Cluster of clinical isolates of epidemic methicillin-resistant Staphylococcus aureus (EMRSA) with a negative deoxyribonuclease (DNase) test–implications for laboratory diagnosis and infection control

Rao, J.; Qamruddin, A.; Hassan, Ibrahim A.; Burnie, James; Ganner, M.

doi:10.1053/jhin.2002.1225

Cited by 12 publications

(11 citation statements)

References 3 publications

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“…It should be noted that rare strains of S. aureus might be coagulase-negative. In addition, 5% (6/119) of the isolates were negative by the DNase test, which was also reported by Rao et al (2002) and Kateete et al (2010). Other biochemical tests included mannitol semi-solid medium, semi-solid glucose medium, maltose plus purple agar base, urease, catalase, and oxidase.…”

Section: Bacterial Identificationsupporting

confidence: 56%

In vitro antimicrobial activity of the organic extract of Cladonia substellata Vainio and usnic acid against Staphylococcus spp. obtained from cats and dogs

et al. 2017

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It contains several secondary metabolites with biological activity, including usnic acid, which has exhibited a wide range of biological activities. The aim of this study was to evaluate the in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid. Initially, Staphylococcus spp., derived from samples of skin and ears of dogs and cats with suspected pyoderma and otitis, were isolated and analyzed. In antimicrobial susceptibility testing against Staphylococcus spp., 77% (105/136) of the isolates were resistant to the antimicrobials tested. In the assessment of biofilm production, 83% (113/136) were classified as producing biofilm. In genetic characterization, 32% (44/136) were positive for blaZ, no isolate (0/136) was positive for the mecA gene, and 2% (3/136) were positive for the icaD gene. The in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid against Staphylococcus spp. ranged from 0.25mg/mL to 0.0019mg/mL, inhibiting bacterial growth at low concentrations. The substances were more effective against biofilm-producing bacteria (0.65mg/mL-0.42mg/mL) when compared to non-biofilm producing bacteria (2.52mg/mL-2.71mg/mL). Usnic acid and the organic extract of C. substellata can be effective in the treatment of pyoderma and otitis in dogs and cats caused by Staphylococcus spp.

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Section: Bacterial Identificationsupporting

confidence: 56%

In vitro antimicrobial activity of the organic extract of Cladonia substellata Vainio and usnic acid against Staphylococcus spp. obtained from cats and dogs

et al. 2017

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“…The objective of the DNAse test in this study was to distinguish S. aureus from CoNS species. However, this test should be used with caution since it may identify CoNS isolates as S. aureus or S. aureus isolates as CoNS as reported in other studies (Rao et al, 2002;Bello and Qahtani, 2005;Kateete et al, 2010).…”

Section: Discussionmentioning

confidence: 82%

“…Rao et al (2002) detected four MRSA isolates with a negative DNAse result that were confirmed to be S. aureus. However, in the present study no DNAse-negative isolate was identified as S. aureus.…”

Section: Discussionmentioning

confidence: 99%

Identification of Staphylococcus saprophyticus isolated from patients with urinary tract infection using a simple set of biochemical tests correlating with 16S–23S interspace region molecular weight patterns

Ferreira

Bonesso

Mondelli

et al. 2012

Journal of Microbiological Methods

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The emergence of Staphylococcus spp. not only as human pathogens, but also as reservoirs of antibiotic resistance determinants, requires the development of methods for their rapid and reliable identification in medically important samples. The aim of this study was to compare three phenotypic methods for the identification of Staphylococcus spp. isolated from patients with urinary tract infection using the PCR of the 16S-23S interspace region generating molecular weight patterns (ITR-PCR) as reference. All 57 S. saprophyticus studied were correctly identified using only the novobiocin disk. A rate of agreement of 98.0% was obtained for the simplified battery of biochemical tests in relation to ITR-PCR, whereas the Vitek I system and novobiocin disk showed 81.2% and 89.1% agreement, respectively. No other novobiocin-resistant non-S. saprophyticus strain was identified. Thus, the novobiocin disk is a feasible alternative for the identification of S. saprophyticus in urine samples in laboratories with limited resources. ITR-PCR and the simplified battery of biochemical tests were more reliable than the commercial systems currently available. This study confirms that automated systems are still unable to correctly differentiate CoNS species and that simple, reliable and inexpensive methods can be used for routine identification.

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“…Over time, vancomycin emerged as the only drug to be reliably effective against MRSA infections. 7, 8 Zvethkova et al present in vitro susceptibility data on 2366 MRSA isolates collected over a fiveyear period. 1 The susceptibilities are interesting from an in vitro standpoint, but are clinically unreliable because in vitro susceptibility data against MRSA do not, per se, define or predict clinical efficacy in the treatment of MRSA infections.…”

Section: Oral Antibiotic Treatment Of Mrsa Infectionsmentioning

confidence: 99%

Oral antibiotic treatment of MRSA infections

Cunha

2005

Journal of Hospital Infection

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Cluster of clinical isolates of epidemic methicillin-resistant Staphylococcus aureus (EMRSA) with a negative deoxyribonuclease (DNase) test–implications for laboratory diagnosis and infection control

Cited by 12 publications

References 3 publications

In vitro antimicrobial activity of the organic extract of Cladonia substellata Vainio and usnic acid against Staphylococcus spp. obtained from cats and dogs

In vitro antimicrobial activity of the organic extract of Cladonia substellata Vainio and usnic acid against Staphylococcus spp. obtained from cats and dogs

Identification of Staphylococcus saprophyticus isolated from patients with urinary tract infection using a simple set of biochemical tests correlating with 16S–23S interspace region molecular weight patterns

Oral antibiotic treatment of MRSA infections

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