Co-culture with endothelial progenitor cells promotes survival, migration, and differentiation of osteoclast precursors

Pang, Haijun; Wu, Xiang; Fu, Sheng-Long; Luo, Fei; Zhang, Zehua; Hou, Tianyong; Li, Zhiqiang; Chang, Zhengqi; Yu, Bo; Xu, Jiake

doi:10.1016/j.bbrc.2012.11.081

Cited by 21 publications

(25 citation statements)

References 23 publications

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“…They have also been reported to enhance osteogenic differentiation capacities of osteoblast precursors in vitro and in vivo [17,30]. With regard to the role of EPCs in osteoclast formation and function, previous reports have shown that EPCs support the survival, migration, and differentiation of RAW 264.7 cells, a model of osteoclastic monocyte precursors [19,20]. In this study, we found that EPCs derived from mouse umbilical cord blood promoted the proliferation, migration and osteoclastic differentiation of primary mouse BMMs in a EPC-BMM coculture system.…”

Section: Discussionmentioning

confidence: 97%

“…All cells were counterstained with the carbocyanine fluorescent dye Dil (Molecular Probes, Eugene, OR, USA). The angiogenic capacity of the early EPCs was determined by the Matrigel tube formation assay as we previously reported [19]. In brief, Matrigel (Sigma) was diluted in 500 μl EGM-2 media (1:1 v/v) in 96-well plates and incubated at 37°C for 1 h to allow polymerization.…”

Section: Isolation and Characterization Of Epcsmentioning

confidence: 99%

“…Emerging evidence suggests that EPCs may also play a role in osteoclast formation and function. Specifically, EPCs have been reported to support the survival, migration, and differentiation of RAW 264.7 cells, a model of osteoclastic monocyte precursors, through multiple signaling pathways [19,20]. However, whether EPCs can influence osteoclastogenesis by bone marrow-derived macrophages (BMMs) is not clear.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Endothelial Progenitor Cells Enhance the Migration and Osteoclastic Differentiation of Bone Marrow-Derived Macrophages in vitro and in a Mouse Femur Fracture Model through Talin-1

Cui

Hou

et al. 2018

Cell Physiol Biochem

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Background/Aims: Bone resorption mediated by osteoclasts plays an important role in bone healing. Endothelial progenitor cells (EPCs) promote bone repair by stimulating neovascularization and osteogenesis. However, the role of EPCs in osteoclast formation and function is not well defined. The aim of this study was to elucidate mechanisms of EPCs in osteoclast formation and function. Methods: In this study, we examined the effects of EPCs on the proliferation, migration and osteoclastic differentiation of primary mouse bone marrow-derived macrophages (BMMs) in a co-culture system in vitro. We also evaluated the effects of EPC co-transplantation on the homing and osteoclastic differentiation of transplanted BMMs in a mouse bone fracture model in vivo. The technology of immunofluorescence, immunohistochemical, western blot, Rt-PCR, cell co-culture and Transwell were used in this study. Results: EPCs secreted TGF-β1 in the EPC-BMM co-culture medium and increased Talin-1 expression in the co-cultured BMMs. Treatment with a TGF-β1 neutralizing antibody or Talin-1 silencing in BMMs completely inhibited BMM osteoclastic differentiation in the co-culture system. These results indicated that the osteoclastogenic effects of EPCs were mediated by TGF-β1-mediated Talin-1 expression in BMMs. In the femur fracture model, BMMs co-transplanted with EPCs exhibited enhanced engraftment into the fracture site and osteoclastic differentiation compared with those transplanted alone. Mice treated with EPC-BMM co-transplantation exhibited increased neovascularization at the fracture site and accelerated fracture healing compared with those treated with BMMs alone. Conclusion: Taken together, the results suggest that EPCs can promote bone repair by enhancing recruitment and differentiation of osteoclast precursors.

