Clusters of Mycobacterium tuberculosis Genotypes in Odesa Region

Antonenko, Peter B.; Kresyun, V. I.; Antonenko, Kate O.

doi:10.15407/microbiolj78.02.103

Cited by 5 publications

(3 citation statements)

References 9 publications

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“…So, patients' genotype polymorphism has certain impact on the drugs' concentration in the blood and finally on the effectiveness of TB treatment. For example, TB patients with "slow metabolizers" (SM) genotype of CYP2C9 gene had the highest serum isoniazid and rifampicin level and the most favorable treatment outcome comparatively to "rapid metabolizers" (RM) genotype group [8]. According to CYP3A4*1B genotype in TB patients with RM genotype, the indexes of cytolysis (alanine aminotransferase, aspartate aminotransferase and bile stasis (gamma-glutathione transferase) were higher comparatively to SM genotype both before and after in-patient treatment [9].…”

Section: клінічна практикаmentioning

confidence: 99%

“…The blood samples were collected from TB patients during the first 2 weeks of in-patient treatment 2, 4, 6, and 24 h after administration of rifampicin and isoniazid. The level of rifampicin was determined according to Chubaryan method with modification that is based on the extraction of rifampicin from the blood using chloroform and KON with further spectrophotometric analysis of the extract at 470 nm [8]. The content of isoniazid was determined according to the method of Wallenberg in modification of Shenderov [8]; isoniazid forms a colored complex with vanadium-acidic ammonia in the acidic medium; the intensity of coloring can be measured at a wavelength of 400 nm.…”

Section: клінічна практикаmentioning

confidence: 99%

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Prognostic Value of Cyp3a4*1b Polymorphism in Patients With Pulmonary Tuberculosis

Poludenko,

Antonenko,

Antonenko

et al. 2024

The Odesa Medical Journal

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Introduction. Previously it was found that in slow metabolizers according to CYP3A4*1G genotype after completion of in-patient stage of anti-tuberculosis treatment, the level of cytolysis and toxicity indexes was much higher than in rapid metabolizers. The aim of the present research was to find the meaning of CYP3A4*1В polymorphism in tuberculosis (TB) patients for the level of isoniazid and rifampicin as well as for the outcome and toxicity development during in-patient TB treatment.Materials and methods. The medical records of 105 patients with pulmonary tuberculosis were examined. All these patients had primary pulmonary tuberculosis. They were receiving in-patient treatment at the Odesa Regional Center for Socially Significant Diseases (previouslythe Odesa Regional Tuberculosis Dispensary) in 2012-2014. The study was conducted under the Declaration of Helsinki standards.Results. After in-patient treatment, the activity of cytolysis markers as alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and the cholestasis marker as gamma-glutathione transferase (GGT) in TB-patients with *AA genotype increased insignificantly by 7.0%, 9.3%, and 4.5% (p>0.05); in patients with the *AG genotype, the activity of ALT, AST and GGT, on the contrary, had a tendency for decreasing -by 18.7%, 3.0% and 9.0% (p>0.05); a similar trend was observed concerning the number of patients with increased activity of ALT, AST and GGT. At the end of treatment, the average activity of ALT in carriers of the *AA genotype was 1.8 times higher than in carriers of the *AG genotype (p=0.046; . Conclusion.At the beginning of anti-tuberculosis chemotherapy, it is recommended to determine the CYP3A4*1B genotype in patients with pulmonary tuberculosis, which allow identifying the groups of patients with the *AG genotype, which is characterized by a greater risk of developing sub-therapeutic rifampicin concentrations in the blood during treatment and prove the usefulness of personalized choice of rifampicin dosage according to the CYP3A4*1B genotype.

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Section: клінічна практикаmentioning

confidence: 99%

Section: клінічна практикаmentioning

confidence: 99%

Prognostic Value of Cyp3a4*1b Polymorphism in Patients With Pulmonary Tuberculosis

Poludenko,

Antonenko,

Antonenko

et al. 2024

The Odesa Medical Journal

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“…The peculiarity of the TB incidence in Ukraine is the intensive spreading of the Mycobacterium tuberculosis strains of the Beijing family, associated with MDR, from 39.6% in 2003 to 54.8% in 2012. 3 One of the reasons for the development of MDR of M. tuberculosis could be the insufficient (under-effective) serum concentration of anti-TB drugs. According to Chideya et al, the low serum concentrations of isoniazid and rifampicin were observed in 37% and 84% of TB patients, respectively; 4 these numbers according to Um et al were 15% and 24%, respectively.…”

Section: Introductionmentioning

confidence: 99%

Serum isoniazid concentration in the patients as an indicator of the effectiveness and toxicity of tuberculosis treatment

Antonenko

Kresyun

et al. 2019

Pol. Ann. Med.

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Introduction The low serum concentration of the anti-tuberculosis (TB) drugs in TB patients is observed quite often, but the significance of this phenomenon remains controversial. Aim The aim of the presented research was to clarify the association between isoniazid concentration and TB-treatment outcomes. Material and methods Blood samples were obtained from 86 patients with newly diagnosed pulmonary TB at Odessa Regional TB Hospital in 2015. The level of isoniazid in blood was measured 2 h, 4 h, 6 h, and 24 h after the administration of isoniazid. The medical records of the enrolled patients at the beginning and at the end of the in-patient treatment, and the activity of the biochemical indices were considered. Results and discussion At the end of the in-patient treatment in patients with a serum isoniazid concentration of more than 2 µg/mL (HIC), 4 h after the drug administration, the resorption of TB infiltrates in lungs occurred 1.3 times more frequently than in patients with an isoniazid concentration of less than 2 µg/mL (LIC). According to the culture method, the smear conversion occurred 3.1 times more often in patients with HIC than in the LIC ones. A positive correlation was found between the isoniazid serum level with the bilirubin level, thymol probe, and the AsAT activity, which proved the higher risk of hepatotoxicity in patients with HIC. Conclusions Thus, the measurement of the isoniazid concentration after 4 h of isoniazid administration can predict the outcome of the TB-treatment. It is postulated that the recommended concentration of isoniazid in blood 4 h after its administration > 2 µg/mL.

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The Effect of Immunoglobulin G on the Humoral Immunity in Patients with Tuberculosis/HIV Coinfection

Matsegora,

Kaprosh,

Vasylyeva

et al. 2024

AIDS Research and Human Retroviruses

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Clusters of Mycobacterium tuberculosis Genotypes in Odesa Region

Cited by 5 publications

References 9 publications

Prognostic Value of Cyp3a4*1b Polymorphism in Patients With Pulmonary Tuberculosis

Prognostic Value of Cyp3a4*1b Polymorphism in Patients With Pulmonary Tuberculosis

Serum isoniazid concentration in the patients as an indicator of the effectiveness and toxicity of tuberculosis treatment

The Effect of Immunoglobulin G on the Humoral Immunity in Patients with Tuberculosis/HIV Coinfection

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