Interaction between neural stem cells and bone marrow derived-mesenchymal stem cells during differentiation

Abstract

Due to their capacity to self-replicate or produce specific differentiated cell types, neural stem cells (NSCs) and bone marrow derived-mesenchymal stem cells (BMSCs) are potential sources for cell transplantation therapies, particularly for neural injury. However, the interaction between NSCs and BMSCs during differentiation has not yet been defined. The interaction is believed to improve the effectiveness and efficiency of cell therapy. In the present study, human NSCs and BMSCs were cultured and the Transwell co-culture system was used to observe the interplay between NSCs and BMSCs during differentiation. The results revealed that NSCs promoted BMSCs to differentiate into neurons and NSCs; whereas, BMSCs did not affect the differentiation of NSCs. Simultaneously, co-culture increased the concentration of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF), which are secreted by NSCs and BMSCs. The present findings suggest that co-culture of NSCs and BMSCs can promote the differentiation and this process may be modulated by BDNF and NGF.

Neuroprotective Potential and Paracrine Activity of Stromal Vs. Culture-Expanded hMSC Derived from Wharton Jelly under Co-Cultured with Hippocampal Organotypic Slices

“…NSCs are known to secrete growth factors like BDNF and FGF stimulating WJ-MSC differentiation into the neural direction. Our observation is coherent with Rong’s group [ 31 ], where the authors concluded that NSCs promoted BM-MSCs to differentiate into neuronal direction, the most probably via BDNF and NGF stimulatory interaction. Similar interplay was also described by Salgado [ 32 ], who underlined the importance of paracrine interactions between hMSCs and NSCs in the stimulated neurogenic niches.…”

Polydatin Protects Bone Marrow Stem Cells against Oxidative Injury: Involvement of Nrf 2/ARE Pathways

“…40 cases per million individuals are diagnosed as SCI [ 3 ]. Bone marrow-derived mesenchymal stem cells (BMSCs), which possess immunosuppressive properties and the capacity for unlimited self-amplification and for terminal differentiation [ 4 , 5 ], play a privileged role in ameliorating neuronal damage in CNS disease models including SCI [ 6 ]. Cellular replacement with MSCs in different SCI animal models has showed functional recovery [ 7 , 8 ].…”

Combined transplantation of neural stem cells and bone marrow mesenchymal stem cells promotes neuronal cell survival to alleviate brain damage after cardiac arrest via microRNA-133b incorporated in extracellular vesicles

“…A recent study has demonstrated the ability of MSCs to secrete EVs containing multiple miRNAs that can be transferred to cells, and miR-223 is highly expressed in BMSCs [ 25 ]. Notably, interaction between BMSCs and NSCs has been documented to fundamentally exercise the simulative effects on differentiation of BMSCs into NSCs and neuron cells [ 26 ]. Additionally, miR-133b has been proven to be delivered to the neurons via EVs secreted from MSCs [ 15 ].…”