Clustered genes involved in cyclopiazonic acid production are next to the aflatoxin biosynthesis gene cluster in Aspergillus flavus

Chang, Perng‐Kuang; Horn, Bruce W.; Dorner, Joe W.

doi:10.1016/j.fgb.2008.11.002

Cited by 128 publications

(118 citation statements)

References 59 publications

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“…3) The cpaA, cpaD, and cpaO genes are required for CPA biosynthesis. [6][7][8] We previously demonstrated that cpaH is involved in the modification of CPA to 2-oxoCPA. 4) The other genes, cpaR, cpaM, and cpaT, are predicted to encode a C6-type transcription factor, methyltransferase, and major facilitator superfamily (MFS) transporter protein, 4) respectively, but have not yet been characterized because gene disruption did not affect CPA production in A. oryzae.…”

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confidence: 99%

Cyclopiazonic acid biosynthesis gene cluster gene cpaM is required for speradine A biosynthesis

Tokuoka

Kikuchi

Shinohara

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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confidence: 99%

Cyclopiazonic acid biosynthesis gene cluster gene cpaM is required for speradine A biosynthesis

Tokuoka

Kikuchi

Shinohara

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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“…These results are in accordance with the previous result that disruption of cpaR did not affect CPA production in A. flavus. 4) Thus, another trans-element, one other than cpaR, might regulate the cpa cluster. LaeA, which is considered a global transcriptional regulator of secondary metabolite gene clusters in Aspergillus fungi, 5) is thought to be involved in CPA production in A. flavus.…”

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confidence: 99%

Functional Analysis of the Cyclopiazonic Acid Biosynthesis Gene Cluster inAspergillus oryzaeRIB 40

Shinohara

Tokuoka

Koyama

2011

Bioscience, Biotechnology, and Biochemistry

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The cyclopiazonic acid (CPA) nonproducing strain, Aspergillus oryzae RIB 40, does not biosynthesize cycloacetoacetyl-L-tryptophan (cAATrp) due to a truncation in the responsible PKS-NRPS gene. We found that RIB 40 converted cAATrp to 2-oxocyclopiazonic acid, the final product of CPA biosynthesis in A. oryzae. This indicates that the CPA biosynthesis gene cluster, except for the PKS-NRPS gene, is functional in RIB 40.

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“…lavus may also produce CPA, which is toxic in a variety of animals and has been implicated in human poisoning. CPA and alatoxins commonly occur together in contaminated agricultural commodities [35].…”

Section: Resultsmentioning

confidence: 99%

First Report of Mycotoxins in Second Peanuts Crop in Adana and Osmaniye at Harvest, Drying, Prestorage and Storage Periods

Lavkor¹,

Var²,

Öztemiz³

et al. 2017

Aflatoxin-Control, Analysis, Detection and Health Risks

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Alatoxin (AF) and cyclopiazonic acid (CPA) contaminations are very important problems for peanuts and its products. The aim of the study was to detect alatoxin (types B and G) and cyclopiazonic acid (CPA) occurrence and critical periods of toxin production in peanuts collected from diferent research areas of Osmaniye and Adana, Turkey, in 2015. Peanut kernels toxin analysis was performed in four diferent periods during the harvest, drying, prestorage, and storage. Total alatoxin occurrence in peanut kernels was analyzed by immunoainity chromatography-reversed-phase high-performance liquid chromatography (IAC-HPLC) analysis and cyclopiazonic acid occurrence in peanut kernels was analyzed by thin layer chromatography (TLC). Alatoxin levels in 76 out of 102 contaminated samples were from 0.3 to 1333.42 μg/kg. Cyclopiazonic acid levels in 18 out of 102 peanut samples were from 16.6 to 44.44 μg/kg. An unusual patern of mycotoxin production (alatoxin types B and G simultaneously with CPA) was seen in 11 of 102 peanuts samples. Six of nine samples were from the storage period. Alatoxin contamination during harvesting (64%) and drying (75%) were higher than prestorage (53%). Alatoxin (93%) and cyclopiazonic acid (30%) were the most produced during storage. The results showed that storage period was signiicantly important for the presence of two mycotoxins according to the statistical analysis.

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Clustered genes involved in cyclopiazonic acid production are next to the aflatoxin biosynthesis gene cluster in Aspergillus flavus

Cited by 128 publications

References 59 publications

Cyclopiazonic acid biosynthesis gene cluster gene cpaM is required for speradine A biosynthesis

Cyclopiazonic acid biosynthesis gene cluster gene cpaM is required for speradine A biosynthesis

Functional Analysis of the Cyclopiazonic Acid Biosynthesis Gene Cluster inAspergillus oryzaeRIB 40

First Report of Mycotoxins in Second Peanuts Crop in Adana and Osmaniye at Harvest, Drying, Prestorage and Storage Periods

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