Cm³⁺/Eu³⁺induced structural, mechanistic and functional implications for calmodulin

Abstract

Trivalent lanthanide and actinide can strongly bind to calmodulin (CaM). The global structure of Ln/An-bound CaM were found to be similar to Ca-CaM but the local environment around Ln/An is distorted giving less structural rigidity to Ln/An-CaM.

Americium preferred: lanmodulin, a natural lanthanide-binding protein favors an actinide over lanthanides

“…The luminescence lifetimes for the Eu 3+ -and Cm 3+ -aquo ions are in good accordance to previously reported ones and the spectral characteristics of the formed Eu 3+ – and Cm 3+ –LanM species match reported species of the calcium-binding protein calmodulin. 25,26 Due to the greater sensitivity and quantum yields of Cm 3+ luminescence compared to Eu 3+ , 27 a lower concentration of 18 nM LanM and 100 nM Cm 3+ was utilized, while for Eu 3+ experiments a final concentration of 1 μM of LanM and 5.5 μM Eu 3+ was necessary. For both experiments, 5.5 equivalents of Eu 3+ or Cm 3+ were required to obtain a 100% occupation of all 4 EF hands of LanM which also corresponded with CD experiments (Fig.…”

Interaction analyses of SARS-CoV-2 spike protein based on fragment molecular orbital calculations

“…49,50 Several MD simulations were performed to investigate dynamical behaviors of the spike protein(s) as well. [51][52][53] Even though the FMO calculation for the hydrated spike protein is currently impractical to execute, a combination simulation of MD and FMO [54][55][56] for the RBD complexes may be one of our future tasks. As denoted in ESI Part 1, † detailed analyses with singular value decomposition (SVD) 57,58 as a data-science technique have been in progress for a huge amount of PIEDA data on the spike protein, especially to elucidate the importance of inter-chain charged residue pairs in structural differences.…”

High-Gradient Magnetic Separation of Compact Fluorescent Lamp Phosphors: Elucidation of the Removal Dynamics in a Rotary Permanent Magnet Separator

“…Thus, the emission spectrum, especially when combined with the luminescence decay, carries information about the phosphor and is a unique fingerprint of it. The TRLFS data were analyzed using parallel factor analysis (PARAFAC, N-way toolbox for Matlab [22]), which was previously successfully used for TRLFS data of ionic f-elements [23,24]. Here, we demonstrate the use of TRLFS in combination with PARAFAC for the quantification of an individual phosphor's concentration when suspended in a mixture with other phosphors.…”

“…A deconvolution of the phosphors' emission spectra by PARAFAC requires trilinearity of components [24]. More specifically, in the case of our TRLFS setup, this means that each PARAFAC component must have a unique distribution within the (1) sample series, (2) emission spectrum and (3) luminescence decay.…”