The present study was conducted to assess the genetic diversity among maize accessions indigenous to Pakistan. The research was directed in the field area of plant breeding and genetics, University of Agriculture Faisalabad. The experiment was laid down in a randomized complete block design with three replications. The characters that are studied were Days to 50% tasseling, Days to 50% silking, Anthesis silking interval, Plant height (cm), Ear height (cm), Cob length (cm), Cob diameter (mm), No of grains per cob, 100-grain weight (g), Cob yield per plant (g), Grain yield per plant (g). At the maturity, stage data were collected for all the traits. Analysis of variance revealed that all the genotypes under study were highly significant for all the traits. The principal component analysis (PCA) showed that the first four principal component analysis displayed eigenvalues with >1 and imparted 83.44% share to total variability. According to scatter plot genotypes UAF-PB-805, UAF-PB-378, UAF-PB-794, UAF-PB-364, and UAF-PB-871 were genetically diverse and should be considered for selection program. Dendrogram classified twenty genotypes into nine clusters based on their similarity. Cluster 9 had maximum intra-cluster distance depicting more variability as compared to other clusters. The genotypes in these clusters had high variability for study attributes. These results showed that the inbred lines having widely divergent clusters can be utilized in the hybrid breeding program.