Co-acclimation of bacterial communities under stresses of hydrocarbons with different structures

Abstract

Crude oil is a complex mixture of hydrocarbons with different structures; its components vary in bioavailability and toxicity. It is important to understand how bacterial communities response to different hydrocarbons and their co-acclimation in the process of degradation. In this study, microcosms with the addition of structurally different hydrocarbons were setup to investigate the successions of bacterial communities and the interactions between different bacterial taxa. Hydrocarbons were effectively degraded in all microcosms after 40 days. High-throughput sequencing offered a great quantity of data for analyzing successions of bacterial communities. The results indicated that the bacterial communities responded dramatically different to various hydrocarbons. KEGG database and PICRUSt were applied to predict functions of individual bacterial taxa and networks were constructed to analyze co-acclimations between functional bacterial groups. Almost all functional genes catalyzing degradation of different hydrocarbons were predicted in bacterial communities. Most of bacterial taxa were believed to conduct biodegradation processes via interactions with each other. This study addressed a few investigated area of bacterial community responses to structurally different organic pollutants and their co-acclimation and interactions in the process of biodegradation. The study could provide useful information to guide the bioremediation of crude oil pollution.

Effect of digestate application on microbial respiration and bacterial communities' diversity during bioremediation of weathered petroleum hydrocarbons contaminated soils

“…In treatments amended with digestate also an increase of taxa containing recognized TPH degraders was observed. In S1 soil for treatments CDA and CDBI Mycobacterium species were still present, however new dominant genera appeared including Arenimonas which was previously observed in hydrocarbon degrading cultures and associated with oil degradation (Reyes-Sosa et al, 2018;Wang et al, 2016), Arthrobacter (for CDA only) able to degrade crude oil components (Reyes-Sosa et al, 2018) and Thermomonas which was observed in microbial communities under intensive oil degradation (Al-Kharusi et al, 2016).…”

“…This genus was not previously assigned to TPH degradation activity and surprisingly it was linked with mercury resistance (Pepi et al, 2011). Among other top genera, Acinetobacter species were shown to be able to use alkanes as a carbon and energy source and possess alkB genes (Liu et al, 2011;Nie et al, 2014;Wang et al, 2011), while Mycobacterium was previously observed to catalyse different reaction and express wide range of catabolic genes including alkB genes (Nie et al, 2014;Wang et al, 2016). In treatments amended with digestate also an increase of taxa containing recognized TPH degraders was observed.…”

Bacterial succession and co‐occurrence patterns of an enriched marine microbial community during light crude oil degradation in a batch reactor

“…Muricauda is a common heterotrophic marine bacterium, with several representative members isolated (Hwang et al ). Their contribution to the degradation of aliphatic hydrocarbons (Jiménez et al ) and pristane (Wang et al ) have been reported, as well as their preference for sub products from hydrocarbon degradation (Bruns et al ). However, their relationships or dependence with other taxa are still unknown.…”

Bacterial nanocellulose production from naphthalene

“…The biofilm architecture is structurally supported by the extracellular polymeric substances (EPS) produced by the community members, which provide a number of advantages for the community, such as stability, cell–cell proximity favouring interactions and genetic exchange (Madsen et al ., ; Ren et al ., ), protection against stressors and predators, passive sorption of solutes, amongst other benefits. In fact, in hydrocarbon‐polluted sites, environmental bacterial isolates often only degrade a narrow range of PAHs, and cooperative processes involving a consortium of strains with complementary capacities are frequently observed (Bouchez et al ., ; Wang et al ., ).…”