Co-cultivation of <i>Rhodotorula glutinis</i> and <i>Chlorella pyrenoidosa</i> to improve nutrient removal and protein content by their synergistic relationship

Li, Huankai; Zhong, Yuming; Lu, Qian; Zhang, Xin; Liu, Huifan; Diao, Zeng-Hui; Yao, Chuang; Liu, Hui

doi:10.1039/c9ra01884k

Cited by 30 publications

(17 citation statements)

References 53 publications

(57 reference statements)

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“…What seemed evident, confirming what has already been observed in previous studies [31,32,49,50,51], was the presence of a synergistic effect that in our case was attributed to different processes. During the lag phase, L. starkeyi showed a latent growth phase (Fig.…”

Section: Study Of C Saccharophilum and L Starkeyi Single And Mixed Cultures In Arundo Donax Hydrolysatesupporting

confidence: 93%

“…1 for mixed culture. The choice of a 1:1 ratio of microalga:yeast was made in relation to what already observed in a previous study [31] and according to what already highlighted, for example by Ling et al [34] and Li et al [32]. An imbalance in the microalga:yeast inoculum ratio generally motivated by their different growth rates, besides not inducing a net increase in terms of lipid productivity, can sometimes lead to an accentuation for N and P competition, and consequently to an imbalance in pH.…”

Section: Resultsmentioning

confidence: 97%

“…In addition, an increase in the microalga:yeast ratio would promote algal metabolic activity by inducing an increase in pH, that could inhibit yeast growth. On the contrary, microalgae growth would be inhibited, due to excessive nutrient consumption caused by a high yeast:microalga ratio [32]. In BBM+G, the growth of C. saccharophilum showed an initial trend correlated to the glucose consumption (Fig.…”

Section: Parameter Analysismentioning

confidence: 99%

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Biorefinery-Based Approach to Exploit Mixed Cultures of Lipomyces Starkeyi and Chloroidium saccharophilum for Single Cell Oil Production

Zuccaro¹,

Mondo²,

Pinto³

et al. 2020

Preprint

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The mutualistic interactions between the oleaginous yeast Lipomyces starkeyi and the green mi-croalga Chloroidium saccharophilum in mixed cultures were investigated to exploit possible syner-gistic effects. As a matter of facts, microalga could act as an oxygen generator for the yeast, while the yeast could provide carbon dioxide to microalga. A lignocellulosic hydrolysate from steam exploded Arundo donax (Giant reed) was used as low cost feedstock. The overall lipid content and lipid productivity obtained in the mixed culture treating the hydrolysate of Arundo donax were equal to 0.081 glipid.gbiomass-1 and 37.2 mglipid.L-1.d-1, respectively. They represented promising re-sults if compared to the model systems where synthetic media were used. This study provided new input for the integration of Single Cell Oil (SCO) production with agro-industrial feedstock and the fatty acid distribution mainly consisting of stearic (C18:0) and oleic acid (C18:1) allows promising applications in biofuels, cosmetics, food additives and other products of industrial interest.

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Section: Study Of C Saccharophilum and L Starkeyi Single And Mixed Cultures In Arundo Donax Hydrolysatesupporting

confidence: 93%

Section: Resultsmentioning

confidence: 97%

Section: Parameter Analysismentioning

confidence: 99%

See 1 more Smart Citation

Biorefinery-Based Approach to Exploit Mixed Cultures of Lipomyces Starkeyi and Chloroidium saccharophilum for Single Cell Oil Production

Zuccaro¹,

Mondo²,

Pinto³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…In fact, it changed from 0.583 g•g −1 for C. saccharophilum alone to 0.596 g•g −1 for L. starkeyi alone and 0.44 g•g −1 for mixed culture. The choice of a 1:1 ratio of microalga:yeast was made in relation to what was observed in a previous study [31] and according to what was highlighted, for example, by Ling et al [34] and Li et al [33]. An imbalance in the microalga:yeast inoculum ratio generally motivated by their different growth rates, besides not inducing a net increase in terms of lipid productivity, can sometimes lead to an accentuation for N and P competition, and consequently to an imbalance in pH.…”

