Effect of digestate application on microbial respiration and bacterial communities' diversity during bioremediation of weathered petroleum hydrocarbons contaminated soils

Abstract

Revised version resubmitted to Science of the Total Environment Highlights Digestate application with bulking agent or immobilized bacteria improve TPH removal Digestate effect on soil respiration depends on soil texture Sewage sludge digestate contains high concentrations of alkB genes *Manuscript (double-spaced and continuously LINE and PAGE numbered)-for final publication Click here to view linked References Highlights Digestate application with bulking agent or immobilized bacteria improve TPH removal Digestate effect on soil respiration depends on soil texture Sewage sludge digestate contains high concentrations of alkB genes Application of digestate increases and maintains alkB genes content in the soil Distinct microbial groups developed in amended and non-amended soils *Highlights (for review : 3 to 5 bullet points (maximum 85 characters including spaces per bullet point)

Hydrocarbon Removal by Two Differently Developed Microbial Inoculants and Comparing Their Actions with Biostimulation Treatment

“…Although we did not study the influence of natural attenuation on hydrocarbon removal in great details, our results show relatively low effectiveness of this approach compared to other reports [65,[76][77][78]. In turn, other authors also noticed insufficient effects of naturally occurring processes as a remediation strategy [79,80].…”

“…Natural attenuation occurs in environments, but at least one of following conditions must be fulfilled: (1) it should be the adequate concentration of available P and N, and/or (2) the soil texture should allow bacteria to have facilitated access to hydrocarbons and oxygen. In laboratory conditions, the latter could be achieved by passing the soil through a sieve [76][77][78]. However, this practice was not applied in this study.…”

Theoretical Substantiation of the Petroleum Hydrocarbons Destruction by Specific Microflora Using Anaerobic Digestate

“…Single research [38] indicated high efficiency of digestate as a potential nutrient and microbial seeding for bioremediation of petroleum hydrocarbon contaminated soils. Addition of digestate together with saw dust decreased aggregates size and improved soil respiration.…”

Dry Anaerobic Digestion for Agricultural Waste Recycling

“…The prokaryotic community structure of the treatments reported by Wang [82] was evaluated, and the results showed that Mix2 treatment, in which low NO 3 − risk and high nematode suppression were confirmed, was separated from the other treatments, indicating that a specific microbial community was developed in the treatment (Figure 2). Several papers have already reported that the application of biogas digestate affected the community structure of bacteria and fungi [122][123][124]. In general, organic amendment stimulates a broad range of (micro) organisms involved in the soil food web, many of which are potential predators, such as diplogasterid [125] and dorylaimid [126], or invertebrate antagonists, such as enchytraeids and earthworms [127].…”