Co-encapsulation of dual drug loaded in MLNPs: Implication on sustained drug release and effectively inducing apoptosis in oral carcinoma cells

Mehnath, Sivaraj; Arjama, Mukherjee; Rajan, Mariappan; Annamalai, Govindhan; Jeyaraj, Murugaraj

doi:10.1016/j.biopha.2018.05.096

Cited by 35 publications

(24 citation statements)

References 42 publications

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“…Again, the significant reduction of the diameter of tumor cells in the third week is prevalent, especially those tumors treated with DNRloaded microcapsules. A great reduction in tumor volume was also reported by Mehnath et al (2018) [107]. Tumorinduced hamsters were orally given CaCO3 crystals coated with PDADMAC/PDCPP layers dualloaded with chrysin and cisplatin.…”

Section: Biological Applications Of Lmw Drug Loaded Pemcsmentioning

confidence: 87%

“…It is observed that the multilayer-coated crystals demonstrate a slower release rate (b) in comparison with the bare-CaCO3 microparticles (a), attributed to the hampered diffusion of the drug due to the additional multilayers. Moreover, a diffusion-mediated mechanism of release for PDADMAC/poly [di(sodium carboxyphenoxy)phosphazene] (PDCPP) coated CaCO3 nanoparticles was confirmed by Mehnath et al (2018) [107]. The coating possesses hydrophilic properties and undergoes hydrolytic degradation ( Figure 10); hence, pores form within the shell form and the matrix swells, leading to enhanced chrysin and cisplatin diffusion outwards.…”

Section: Effect Of Multilayers Upon the Kinetics Of Drug Releasementioning

confidence: 91%

“…Strong binding of small and large charged molecules to free (uncompensated by polyelectrolytes) changes can be evaluated by mobility of the laden molecules and the binding strongly affects the release kinetics [12,108,109]. Moreover, a diffusion-mediated mechanism of release for PDADMAC/poly [di(sodium carboxyphenoxy)phosphazene] (PDCPP) coated CaCO 3 nanoparticles was confirmed by Mehnath et al (2018) [107]. The coating possesses hydrophilic properties and undergoes hydrolytic degradation ( Figure 10); hence, pores form within the shell form and the matrix swells, leading to enhanced chrysin and cisplatin diffusion outwards.…”

Section: Effect Of Multilayers Upon the Kinetics Of Drug Releasementioning

confidence: 92%

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Encapsulation of Low-Molecular-Weight Drugs into Polymer Multilayer Capsules Templated on Vaterite CaCO3 Crystals

2020

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Polyelectrolyte multilayer capsules (PEMCs) templated onto biocompatible and easily degradable vaterite CaCO3 crystals via the layer-by-layer (LbL) polymer deposition process have served as multifunctional and tailor-made vehicles for advanced drug delivery. Since the last two decades, the PEMCs were utilized for effective encapsulation and controlled release of bioactive macromolecules (proteins, nucleic acids, etc.). However, their capacity to host low-molecular-weight (LMW) drugs (<1–2 kDa) has been demonstrated rather recently due to a limited retention ability of multilayers to small molecules. The safe and controlled delivery of LMW drugs plays a vital role for the treatment of cancers and other diseases, and, due to their tunable and inherent properties, PEMCs have shown to be good candidates for smart drug delivery. Herein, we summarize recent progress on the encapsulation of LMW drugs into PEMCs templated onto vaterite CaCO3 crystals. The drug loading and release mechanisms, advantages and limitations of the PEMCs as LMW drug carriers, as well as bio-applications of drug-laden capsules are discussed based upon the recent literature findings.

