Co-delivery of curcumin and Bcl-2 siRNA by PAMAM dendrimers for enhancement of the therapeutic efficacy in HeLa cancer cells

Ghaffari, Maryam; Dehghan, Gholamreza; Baradaran, Behzad; Zarebkohan, Amir; Mansoori, Behzad; Soleymani, Jafar; Dolatabadi, Jafar Ezzati Nazhad; Hamblin, Michael R.

doi:10.1016/j.colsurfb.2019.110762

Cited by 113 publications

(57 citation statements)

References 46 publications

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“…Ghaffari and co-workers developed polyamidoamine (PAMAM)-based dendrimers for the co-delivery of curcumin and Bcl-2 siRNA against HeLa cancer cells [109]. The particle size analysis of co-encapsulated polymeric dendrimers was 180 nm with a zeta potential of −48 mV.…”

Section: Dendrimersmentioning

confidence: 99%

“…The dendrimers co-loaded with curcumin and Bcl-2 siRNA exhibited in vitro HeLa cell growth inhibition in a time-and concentration-dependent manner. The co-delivery of curcumin and Bcl-2 siRNA resulted in enhanced cellular drug uptake [109].…”

Section: Dendrimersmentioning

confidence: 99%

“…- [109] Hydrogel Cyclodextrin Curcumin and resveratrol MCF-7 Breast cancer A cytotoxic synergistic effect on the MCF-7 breast cancer cells with a combination index value of 0.29 [113] Nanocapsules Poly (allyl amine) hydrochloride…”

Section: Exosomesmentioning

confidence: 99%

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Efficacy of Polymer-Based Nanocarriers for Co-Delivery of Curcumin and Selected Anticancer Drugs

Alven

Aderibigbe

2020

Nanomaterials

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Cancer remains a heavy health burden resulting in a high rate of mortality around the world. The presently used anticancer drugs suffer from several shortcomings, such as drug toxicity, poor biodegradability and bioavailability, and poor water solubility and drug resistance. Cancer is treated effectively by combination therapy whereby two or more anticancer drugs are employed. Most of the combination chemotherapies result in a synergistic effect and overcome drug resistance. Furthermore, the design of polymer-based nanocarriers for combination therapy has been reported by several researchers to result in promising therapeutic outcomes in cancer treatment. Curcumin exhibits good anticancer activity but its poor bioavailability has resulted in its incorporation into several polymer-based nanocarriers resulting in good biological outcomes. Furthermore, the incorporation of curcumin together with other anticancer drugs have been reported to result in excellent therapeutic outcomes in vivo and in vitro. Due to the potential of polymer-based nanocarriers, this review article will be focused on the design of polymer-based nanocarriers loaded with curcumin together with other anticancer drugs.

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Section: Dendrimersmentioning

confidence: 99%

Section: Dendrimersmentioning

confidence: 99%

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Efficacy of Polymer-Based Nanocarriers for Co-Delivery of Curcumin and Selected Anticancer Drugs

Alven

Aderibigbe

2020

Nanomaterials

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“…HeLa cells were cultured in RPMI-1640 containing antibiotics (100 units/mL penicillin and 50 units/mL streptomycin) and fetal bovine serum (10%), in an atmosphere with a relative humidity of 95% and CO 2 concentration of 5%. The cytotoxic effects of different formulations against HeLa cancer cells was measured by MTT assay [20]. The cells were seeded in 96-well plates (1 Â 10 4 cells/well) in 200 mL medium and subsequently cultured overnight.…”

Section: Cell Proliferation Assaymentioning

confidence: 99%

Design and syntheses of 7-nitro-2-aryl-4H-benzo[d][1,3]oxazin-4-ones as potent anticancer and antioxidant agents

