Co-culture with neurotrophic factor secreting cells induced from adipose-derived stem cells: Promotes neurogenic differentiation

Razavi, Shahnaz; Razavi, Mohamad Reza; Kheirollahi-Kouhestani, Mahsa; Mardani, Mohammad; Mostafavi, Firoozeh

doi:10.1016/j.bbrc.2013.09.069

Cited by 23 publications

(17 citation statements)

References 38 publications

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“…As known, NSCs and BMSCs secrete neurotrophic factors, including BDNF and NGF (17,18). The contribution of BDNF and NGF to a range of cell responses has been confirmed, including cell differentiation (19)(20)(21). Therefore, the level of BDNF and NGF in different experimental groups was detected.…”

Section: Interaction Between Nscs and Bmscs During Differentiationmentioning

confidence: 81%

Interaction between neural stem cells and bone marrow derived-mesenchymal stem cells during differentiation

et al. 2014

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Due to their capacity to self-replicate or produce specific differentiated cell types, neural stem cells (NSCs) and bone marrow derived-mesenchymal stem cells (BMSCs) are potential sources for cell transplantation therapies, particularly for neural injury. However, the interaction between NSCs and BMSCs during differentiation has not yet been defined. The interaction is believed to improve the effectiveness and efficiency of cell therapy. In the present study, human NSCs and BMSCs were cultured and the Transwell co-culture system was used to observe the interplay between NSCs and BMSCs during differentiation. The results revealed that NSCs promoted BMSCs to differentiate into neurons and NSCs; whereas, BMSCs did not affect the differentiation of NSCs. Simultaneously, co-culture increased the concentration of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF), which are secreted by NSCs and BMSCs. The present findings suggest that co-culture of NSCs and BMSCs can promote the differentiation and this process may be modulated by BDNF and NGF.

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Section: Interaction Between Nscs and Bmscs During Differentiationmentioning

confidence: 81%

Interaction between neural stem cells and bone marrow derived-mesenchymal stem cells during differentiation

et al. 2014

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“…There, they can differentiate into nerve cells and play roles in neuroprotection. Second, BMSCs increase the secretion of NGF and brain-derived neurotrophic factor (BDNF) and promote the maturation and differentiation of neurons, thereby promoting restoration of nerve damage [27], [28]. Previous studies have confirmed the therapeutic effects of BMSC transplantation in neurological disease.…”

Section: Discussionmentioning

confidence: 99%

NGF and TERT Co-Transfected BMSCs Improve the Restoration of Cognitive Impairment in Vascular Dementia Rats

2014

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Vascular dementia (VaD) is a mental disorder caused by brain damage due to cerebrovascular disease, and incidence of VaD is rising. To date, there is no known effective cure for VaD, so effort in developing an effective treatment for VaD is of great importance. The differentiation plasticity of BMSCs, in conjunction with its weak immunogenicity, makes manipulated BMSCs an attractive strategy for disease treatment. However, BMSCs often display disabled differentiation, premature aging, and unstable proliferation, reducing their neuroprotective function. These problems may be caused by the lack of telomerase activity in BMSCs. Our results show that NGF-TERT co-transfected BMSCs have a better therapeutic effect than BMSCs lacking NGF and TERT expression, demonstrated by significant improvements in learning and memory in VaD rats. The underlying mechanism might be increased expression of NGF, TrkA and SYN in the hippocampal CA1 area, which has potential implication in advancing therapeutics for VaD.

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“…ADSCs can be differentiated into NTF-SCs when cultured in NTF-SCs differentiation media. This induction can be done according to our previous method [23].…”

Section: Transdifferentiation Potential Of Adscs Into Neurotrophic Famentioning

confidence: 99%

“…ADSCs when cultured in the appropriate conditions are capable to differentiate into other cells outside their lineage such as neuronal, glial and neurotrophic factors secreting cells (NTFs) [14][15][16][19][20][21][22][23][24] (Figure 1). Thus, offers the potential to tissue maintenance and regeneration.…”

Section: Introductionmentioning

confidence: 99%

Transdifferentiation potential of adipose-derived stem cells into neural lineage and their application

Ghasemi¹,

Razavi²

2014

J Histol Histopathol

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Adipose-derived stem cells are a kind of mesenchymal stem cells which have a higher frequency in the adipose tissue and can be harvested by minimally invasive procedures. These cells are able to differentiate into other cells outside their lineage such as neuron, neurotrophic factor secreting cells and Schwann cells. Many of the identified neurotrophic factors such as brain-derived neurotrophic factor, nerve growth factor, and glial cell line-derived neurotrophic factor can be produced by adipose-derived stem cells. In addition, these cells when differentiated into neurotrophic factor secreting cells are able to secrete a significantly high level of these factors. Neurotrophic factors have a significant role in cellular processes include cell proliferation, differentiation and maturation. This article reviews the in vitro differentiation of adiposederived stem cells into neural lineage cells and clinical application.

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Co-culture with neurotrophic factor secreting cells induced from adipose-derived stem cells: Promotes neurogenic differentiation

Cited by 23 publications

References 38 publications

Interaction between neural stem cells and bone marrow derived-mesenchymal stem cells during differentiation

Interaction between neural stem cells and bone marrow derived-mesenchymal stem cells during differentiation

NGF and TERT Co-Transfected BMSCs Improve the Restoration of Cognitive Impairment in Vascular Dementia Rats

Transdifferentiation potential of adipose-derived stem cells into neural lineage and their application

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