Co-expression of VpROMT gene from Chinese wild Vitis pseudoreticulata with VpSTS in tobacco plants and its effects on the accumulation of pterostilbene

Xu, Yan; Xu, Tao; Zhao, Xia; Zou, Yangjun; Li, Z. Q.; Jia, Xu; Li, F. J.; Wang, Y. J.

doi:10.1007/s00709-011-0335-9

Cited by 3 publications

(1 citation statement)

References 63 publications

(69 reference statements)

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“…A candidate gene approach was used, selecting an OMT sequence related to stilbene formation as probe, based on the close structural relationship between biphenyls and stilbenes. Major plant stilbenes are derivatives of resveratrol and pinosylvin, which typically occur in Vitis and Pinus species, respectively (Jorgensen, ; Chiron et al ., ; Schmidlin et al ., ; Xu et al ., ). Like biphenyls, pinosylvin derivatives lack substitution in ring B.…”

Section: Resultsmentioning

confidence: 97%

O‐Methyltransferases involved in biphenyl and dibenzofuran biosynthesis

et al. 2015

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SUMMARYBiphenyls and dibenzofurans are the phytoalexins of the Malinae involving apple and pear. Biosynthesis of the defence compounds includes two O-methylation reactions. cDNAs encoding the O-methyltransferase (OMT) enzymes were isolated from rowan (Sorbus aucuparia) cell cultures after treatment with an elicitor preparation from the scab-causing fungus, Venturia inaequalis. The preferred substrate for SaOMT1 was 3,5-dihydroxybiphenyl, supplied by the first pathway-specific enzyme, biphenyl synthase (BIS). 3,5-Dihydroxybiphenyl underwent a single methylation reaction in the presence of S-adenosyl-L-methionine (SAM). The second enzyme, SaOMT2, exhibited its highest affinity for noraucuparin, however the turnover rate was greater with 5-hydroxyferulic acid. Both substrates were only methylated at the meta-positioned hydroxyl group. The substrate specificities of the OMTs and the regiospecificities of their reactions were rationalized by homology modeling and substrate docking. Interaction of the substrates with SAM also took place at a position other than the sulfur group. Expression of SaOMT1, SaOMT2 and SaBIS3 was transiently induced in rowan cell cultures by the addition of the fungal elicitor. While the immediate SaOMT1 products were not detectable in elicitor-treated cell cultures, noraucuparin and noreriobofuran accumulated transiently, followed by increasing levels of the SaOMT2 products aucuparin and eriobofuran. SaOMT1, SaOMT2 and SaBIS3 were N-and C-terminally fused with the super cyan fluorescent protein and a modified yellow fluorescent protein, respectively. All the fluorescent reporter fusions were localized to the cytoplasm of Nicotiana benthamiana leaf epidermis cells. A revised biosynthetic pathway of biphenyls and dibenzofurans in the Malinae is presented.

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Section: Resultsmentioning

confidence: 97%

O‐Methyltransferases involved in biphenyl and dibenzofuran biosynthesis

et al. 2015

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Resveratrol and Its Derivatives as Phytoalexins

Stuart¹,

Robb²

2013

Bioactive Polyphenols From Wine Grapes

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Plant Tissue Culture: A Potential Tool for the Production of Secondary Metabolites

Garg,

Datta,

Ahmad

2024

In Vitro Propagation and Secondary Metabolite Production From Medicinal Plants: Current Trends (Part 2)

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Plants are an immense source of phytochemicals with therapeutic effects and are widely used as life-saving drugs, and other products of varied applications. Plant tissue culture is a unique technique employed under aseptic conditions from different plant parts called explants (leaves, stems, roots, meristems, etc.) for in vitro regeneration and multiplication of plants and synthesis of secondary metabolites (SMs). Selection of elite germplasm, high-producing cell lines, strain enhancements, and optimization of media and plant growth regulators may lead to increased in vitro biosynthesis of SMs. Interventions in plant biotechnology, like the synthesis of natural and recombinant bioactive molecules of commercial importance, have attracted attention over the past few decades; and the rate of SMs biosynthesis has increased manifold than the supply of intact plants, leading to a quick acceleration in its production through novel plant cultures. Over the years, the production of SMs in vitro has been enhanced by standardising cultural conditions, selection of high-yielding varieties, application of transformation methods, precursor feeding, and various immobilization techniques; however, most often, SM production is the result of abiotic or biotic stresses, triggered by elicitor molecules like natural polysaccharides (pectin and chitosan) that are used to immobilize and cause permeabilization of plant cells. In vitro synthesis of SMs is especially promising in plant species with poor root systems, difficulty in harvesting, unavailability of elite quality planting material, poor seed set and germination, and difficult to propagate species. Thus, the present article reviews various biotechnological interventions to enhance commercially precious SMs production in vitro.

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Co-expression of VpROMT gene from Chinese wild Vitis pseudoreticulata with VpSTS in tobacco plants and its effects on the accumulation of pterostilbene

Cited by 3 publications

References 63 publications

O‐Methyltransferases involved in biphenyl and dibenzofuran biosynthesis

O‐Methyltransferases involved in biphenyl and dibenzofuran biosynthesis

Resveratrol and Its Derivatives as Phytoalexins

Plant Tissue Culture: A Potential Tool for the Production of Secondary Metabolites

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