Co-culturing Bacillus subtilis and wastewater microbial community in a bio-electrochemical system enhances denitrification and butyrate formation

Rahimi, Shadi; Modin, Oskar; Roshanzamir, Fariba; Neissi, Alireza; Saheb-Alam, Soroush; Seelbinder, Bastian; Pandit, Santosh; Shi, Lei; Mijaković, Ivan

doi:10.1016/j.cej.2020.125437

Cited by 27 publications

(10 citation statements)

References 64 publications

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“…There are several methods of nitrate removal like reverse osmosis, electrochemical reduction, ion exchange and electrodialysis, which are expensive. The use of conventional methods like bioaugmentation of wastewater with microbial agents in combination with the bioelectrochemical process speeds up denitrification (Rahimi et al, 2020). This can be used for the commercial production of butyrate from carbohydrate‐containing wastewater like dairy farm discharges.…”

Section: Improvement Of Soil Qualitymentioning

confidence: 99%

Bacillus subtilisimpact on plant growth, soil health and environment: Dr. Jekyll and Mr. Hyde

Mahapatra

Yadav

Ramakrishna

2022

Journal of Applied Microbiology

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The increased dependence of farmers on chemical fertilizers poses a risk to soil fertility and ecosystem stability. Plant growth‐promoting rhizobacteria (PGPR) are at the forefront of sustainable agriculture, providing multiple benefits for the enhancement of crop production and soil health. Bacillus subtilis is a common PGPR in soil that plays a key role in conferring biotic and abiotic stress tolerance to plants by induced systemic resistance (ISR), biofilm formation and lipopeptide production. As a part of bioremediating technologies, Bacillus spp. can purify metal contaminated soil. It acts as a potent denitrifying agent in agroecosystems while improving the carbon sequestration process when applied in a regulated concentration. Although it harbours several antibiotic resistance genes (ARGs), it can reduce the horizontal transfer of ARGs during manure composting by modifying the genetic makeup of existing microbiota. In some instances, it affects the beneficial microbes of the rhizosphere. External inoculation of B. subtilis has both positive and negative impacts on the endophytic and semi‐synthetic microbial community. Soil texture, type, pH and bacterial concentration play a crucial role in the regulation of all these processes. Soil amendments and microbial consortia of Bacillus produced by microbial engineering could be used to lessen the negative effect on soil microbial diversity. The complex plant–microbe interactions could be decoded using transcriptomics, proteomics, metabolomics and epigenomics strategies which would be beneficial for both crop productivity and the well‐being of soil microbiota. Bacillus subtilis has more positive attributes similar to the character of Dr. Jekyll and some negative attributes on plant growth, soil health and the environment akin to the character of Mr. Hyde.

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Section: Improvement Of Soil Qualitymentioning

confidence: 99%

Bacillus subtilisimpact on plant growth, soil health and environment: Dr. Jekyll and Mr. Hyde

Mahapatra

Yadav

Ramakrishna

2022

Journal of Applied Microbiology

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“…cDNA was synthesized from the isolated RNA. The expression of AMO and NXR genes was analyzed by q-PCR according to Rahimi et al 2020 [29]. The relative values of gene expression were assessed using Agilent Technologies Stratagene Mx30005P and were calculated according to the manufacturer's instructions [21].…”

Section: Quantitative Pcr (Q-pcr)mentioning

confidence: 99%

Enriched Microbial Communities for Ammonium and Nitrite Removal from Recirculating Aquaculture Systems

Neissi¹,

Rafiee²,

Rahimi³

et al. 2021

Preprint

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Background: The aim of this study was the enrichment of high-performance microbial communities in biofilters for removal of ammonium and nitrite from aquaculture water. Methods: Ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) were enriched from different environmental water samples. The microbial communities with higher ammonium and nitrite removal activity were selected and adapted to different temperatures [9 ºC, 15 ºC, room temperature (25 ºC), and 30 ºC]. The expression of genes involved in nitrification including ammonia monooxygenase (AMO) and nitrite oxidoreductase (NXR) were measured in temperature-adapted AOB and NOB microbiomes. The microbial species present in the selected microbiomes were identified via 16s rRNA sequencing. Results: The microbial communities containing Nitrosomonas oligotropha and Nitrobacter winogradskyi showed the highest ammonium and nitrite removal activity at all temperatures used for adaptation. Furthermore, the microbial communities do not contain any pathogenic bacteria. They also exhibited the highest expression of AMO and NXR genes. Using the enriched microbial communities, we achieved a 288% and 181% improvement in ammonium and nitrite removal over the commonly used communities in biofilters at 9 °C, respectively. Conclusions: These results suggest that the selected microbiomes allowed for a significant improvement of water quality in a recirculating aquaculture system (RAS).

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“…This con guration of bacterial growth in the 3 layers would make the same bacteria act as nitrate reducing, azo dye reducing and butyrate producing, rendering the bio lm granules as a complete system suitable for textile wastewater bioremediation and ensure longevity of performance. Butyrate was produced by Bacillus subtilis under anaerobic fermentation (Rahimi et al 2020). The spherical architecture proposed has been previously stated as an acceptable conceptual model for aggregates used in bioremediation under different oxygen levels (Aqeel et al 2019).…”

Section: Biophysical Changes Of Bacillus Sp In the Presence Of Different Nitrate Concentrationsmentioning

confidence: 99%

Nitrate modulation of Bacillus sp. biofilm components: a proposed model for sustainable bioremediation

2021

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The presence of different pollutants in wastewater hinder microbial growth, compromise enzymatic activity or compete for electrons required for bioremediation pathway. Therefore, there is a need to use a single microorganism that is capable of tolerating different toxic compounds and can perform simultaneous bioremediation. In the present study, nitrate reducing bacteria capable of decolorizing azo dye was identi ed as Bacillus subtillis sp. DN using Protein pro ling, morphological and biochemical tests X-ray diffraction pattern, Raman spectroscopy and cyclic voltammetry con rm that the bacterium under study possesses membrane-bound nitrate reductase and that is capable of direct electron transfer. The addition of nitrate concentrations (0-50 mM) resulted in increased bio lm formation with variable exopolysaccharides (EPS), protein, and eDNA. Fourier Transform Infrared spectrum revealed the presence of a biopolymer at high nitrate concentrations. Effective capacitance and conductivity of the cells grown in different nitrate concentrations suggest changes in the relative position of polar groups, their relative orientation and permeability of cell membrane as detected by dielectric spectroscopy. The increase in bio lm shifted the removal of the azo dye from biodegradation to bioadsorption. Our results indicate that nitrate modulates bio lm components. Bacillus sp. DN granular bio lm can be used for simultaneous nitrate and azo dye removal from wastewater.

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Co-culturing Bacillus subtilis and wastewater microbial community in a bio-electrochemical system enhances denitrification and butyrate formation

Cited by 27 publications

References 64 publications

Bacillus subtilisimpact on plant growth, soil health and environment: Dr. Jekyll and Mr. Hyde

Bacillus subtilisimpact on plant growth, soil health and environment: Dr. Jekyll and Mr. Hyde

Enriched Microbial Communities for Ammonium and Nitrite Removal from Recirculating Aquaculture Systems

Nitrate modulation of Bacillus sp. biofilm components: a proposed model for sustainable bioremediation

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