Co-culture with Endothelial Progenitor Cells promotes the Osteogenesis of Bone Mesenchymal Stem Cells via the VEGF-YAP axis in high-glucose environments
Abstract:Patients with type 2 diabetes mellitus (T2DM) have a high risk of fracture and experience poor bone healing. In recent years, bone mesenchymal stem cells (BMSCs) and endothelial progenitor cells (EPCs) have become the most commonly used cells in cell therapy and tissue engineering. In this study, we found that high glucose levels had a negative effect on the differentiation of BMSCs and EPCs. Considering that EPCs-BMSCs sheets can provide endothelial cells and osteoblastic cells, we transplanted cell sheets in… Show more
“…After SAPH infill, the ECs showed slightly weakened ALP activities when compared with those of the PCL scaffold. 27 In all cases, the ALP of the BMSC/EC co-culture was always higher than the average ALP of the BMSCs and ECs alone (Fig. 4F), suggesting some EC-induced BMSC osteogenic differentiation in the co-culture.…”
Segmental bone defects over the self-healing threshold are a major challenge for orthopedics. Despite advances in clinical practice, traditional tissue engineering methods are limited by the addition of heterogeneous cells...
“…After SAPH infill, the ECs showed slightly weakened ALP activities when compared with those of the PCL scaffold. 27 In all cases, the ALP of the BMSC/EC co-culture was always higher than the average ALP of the BMSCs and ECs alone (Fig. 4F), suggesting some EC-induced BMSC osteogenic differentiation in the co-culture.…”
Segmental bone defects over the self-healing threshold are a major challenge for orthopedics. Despite advances in clinical practice, traditional tissue engineering methods are limited by the addition of heterogeneous cells...
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