Co-culture with Sertoli cells promotes proliferation and migration of umbilical cord mesenchymal stem cells

Zhang, Fenxi; Hong, Yan; Wenmei, Liang; Ren, Tongming; Jing, Suhua; Lin, Juntang

doi:10.1016/j.bbrc.2012.09.007

Cited by 27 publications

(15 citation statements)

References 20 publications

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“…Immunofluorescence staining showed that bmMSCs were positive for CD44 and CD90, which are MSC specific markers [1,20]. It is known that bmMSCs have the potential to differentiate to osteoblasts and apidocytes.…”

Section: Resultsmentioning

confidence: 99%

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TGF-β1 induces senescence of bone marrow mesenchymal stem cells via increase of mitochondrial ROS production

Niu

et al. 2014

BMC Dev Biol

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BackgroundBone marrow derived mesenchymal stem cells (bmMSCs) are multipotent cells that can differentiate into diverse cell types, including cardiomyocytes. BmMSC-based transplantation is capable of repairing acute and chronic myocardial infarction. Prior to the transplantation, MSCs are usually induced in vitro by biological reagents and chemicals for directional differentiation. Transforming growth factor beta (TGF-β) is one of the most commonly used biological reagents for induction of cardiomyocyte differentiation of bmMSCs. Previous studies have shown that TGF-β induces senescence in several cell types. However, whether TGF-β affects senescence of bmMSCs has not been elucidated. The goal of this study was to investigate the effect of TGF-β1 on senescence of bmMSCs and the underlying mechanisms.ResultsWe found that TGF-β1 increased activity of senescence-associated-galactosidase (SA-Gal) and production of mitochondrial reactive oxygen species (mtROS) in bmMSCs in a dose-dependent manner. TGF-β1 also significantly decreased expression of superoxide dismutase 2 (SOD2) and Id1, and increased expression of 4-Hydroxynonenal (4-HNE) subunits and p16 in bmMSCs in a dose-dependent manner. Pre-treatment with mtROS inhibitor acetyl-L-carnitine (ALCAR, 0.1 mM) significantly inhibited TGF-β1-induced mtROS production and SA-Gal activity.ConclusionTGF-β1 can induce senescence of bmMSCs, which at least partially depends on mtROS production.

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Section: Resultsmentioning

confidence: 99%

“…Mesenchymal stem cells (MSCs) are multipotent adult stem cells with a high capacity for self-renewal and capable of differentiating into a variety of cell types, including adipocytes, osteoblasts, chondrocytes, endothelial cells, cardiomyocytes and neurons [1,2]. Currently, MSCs have been widely used in regenerative medicine [3].…”

Section: Introductionmentioning

confidence: 99%

TGF-β1 induces senescence of bone marrow mesenchymal stem cells via increase of mitochondrial ROS production

Niu

et al. 2014

BMC Dev Biol

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“…Hung et al demonstrated that short-term exposure of MSCs to hypoxia leads to increased expression of chemokine receptors (CX3CR1 and CXCR4) that may aid engraftment in vivo [81]. A similar increase in chemokine receptor (CXCR4) apart from cell proliferation-associated cyclin (cyclin D1, D3) expression was observed when humans umbilical cord MSCs were cocultured with Sertoli cells [82]. Yet another approach is the use of genetically engineered MSCs that overexpress chemokine receptors such as CXCR4 and integrin-alpha4 to influence their homing ability.…”

Section: Approaches To Improve Homingmentioning

confidence: 99%

Mesenchymal Stem Cells Migration Homing and Tracking

Sohni

Verfaillie

2013

Stem Cells International

350

291

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“…Another study done by Zhang et al . () indicated that indirect contact between HUVECs and Sertoli cells in Transwell chambers enhanced the proliferation and migration of HUVECs in vitro via upregulation of genes involved in the regulation of cell cycle progression.…”

Section: Relevant Cues From Supporting Cells In Directing Hamcs Fatementioning

confidence: 99%

Microenvironmental factors involved in human amnion mesenchymal stem cells fate decisions

Syva

Ampon

Lasimbang

et al. 2015

J Tissue Eng Regen Med

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Human amnion mesenchymal stem cells (HAMCs) show great differentiation and proliferation potential and also other remarkable features that could serve as an outstanding alternative source of stem cells in regenerative medicine. Recent reports have demonstrated various kinds of effective artificial niche that mimic the microenvironment of different types of stem cell to maintain and control their fate and function. The components of the stem cell microenvironment consist mainly of soluble and insoluble factors responsible for regulating stem cell differentiation and self-renewal. Extensive studies have been made on regulating HAMCs differentiation into specific phenotypes; however, the understanding of relevant factors in directing stem cell fate decisions in HAMCs remain underexplored. In this review, we have therefore identified soluble and insoluble factors, including mechanical stimuli and cues from the other supporting cells that are involved in directing HAMCs fate decisions. In order to strengthen the significance of understanding on the relevant factors involved in stem cell fate decisions, recent technologies developed to specifically mimic the microenvironments of specific cell lineages are also reviewed. Copyright © 2015 John Wiley & Sons, Ltd.

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Co-culture with Sertoli cells promotes proliferation and migration of umbilical cord mesenchymal stem cells

Cited by 27 publications

References 20 publications

TGF-β1 induces senescence of bone marrow mesenchymal stem cells via increase of mitochondrial ROS production

TGF-β1 induces senescence of bone marrow mesenchymal stem cells via increase of mitochondrial ROS production

Mesenchymal Stem Cells Migration Homing and Tracking

Microenvironmental factors involved in human amnion mesenchymal stem cells fate decisions

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