Section: Samplementioning

confidence: 99%

“…In the presence of co-cultures (Figures 1c, 2c and 3c), the virtuous loop CO 2 -O 2 helped glucose consumption, but the progressive imbalance in the C/N ratio is also a form of inhibition that prevents a proportional increase in the concentration of microbial biomass. On the contrary, microalgae growth would be inhibited, due to excessive nutrient consumption caused by a high yeast:microalga ratio [33].…”

Section: Samplementioning

confidence: 99%

Biorefinery-Based Approach to Exploit Mixed Cultures of Lipomyces starkeyi and Chloroidium saccharophilum for Single Cell Oil Production

et al. 2021

View full text Add to dashboard Cite

The mutualistic interactions between the oleaginous yeast Lipomyces starkeyi and the green microalga Chloroidium saccharophilum in mixed cultures were investigated to exploit possible synergistic effects. In fact, microalga could act as an oxygen generator for the yeast, while the yeast could provide carbon dioxide to microalga. The behavior of the two microorganisms alone and in mixed culture was studied in two synthetic media (YEG and BBM+G) before moving on to a real model represented by the hydrolysate of Arundo donax, used as low-cost feedstock, and previously subjected to steam explosion and enzymatic hydrolysis. The overall lipid content and lipid productivity obtained in the mixed culture of YEG, BBM+G and for the hydrolysate of Arundo donax were equal to 0.064, 0.064 and 0.081 glipid·gbiomass−1 and 30.14, 35.56 and 37.22 mglipid·L−1·day−1, respectively. The mixed cultures, in all cases, proved to be the most performing compared to the individual ones. In addition, this study provided new input for the integration of Single Cell Oil (SCO) production with agro-industrial feedstock, and the fatty acid distribution mainly consisting of stearic (C18:0) and oleic acid (C18:1) allows promising applications in biofuels, cosmetics, food additives and other products of industrial interest.

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Recent advances and fundamentals of microalgae cultivation technology

Rozenberg,

Sorokin,

Mukhambetova

et al. 2024

Biotechnology Journal

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Microalgae are considered to be a promising group of organisms for fuel production, waste processing, pharmaceutical applications, and as a source of food components. Unicellular algae are worth being considered because of their capacity to produce comparatively large amounts of lipids, proteins, and vitamins while requiring little room for growth. They can also grow on waste and fix CO2 and nitrogen compounds. However, production costs limit the industrial use of microalgae to the most profitable applications including micronutrient production and fish farming. Therefore, novel microalgae based technologies require an increase of the production efficiencies or values. Here we review the recent studies focused on getting strains with novel characteristics or cultivating techniques that improve production's robustness or efficiency and categorize these findings according to the fundamental factors that determine microalgae growth. Improvements of light and nutrient delivery, as well as other aspects of photobioreactor design, have shown the highest average increase in productivity. Other methods, such as an improvement of phosphorus or CO2 fixation and temperature adaptation have been found to be less effective. Furthermore, interactions with particular bacteria may promote the growth of microalgae, although bacterial and grazer contaminations must be managed to avoid culture failure. The competitiveness of the algal products will increase if these discoveries are applied to industrial settings.

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Co-cultivation of Rhodotorula glutinis and Chlorella pyrenoidosa to improve nutrient removal and protein content by their synergistic relationship

Abstract: Aiming at treating piggery wastewater and producing useful products, a novel method using a co-culture of Chlorella pyrenoidosa and Rhodotorula glutinis in piggery wastewater was proposed in this study to improve nutrient removal and the protein content in the feed produced.

Cited by 30 publications

References 53 publications

Biorefinery-Based Approach to Exploit Mixed Cultures of Lipomyces Starkeyi and Chloroidium saccharophilum for Single Cell Oil Production

Biorefinery-Based Approach to Exploit Mixed Cultures of Lipomyces Starkeyi and Chloroidium saccharophilum for Single Cell Oil Production

Biorefinery-Based Approach to Exploit Mixed Cultures of Lipomyces starkeyi and Chloroidium saccharophilum for Single Cell Oil Production

Recent advances and fundamentals of microalgae cultivation technology

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