Bari

Khan

Shahzad

et al. 2020

Journal of Molecular Structure

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a b s t r a c tA group of new nitro substituted benzoxazinones (3a-k) were synthesized from easily available 4nitroanthranilic acid. All the synthesized compounds were characterized by FT-IR, 1 H NMR, 13 C NMR, mass spectrometry and elemental analysis. Anti-proliferative and pro-apoptotic potential of all the synthesized compounds (3a-k) was evaluated by MTT and Hoechst 33258 staining assay respectively whereas their antioxidant properties were determined via DPPH free radical scavenging assay. The most active compounds (3a, 3c and 3k) showed significant cytotoxic potential against HeLa cells with an inhibition of cell viability that ranged between 28.54 and 44.67% (P < 0.001). Albeit statistically different, the anti-proliferative effect of 3c was in close match with that of the reference drug doxorubicin. Likewise, the test compounds showed profound pro-apoptotic potential with an apoptotic index that ranged between 52.86 and 75.61%. Besides, the docking studies revealed a higher efficiency for compounds (3a and 3h) owing to their better affinity and inhibition constant (K i ¼ 4.397 and 3.713 nmol) respectively. The antioxidant potential of synthesized benzoxazinones (3a-k) was in close agreement with the experimental anticancer results with a percent inhibition from 34.45 to 85.93% as compared to standard (90.56%).

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“…Therefore, the underlying mechanisms of anti-cancer activity of Cur has recently been studied [8,9]. Evidence suggests that Cur has an inhibitory effect on proliferation of cancer cells through targeting several cancer-related pathways, such as apoptosis and cell cycle [10].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Anticancer Potency of Hydroxytyrosol and Curcumin by PLGA-PAA Nano-Encapsulation on PANC-1 Pancreatic Cancer Cell Line

Farhoudi

Shadman

Chavoshi

et al. 2020

Preprint

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Background: Pancreatic cancer (PC) is a highly aggressive malignancy associated with low survival rates. Many chemotherapeutic regimens have been investigated for advanced unresectable and metastatic PC, but with only minimal improvement in survival and prognosis. The present study aimed to investigate the anti-cancer function of free and nano-encapsulated hydroxytyrosol (Hyd) and curcumin (Cur), and its combinations (Hyd-Cur) on the PANC-1 cell line.Methods: The poly lactide-co-glycolide-co-polyacrylic acid (PLGA-co-PAA) nano-encapsulated Hyd and Cur were synthesized, and MTT assay was performed to evaluate cytotoxic effects of free and nano-encapsulated Hyd, Cur, and Hyd-Cur. Moreover, effects of free and nano-encapsulated Hyd, Cur, and Hyd-Cur were evaluated on viability, migration, morphological alterations, colony formation, and apoptosis on PANC-1 cell line. The mRNA expression levels of MMP2, MMP9, BAX, BCL-2, and Cas9 genes were assessed after treated PANC-1 cells with free and nano-encapsulated Hyd, Cur, and Hyd-Cur.Results: The obtained results showed that free and nano-encapsulated Hyd, Cur, and Hyd-Cur treatments significantly decreased the viability, migration, and colony formation in the PANC-1 cells. Furthermore, apoptosis rates in PANC-1 cells were increased in a concentration and time dependent manner in all of the treatment groups. Moreover, anti-proliferative activity of nano-encapsulated Hyd-Cur was significantly more than other treatments.Conclusions: According to our results, Hyd-Cur combination and nano-encapsulation therapy exerts more profound apoptotic and anti-proliferative effects on PANC-1 cell line than free Hyd or Hyd monotherapy.

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Co-delivery of curcumin and Bcl-2 siRNA by PAMAM dendrimers for enhancement of the therapeutic efficacy in HeLa cancer cells

Cited by 113 publications

References 46 publications

Efficacy of Polymer-Based Nanocarriers for Co-Delivery of Curcumin and Selected Anticancer Drugs

Efficacy of Polymer-Based Nanocarriers for Co-Delivery of Curcumin and Selected Anticancer Drugs

Design and syntheses of 7-nitro-2-aryl-4H-benzo[d][1,3]oxazin-4-ones as potent anticancer and antioxidant agents

Enhanced Anticancer Potency of Hydroxytyrosol and Curcumin by PLGA-PAA Nano-Encapsulation on PANC-1 Pancreatic Cancer Cell Line

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