Co-culture with Mature Islet Cells Augments the Differentiation of Insulin-Producing Cells from Pluripotent Stem Cells

Oh, Bea Jun; Oh, Seung-Hoon; Choi, Jin Kyeong; Jin, Sang‐Man; Shim, Wooyoung; Lee, Myung-Shik; Lee, Moon‐Kyu; Kim, Kwang-Won; Kim, Jae Hyun

doi:10.1007/s12015-014-9554-8

Cited by 6 publications

(7 citation statements)

References 39 publications

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“…F127 is a nonionic surfactant that assists penetration of AM esters into cells. A low dose of F127 [0.1–0.15% (v/v)] was safely added to islets, as well as a β-cell line 14,18 , but in these studies, the effect on fluorescence was not quantified. We showed that 0.2% F127 enhances fluorescence intensity.…”

Section: Discussionmentioning

confidence: 99%

Determination of Islet Viability Using a Zinc-Specific Fluorescent Dye and a Semiautomated Assessment Method

et al. 2016

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Islet transplantation is an effective therapy that allows the achievement of insulin independence in patients with type 1 diabetes (T1D). To ensure successful transplantation, islet viability and function are of great importance. Viability assessments most often use fluorescein diacetate (FDA)/propidium iodide (PI) staining. However, results using this method often do not correlate well with graft function. Because FDA nonspecifically penetrates all cells present in the islet preparation, including islets and contaminating acinar cells, its use often complicates viability assessments of the overall cell population. Furthermore, the manual method for determining viability percentages is highly subjective. Shortcomings of the conventional islet viability assay can be potentially improved by staining cells with Newport Green (NG). NG, is a zinc-specific fluorescent dye that specifically reacts with zinc-rich β cells. Two kinds of NG dyes, NG-DCF and NG-PDX, are currently available. We examined the zinc specificity of these NG dyes and compared NG staining with traditional FDA staining to explore the potential of NG dyes to improve islet viability assessment. Of the two NGs tested, NG-DCF showed the higher specificity toward a β-cell line as well as human islets. NG-DCF accurately identified the islet area, even in low-purity islets, while neither FDA nor NG-PDX did. Although NG-DCF staining required a longer incubation time, the addition of poloxamer F127 and incubation at 37°C allowed viability assessment to take place within 30 min. Unlike FDA/PI staining, NG-DCF/PI staining allowed for islet-specific assessment. We also introduced a semiautomated measurement to determine NG-DCF/PI staining results, which enabled us to obtain objective and reproducible results. NG-DCF/PI staining is easy and reliable, and this method permits highly objective islet-specific viability assessments.

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Section: Discussionmentioning

confidence: 99%

Determination of Islet Viability Using a Zinc-Specific Fluorescent Dye and a Semiautomated Assessment Method

et al. 2016

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“…Nowadays extensive research on the use of stem cell has shown a hope for cure of DM [21] [22]. Stem cells that have been investigated in the treatment of DM include pluripotent stem cells (PSCs), mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) [7] [21] [22].…”

Section: Discussionmentioning

confidence: 99%

“…Stem cells that have been investigated in the treatment of DM include pluripotent stem cells (PSCs), mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) [7] [21] [22].…”

Section: Discussionmentioning

confidence: 99%

Use of Human Embryonic Stem Cells in the Treatment of Diabetes Mellitus: A Case Series

Shroff¹

2015

JDM

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Introduction: Diabetes mellitus (DM), a metabolic disorder, is known to be highly prevalent in people aged 40 -60 years in developing countries whereas in developed countries, it mostly affects people above the age of 60 years. It is of two types: DM type I, an autoimmune disorder that mostly onsets after an infection and DM type II that is commonly associated with obesity. Several treatments are available for the treatment of DM, but none has successfully cured diabetes. Nowadays, stem cell therapy is being investigated for use in the treatment of DM and has shown positive results. Case Report: Our study presented results of three diabetic patients who were treated with human embryonic stem cell (hESC) therapy. Following the therapy, blood glucose levels were reduced. An improvement was observed in eye sight, stamina, gait pattern endurance, mental focus ability and muscle strength. There was a reduction in secondary side effects of high blood sugar such as affectation of cardiac, kidneys, polyneuropathy, vision etc. No adverse events and teratoma formation were observed after the treatment. Conclusion: It was concluded that hESCs showed good therapeutic potential in the treatment of patients with diabetes.

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“…Bea et al (2015) cultured pluripotent SCs in the presence of mature islet cells. They found that the paracrine effects of these cells could lead to an increase in the differentiation efficiency of the pancreatic endoderm (PE) cells into mature IPCs [28]. A study performed by Yilmaz et al (2015) revealed that the ESC coculture with mouse pancreatic islets resulted in higher differentiation efficacy toward IPCs [29].…”

Section: Coculture Methods Used For Differentiation Of Scs Intomentioning

confidence: 99%

“…They found that these cells normalized hyperglycemia in diabetic mice [35]. Another study on diabetic nude mice indicated that transplantation diabetic mice with IPCs derived from pancreatic stem cells cocultured with islet cells exhibited restored euglycemia and improved glucose tolerance [28]. Qu et al (2014) also revealed that administration of laminin 411-induced IPCs rapidly and significantly downregulated fasting blood glucose levels, significantly reduced the HbA 1 c concentration, and markedly improved the symptoms and survival of diabetic rats [32].…”

Section: Use Of In Vivo Microenvironmental Simulation Formentioning

confidence: 99%

Efficiency of Stem Cell (SC) Differentiation into Insulin-Producing Cells for Treating Diabetes: a Systematic Review

Nemati

Omrani

Ebrahimi

et al. 2021

Stem Cells International

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Over the recent years, the use of stem cells has provided a new opportunity to treat various disorders including diabetes. Stem cells are unspecialized cells with a capacity for self-renewal and differentiation into more specialized cell types. Many factors contribute to the differentiation of SCs and thus play an important role in regulating the fate of stem cells. Accordingly, a wide range of protocols has been used to differentiate SCs to insulin-producing cells but the effectiveness of SC differentiation varies. The aim of this systematic review was to evaluate the results obtained from different studies on SC differentiation for higher efficacy to treat diabetes. This search was done in PubMed, Web of Science (WOS), and Scopus using keywords “insulin-producing cell (IPC),” “pancreatic B cell,” “insulin-secreting cell,” “stem cell,” “progenitor cells,” “mother cell,” and “colony-forming unit.” Among more than 3646 papers, 32 studies were considered eligible for more evaluations. The obtained results indicated that most of the studies were performed on the mesenchymal stem cells (MSCs) derived from different tissues as compared with other types of SCs. Different evaluations of in vitro studies as well as animal models supported their role in the recovery of diabetes. In the present review, we summarize and discuss recent advances in increasing the efficiency of SC differentiation using different materials, but despite the promising results of this systematic review, further studies are needed to assess the efficiency and safety of transplantation of these cells in diabetes recovery.

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Co-culture with Mature Islet Cells Augments the Differentiation of Insulin-Producing Cells from Pluripotent Stem Cells

Cited by 6 publications

References 39 publications

Determination of Islet Viability Using a Zinc-Specific Fluorescent Dye and a Semiautomated Assessment Method

Determination of Islet Viability Using a Zinc-Specific Fluorescent Dye and a Semiautomated Assessment Method

Use of Human Embryonic Stem Cells in the Treatment of Diabetes Mellitus: A Case Series

Efficiency of Stem Cell (SC) Differentiation into Insulin-Producing Cells for Treating Diabetes: a Systematic Review

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