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Section: Biological Applications Of Lmw Drug Loaded Pemcsmentioning

confidence: 87%

Section: Effect Of Multilayers Upon the Kinetics Of Drug Releasementioning

confidence: 91%

Section: Effect Of Multilayers Upon the Kinetics Of Drug Releasementioning

confidence: 92%

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Encapsulation of Low-Molecular-Weight Drugs into Polymer Multilayer Capsules Templated on Vaterite CaCO3 Crystals

2020

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“…As discussed above, physical cross linking (intrinsic charge compensation) has an influence on pore size; this can be influenced by the orientation and the number of crosslinking groups of the polymers being used. Moreover, hydrophobicity is influential on the multilayer permeability [ 207 ], it is reported that the esterification of the polymers used increased the hydrophobic nature of the multilayer structure and thus decreased permeability [ 208 ]. This suggests that different polymer combinations and deposition conditions will inherently affect the multilayers’ ability of retaining bioactives.…”

Section: Problems Associated With Pemcsmentioning

confidence: 99%

Polyelectrolyte Multilayer Capsule (PEMC)-Based Scaffolds for Tissue Engineering

et al. 2020

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Tissue engineering (TE) is a highly multidisciplinary field that focuses on novel regenerative treatments and seeks to tackle problems relating to tissue growth both in vitro and in vivo. These issues currently involve the replacement and regeneration of defective tissues, as well as drug testing and other related bioapplications. The key approach in TE is to employ artificial structures (scaffolds) to support tissue development; these constructs should be capable of hosting, protecting and releasing bioactives that guide cellular behaviour. A straightforward approach to integrating bioactives into the scaffolds is discussed utilising polyelectrolyte multilayer capsules (PEMCs). Herein, this review illustrates the recent progress in the use of CaCO3 vaterite-templated PEMCs for the fabrication of functional scaffolds for TE applications, including bone TE as one of the main targets of PEMCs. Approaches for PEMC integration into scaffolds is addressed, taking into account the formulation, advantages, and disadvantages of such PEMCs, together with future perspectives of such architectures.

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“…Destruction of the pro‐ and anti‐apoptotic balance leads to interruption of apoptosis signaling in the cancer cells. It has been broadly proposed that regular overexpression of Bcl‐2 and downregulation of Bax expression in malignant environment is one of the fundamental mechanisms underlying apoptosis …”

Section: Introductionmentioning

confidence: 99%

A ginger derivative, zingerone—a phenolic compound—induces ROS‐mediated apoptosis in colon cancer cells (HCT‐116)

Veeraraghavan

Mohan

et al. 2019

J Biochem & Molecular Tox

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Zingerone (ZO), an active phenolic agent derived from Zingiber officinale (Ginger), has many pharmacological properties such as antioxidant, antiangiogenic, and antitumor. However, its potential value in cancer and the mechanism by which ZO wields its therapeutic effects remain obscure. Therefore, in this current study, we explored the effects of ZO on suppressing cell proliferation and enhancing apoptosis in colon cancer cells (HCT116). Our results indicated that ZO significantly enhances the production of reactive oxygen species, lipid peroxidation (thiobarbituric acid reactive substance [TBARS]), and loss of cell viability; and reduces mitochondrial membrane potential and antioxidant levels (SOD, CAT, and GSH) in ZO‐treated HCT116 cells in a dose‐dependent (2.5, 5, and 10 µM) manner. Furthermore, ZO induces oxidative stress‐mediated apoptosis as evidenced by apoptotic morphological changes predicted by AO/EtBr, Hoechst staining and further confirmed by comet assay. Moreover, immunoblotting techniques showed that ZO treatment effectively enhances Bax, caspase‐9, and caspase‐3 expressions and decreases the expression of Bcl‐2 in colon cancer cells. Together, our results evidenced that the antitumor effects of ZO reduce cell proliferation and stimulate apoptosis through modulating pro‐ and antiapoptotic molecular events in HCT116 colon cancer cells. Therefore, based on our findings, ZO may be used as a therapeutic agent for the treatment of colon cancer.

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Co-encapsulation of dual drug loaded in MLNPs: Implication on sustained drug release and effectively inducing apoptosis in oral carcinoma cells

Cited by 35 publications

References 42 publications

Encapsulation of Low-Molecular-Weight Drugs into Polymer Multilayer Capsules Templated on Vaterite CaCO3 Crystals

Encapsulation of Low-Molecular-Weight Drugs into Polymer Multilayer Capsules Templated on Vaterite CaCO3 Crystals

Polyelectrolyte Multilayer Capsule (PEMC)-Based Scaffolds for Tissue Engineering

A ginger derivative, zingerone—a phenolic compound—induces ROS‐mediated apoptosis in colon cancer cells (HCT‐116)

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