol, methylamine or carbon monoxide [115][116][117][118]. It was long assumed that about 70% of total methane formed during biogas production stems from acetoclastic methanogenesis and the main prokaryotes responsible for this reaction are Methanosarcina spp.…”

Section: Microbiology and Biochemistry Of Cassava Biogas Productionmentioning

confidence: 99%

The potential of cassava biomass and applicable technologies for sustainable biogas production in South Africa: A review

Okudoh

Trois

Workneh

et al. 2014

Renewable and Sustainable Energy Reviews

118

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CO in methanogenesis

Cited by 25 publications

References 100 publications

Genome-Scale Metabolic Reconstruction and Hypothesis Testing in the Methanogenic Archaeon Methanosarcina acetivorans C2A

Genome-Scale Metabolic Reconstruction and Hypothesis Testing in the Methanogenic Archaeon Methanosarcina acetivorans C2A

Impact of carbon monoxide partial pressures on methanogenesis and medium chain fatty acids production during ethanol fermentation

The potential of cassava biomass and applicable technologies for sustainable biogas production in South Africa: A review

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