CMOS image sensor integrated with micro-LED and multielectrode arrays for the patterned photostimulation and multichannel recording of neuronal tissue

Abstract

We developed a complementary metal oxide semiconductor (CMOS) integrated device for optogenetic applications. This device can interface via neuronal tissue with three functional modalities: imaging, optical stimulation and electrical recording. The CMOS image sensor was fabricated on 0.35 μm standard CMOS process with built-in control circuits for an on-chip blue light-emitting diode (LED) array. The effective imaging area was 2.0 × 1.8 mm². The pixel array was composed of 7.5 × 7.5 μm² 3-transistor active pixel sensors (APSs). The LED array had 10 × 8 micro-LEDs measuring 192 × 225 μm². We integrated the device with a commercial multichannel recording system to make electrical recordings.

Pixel pitch and particle energy influence on the dark current distribution of neutron irradiated CMOS image sensors

“…CMOS Image Sensors (CIS) [1][2][3], also called Active Pixel Sensors (APS), have become the main detector technology for optical imaging systems [1][2][3][4][5][6] in consumer and high-end scientific applications [4][5][6]. These optical sensors are exposed to harsh radiation environments in a wide variety of imaging applications (medical imaging [4][5][6][7], space remote sensing [8,9], nuclear power plant monitoring, inertial confinement fusion plasma diagnostics [10,11]). These radiation environments contain particles (e.g.…”

Continuous sensing of tumor-targeted molecular probes with a vertical cavity surface emitting laser-based biosensor

“…An optical sensor enables continuous, long-term sensing of targeted probe dynamics in freely moving subjects at potentially high temporal resolution. Continuous optical sensing with a miniature design has been pursued with complementary metal oxide semiconductor (CMOS) detector arrays, [15][16][17] fiber optics, 18 and integrated III-V semiconductor devices. 19 We have developed a miniature semiconductor-based fluorescent sensor containing a vertical-cavity surface-emitting laser (VCSEL), gallium arsenide PIN diode, and a fluorescence emission filter.…”

Optogenetics

“…The typical stimulation intensity used in optogenetics experiments is within 10 mW/mm 2 . The core of the device is a multifunctional CMOS image sensor, which we have developed in previous works (Tokuda et al 2007a(Tokuda et al , b , 2012 Nakajima et al 2012 ;Sawadsaringkarn et al 2013 ). This comparison suggests that if we place a GaInN blue LED near the target neural cells, we can perform optical stimulation of rhodopsin proteins.…”