Co-culture of Bacillus amyloliquefaciens ACCC11060 and Trichoderma asperellum GDFS1009 enhanced pathogen-inhibition and amino acid yield

Abstract

BackgroundBacillus spp. are a genus of biocontrol bacteria widely used for antibiosis, while Trichoderma spp. are biocontrol fungi that are abundantly explored. In this study, a liquid co-cultivation of these two organisms was tried firstly.Results and discussionThrough liquid chromatography-mass spectrometry/mass spectrometry (LC–MS/MS), it was discovered that with an inoculation in the ratio of 1.9:1, the antimicrobial effect of the co-cultured fermentation liquor of Bacillus amyloliquefaciens ACCC11060 and Trichoderma asperellum GDFS1009 was found to be significantly higher than that of pure-cultivation. A raise in the synthesis of antimicrobial substances contributed to this significant increase. Additionally, a co-culture with the inoculation of the two organisms in the ratio of 1:1 was found to enhance the production of specific amino acids. This technique could be further explored for either a large scale production of amino acids or could serve as a theoretical base for the generation of certain rare amino acids.ConclusionsThis work clearly demonstrated that co-cultivation of B. amyloliquefaciens ACCC11060 and T. asperellum GDFS1009 could produce more specific biocontrol substances and amino acids.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-1004-x) contains supplementary material, which is available to authorized users.

Co-cultivation of Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841 Causes Differential Gene Expression and Improvement in the Wheat Growth and Biocontrol Activity

“…In the present investigation, the L -Proline, N -pivaloyl- ethyl ester was found in only co-culture system and this might be responsible for the highest seed germination, biocontrol and plant growth (Table 5 and Figure 7). Similarly, Wu et al (2018) observed that the proline metabolism was induced in the co-culture of B. amyloliquefaciens ACCC11060 and T. asperellum using co-inoculation method.…”

“…The beneficial effect of T. asperellum and B. amyloliquefaciens includes protection against pathogens, growth promotion, induction of disease resistance, and production of bioactive compounds with antibiotic and antitumor properties (Brunner et al, 2005; Xie et al, 2018). The previous study on the co-cultivation of B. amyloliquefaciens and T. asperellum inhibited the growth of B. cinerea (Wu et al, 2018). Hence in the present study, stimulation of diverse cellular processes such as sporulation, secondary metabolism, enzyme productions in the co-culture of T. asperellum GDFS1009 and B. amyloliquefaciens 1841 were studied to evaluate its effect on biocontrol, and plant growth.…”

“…It also leads to the synthesis of enzymes to increase the metabolite production (Abdelmohsen et al, 2015). Wu et al (2018) found that the antibiosis of B. amyloliquefaciens ACCC11060 and T. asperellum GDFS1009 was increased under co-cultivation conditions. Hence, in the present study, the optimal medium for B. amyloliquefaciens 1841 and T. asperellum GDFS1009 cultivation was developed using response surface methodology.…”

Trichoderma virens Gl006 and Bacillus velezensis Bs006: a compatible interaction controlling Fusarium wilt of cape gooseberry

“…have been conducted mainly in vitro. For instance, Wu et al 16 evaluated the antimicrobial effect of co-culturing Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens ACCC11060 in BP broth (beef extract 0.3% and peptone 0.5%) finding the greatest effects against Botrytis cinerea by the supernatant from the co-culture than individual treatments. Other study demonstrated that in vitro co-culture of T. asperellum GDFS1009 and B. amyloliquefaciens 1841 induced the production of compounds not detected under pure cultures, attributed to the competition between them 17 .…”

Microbial Interactions Within Multiple-Strain Biological Control Agents Impact Soil-Borne Plant Disease

“…In simple cases, MSBCAs consist of only two strains, e.g., a fungus and a bacterium where one or both have biocontrol activities. A consortium consisting of Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens ACCC1111060 was found to be more efficient against infection by Botrytis cinerea (the agent of gray mold disease) than the individual strains ( Wu et al, 2018 ). Similarly, when Trichoderma virens GI006 was combined with Bacillus velezensis Bs006, efficiency against Fusarium wilt of cape gooseberry was enhanced ( Izquierdo-García et al, 2020 ).…”