CMOS On-Chip Optoelectronic Neural Interface Device with Integrated Light Source for Optogenetics

Sawadsaringkarn, Yosmongkol; Kimura, Hiroshi; Maezawa, Yasuyo; Nakajima, Arata; Kobayashi, Takuma; Sasagawa, Kiyotaka; Noda, Toshihiko; Tokuda, Takashi

doi:10.1088/1742-6596/352/1/012004

Cited by 4 publications

(3 citation statements)

References 22 publications

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“…Several works [45]–[47] attempted to incorporate optogenetic control, with or without some form of recordings. Pashaie et al designed a close-loop optogenetic brain interface based on micro-ECoG and fluorescence microscopy [45].…”

Section: Introductionmentioning

confidence: 99%

“…Paralikar et al implemented an implantable 5 mW/channel dual-wavelength optogenetic stimulator, without a neural recording element [46]. Sawadsaringkarn et al also published a CMOS optoelectronic neural interface based on an image sensor with an on-chip light stimulator [47]. However, the integration of both action-potential recording and optogenetic control in a single monolithic integrated circuit (IC) is still lacking.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An Integrated Circuit for Simultaneous Extracellular Electrophysiology Recording and Optogenetic Neural Manipulation

Chen

McCullagh

Pun

et al. 2017

IEEE Trans. Biomed. Eng.

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The ability to record and to control action potential firing in neuronal circuits of the brain is critical to understand how the brain functions on the cellular and network levels. Recent development of optogenetic proteins allows direct stimulation or inhibition of action potential firing of neurons upon optical illumination. In this paper, we combined a low-noise and high input impedance (or low input capacitance) neural recording amplifier, and a high current laser/LED driver in a monolithic integrated circuit (IC) for simultaneous neural recording and optogenetic neural control. The low input capacitance of the amplifier (9.7 pF) was achieved through adding a dedicated unity gain input stage optimized for high impedance metal electrodes. The input referred noise of the amplifier was measured to be 4.57 µVrms, which is lower than the estimated thermal noise of the metal electrode. Thus, action potentials originating from a single neuron can be recorded with a signal-to-noise ratio of ~6.6. The LED/laser current driver delivers a maximum current of 330 mA to generate adequate light for optogenetic control. We experimentally tested the functionality of the IC with an anesthetized Mongolian gerbil and recorded auditory stimulated action potentials from the inferior colliculus. Furthermore, we showed that spontaneous firing of 5th (trigeminal) nerve fibers was inhibited using the optogenetic protein Halorhodopsin. A noise model was also derived including the equivalent electronic components of the metal electrode and the high current driver to guide the design.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An Integrated Circuit for Simultaneous Extracellular Electrophysiology Recording and Optogenetic Neural Manipulation

Chen

McCullagh

Pun

et al. 2017

IEEE Trans. Biomed. Eng.

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“…Such a technique would be useful for functional brain analysis and could be used to control perception, behavior, and emotion in a freely moving animal. As such, optical neural interface devices have been investigated heretofore 26 27 28 29 30 31 . In the present study, an implantable device incorporating optogenetics and Ca 2+ imaging and its control system was newly developed.…”

mentioning

confidence: 99%

“Optical communication with brain cells by means of an implanted duplex micro-device with optogenetics and Ca2+ fluoroimaging”

Kobayashi

Haruta

Sasagawa

et al. 2016

Sci Rep

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To better understand the brain function based on neural activity, a minimally invasive analysis technology in a freely moving animal is necessary. Such technology would provide new knowledge in neuroscience and contribute to regenerative medical techniques and prosthetics care. An application that combines optogenetics for voluntarily stimulating nerves, imaging to visualize neural activity, and a wearable micro-instrument for implantation into the brain could meet the abovementioned demand. To this end, a micro-device that can be applied to the brain less invasively and a system for controlling the device has been newly developed in this study. Since the novel implantable device has dual LEDs and a CMOS image sensor, photostimulation and fluorescence imaging can be performed simultaneously. The device enables bidirectional communication with the brain by means of light. In the present study, the device was evaluated in an in vitro experiment using a new on-chip 3D neuroculture with an extracellular matrix gel and an in vivo experiment involving regenerative medical transplantation and gene delivery to the brain by using both photosensitive channel and fluorescent Ca2+ indicator. The device succeeded in activating cells locally by selective photostimulation, and the physiological Ca2+ dynamics of neural cells were visualized simultaneously by fluorescence imaging.

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Controlling Behavior Using Light to Excite and Silence Neuronal Activity

Çetin

Komai

2013

Methods in Neuroethological Research

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CMOS On-Chip Optoelectronic Neural Interface Device with Integrated Light Source for Optogenetics

Cited by 4 publications

References 22 publications

An Integrated Circuit for Simultaneous Extracellular Electrophysiology Recording and Optogenetic Neural Manipulation

An Integrated Circuit for Simultaneous Extracellular Electrophysiology Recording and Optogenetic Neural Manipulation

“Optical communication with brain cells by means of an implanted duplex micro-device with optogenetics and Ca2+ fluoroimaging”

Controlling Behavior Using Light to Excite and Silence Neuronal Activity

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