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Section: Discussionmentioning

confidence: 97%

Section: Isolation and Characterization Of Epcsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Endothelial Progenitor Cells Enhance the Migration and Osteoclastic Differentiation of Bone Marrow-Derived Macrophages in vitro and in a Mouse Femur Fracture Model through Talin-1

Cui

Hou

et al. 2018

Cell Physiol Biochem

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“…In short, bone marrow cells obtained from tibia of 8-to 10-week-old female C57BL/6 mice were cultured in a-minimal essential medium (MEMa) containing 10% FBS for [16][17][18][19][20] …”

Section: Cell Culturementioning

confidence: 99%

Netrin‐4 derived from murine vascular endothelial cells inhibits osteoclast differentiation in vitro and prevents bone loss in vivo

et al. 2014

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a b s t r a c tBone is a highly vascularized organ, thus angiogenesis is a vital process during bone remodeling. However, the role of vascular systems in bone remodeling is not well recognized. Here we show that netrin-4 inhibits osteoclast differentiation in vitro and in vivo. Co-cultures of bone marrow macrophages with vascular endothelial cells markedly inhibited osteoclast differentiation. Adding a neutralizing antibody, or RNA interference against netrin-4, restored in vitro osteoclast differentiation. Administration of netrin-4 prevented bone loss in an osteoporosis mouse model by decreasing the osteoclast number. We propose that vascular endothelial cells interact with bone in suppressing bone through netrin-4.

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“…To date, various recruitment signals have been identified, notable among which is chemokine CXCL12, strongly expressed in stromal cells located in the perivascular regions of the bone marrow. The osteoclast precursors express the receptor of chemokine CXCR4, whose union with CXCL12 promotes the recruitment and survival of the OCs 15 . The CXCL12/CXCR4 axis has become a target of great interest in oncology 16,17 due to its key role in the migratory behaviour of tumour cells, although, taking into account the above, it is highly probable that it also participates in functions such as accelerated bone remodelling which occurs in postmenopausal osteoporosis, or in the different forms of bone destruction which characterise rheumatoid arthritis.…”

Section: Migration Of the Precursorsmentioning

confidence: 99%

Osteoclastos: mucho más que células remodeladoras del hueso

Arboleya

Castañeda

2014

Rev Osteoporos Metab Miner

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SummaryThe osteoclast has been considered classically as a cell with the exclusive function of bone remodelling, with a gregarious behaviour. However, advances which have been made in recent years have changed this concept drastically, and we now know that this multinuclear cell is subject to complex biological regulation, necessary for it to exert a multifunctional role of unknown dimensions. In addition to its participation as the only cell capable of reabsorbing the calcified bone matrix, the osteoclast is one of the cellular elements effective in the immune system, a function still little-known but expected, given its belonging to the monocyte-macrophage lineage. Its role in other processes, both local, such as as a collaborative element in osteoformation and hematopoietic stem cell niche maintenance, and systemic, is also beginning to be understood. In this review the most significant findings contributing to our understanding of the biology of the osteoclast are analysed, with an eminently practical content and an approach aimed at understanding the possible molecular targets which will allow a better therapeutic treatment of such important diseases as osteoporosis, arthritis or cancer.

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Co-culture with endothelial progenitor cells promotes survival, migration, and differentiation of osteoclast precursors

Cited by 21 publications

References 23 publications

Endothelial Progenitor Cells Enhance the Migration and Osteoclastic Differentiation of Bone Marrow-Derived Macrophages in vitro and in a Mouse Femur Fracture Model through Talin-1

Endothelial Progenitor Cells Enhance the Migration and Osteoclastic Differentiation of Bone Marrow-Derived Macrophages in vitro and in a Mouse Femur Fracture Model through Talin-1

Netrin‐4 derived from murine vascular endothelial cells inhibits osteoclast differentiation in vitro and prevents bone loss in vivo

Osteoclastos: mucho más que células remodeladoras del hueso

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