Americium preferred: lanmodulin, a natural lanthanide-binding protein favors an actinide over lanthanides

Abstract

The separation and recycling of lanthanides is an active area of research with a growing demand that calls for more environmentally friendly lanthanide sources. Likewise, the efficient and industrial separation...

Lanmodulin peptides – unravelling the binding of the EF-Hand loop sequences stripped from the structural corset

“…The analogously conducted experiment with Cm( iii ) again pointed out the similarity of the four different peptides: the binding affinities for Cm( iii ) are in the same range ( Table 2 ). A comparison of Cm( iii ) and Eu( iii ) luminescence lifetimes after interaction with the full protein 25 and isolated EF-Hand loop peptides further supports the current conclusions from experiment and simulation. The luminescence lifetimes were significantly shorter for the EF-Hand loop peptides compared with the full protein (180 μs vs .…”

“…The luminescence lifetimes were significantly shorter for the EF-Hand loop peptides compared with the full protein (180 μs vs . 370 μs 25 for Eu( iii ) and 140 μs vs . 200 μs 25 for Cm( iii )).…”

“…370 μs 25 for Eu( iii ) and 140 μs vs . 200 μs 25 for Cm( iii )). This indicates more water and less peptide functionalities in the first coordination sphere of the metal centre.…”

Not just a background: pH buffers do interact with lanthanide ions—a Europium(III) case study

“…However, the interpretation and decomposition of Eu(III) luminescence emission data sets is significantly complicated when Eu(III) is complexed or even present as mixed-ligand (ternary) complexes. This was the case in our previous study, investigating Eu(III) binding to lanmodulin [ 46 ] in the presence of PIPES as buffer. Consequently, we were obliged to switch to TRIS to overcome this drawback.…”

Improved Recovery and Selectivity of Lanthanide-Ion-Binding Cyclic Peptide Hosts by Changing the Position of Acidic Amino Acids

“…To date, various organic hosts for Ln 3+ [4][5][6][7][8], peptide hosts for organic guests [9][10][11], peptide-transition metal complexes [12][13][14][15][16], and self-assembling systems [17][18][19] have been reported. In recent years, extended research on Ln porphyrins [20], lanthanide-based metal organic frameworks [21], lanthanide-based inorganic-organic hybrid materials [22], a cyclic peptide ligand for Tb ions through high-throughput screening [23], and a lanthanidebinding protein [24] have also been interesting. We have also used a lanthanide-binding Minerals 2022, 12, 148 2 of 13 tag (LBT) of a linear peptide that recognizes Ln 3+ to report on the interaction mechanism between the binding affinity for each Ln 3+ and the molecular structure of the LBT peptide [25].…”