A ginger derivative, zingerone—a phenolic compound—induces ROS‐mediated apoptosis in colon cancer cells (HCT‐116)

Abstract

Zingerone (ZO), an active phenolic agent derived from Zingiber officinale (Ginger), has many pharmacological properties such as antioxidant, antiangiogenic, and antitumor. However, its potential value in cancer and the mechanism by which ZO wields its therapeutic effects remain obscure. Therefore, in this current study, we explored the effects of ZO on suppressing cell proliferation and enhancing apoptosis in colon cancer cells (HCT116). Our results indicated that ZO significantly enhances the production of reactive oxygen species, lipid peroxidation (thiobarbituric acid reactive substance [TBARS]), and loss of cell viability; and reduces mitochondrial membrane potential and antioxidant levels (SOD, CAT, and GSH) in ZO‐treated HCT116 cells in a dose‐dependent (2.5, 5, and 10 µM) manner. Furthermore, ZO induces oxidative stress‐mediated apoptosis as evidenced by apoptotic morphological changes predicted by AO/EtBr, Hoechst staining and further confirmed by comet assay. Moreover, immunoblotting techniques showed that ZO treatment effectively enhances Bax, caspase‐9, and caspase‐3 expressions and decreases the expression of Bcl‐2 in colon cancer cells. Together, our results evidenced that the antitumor effects of ZO reduce cell proliferation and stimulate apoptosis through modulating pro‐ and antiapoptotic molecular events in HCT116 colon cancer cells. Therefore, based on our findings, ZO may be used as a therapeutic agent for the treatment of colon cancer.

Zingiberis Rhizoma Recens: A Review of Its Traditional Uses, Phytochemistry, Pharmacology, and Toxicology

“…6-Shogaol has been demonstrated to have potential anticancer activities towards human renal-cell carcinoma 786-O [ 76 ] and ovarian cancer cell lines A2780 [ 77 ], and head and neck squamous cell carcinoma cell lines [ 78 ]. Studies showed that 6-shogaol had a dose-dependent antiproliferative effect and induced the apoptosis of head and neck squamous cell carcinoma cell lines by downregulating survivin [ 78 ].…”

“…Studies showed that 6-shogaol had a dose-dependent antiproliferative effect and induced the apoptosis of head and neck squamous cell carcinoma cell lines by downregulating survivin [ 78 ]. 6-Gingerol inhibited STAT-3 translocation of ovarian cancer cells to inhibit cell proliferation and induce apoptosis [ 77 ].…”

Effects Of Dentifrices On Surface Properties Of Cad - Cam Manufactured Temporary Restorative Materials

“…A wide variety of pre-polymerised polymethyl methacrylate (PMMA) blocks have been introduced on the market based on further studies related with nanoparticles and data was produced on surface roughness [11]. Our team has extensive knowledge and research experience that has translated into high quality publications [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31].…”

Ginger (Zingiber officinale) and its Bioactive Components with Protective and Therapeutic Potential against Cancer

“…The eff ects of zingerone on suppressing cell proliferation and enhancing apoptosis in colon cancer cells (HCT116) was explored by Su, et al [30]. The results indicated that zingerone signifi cantly enhances the production of reactive oxygen species, lipid peroxidation (Thiobarbituric Acid Reactive Substance [TBARS]), and loss of cell viability; and reduces mitochondrial membrane potential and antioxidant levels (SOD, CAT, and GSH) in ZO-treated HCT116 cells in a dose-dependent (2.5, 5, and 10 μM) manner.…”