Clozapine recognition via molecularly imprinted polymers; bulk polymerization versus precipitation method

Abstract

Two clozapine (CLZ) imprinted polymers were prepared by bulk and precipitation methods. Methacrylic acid and ethylene glycol dimethacrylate (EDMA) were used as functional and crosslinker monomers, respectively. The mean diameter and particle size distribution of the imprinted (P-MIP) and nonimprinted (P-NIP) particles obtained in precipitation method were examined. A conventional batch-adsorption test was applied for characterization of CLZ-polymer interaction. Dissociation constant (K D ) and maximum binding sites (B max ) were calculated using Scatchard analysis. To evaluate the recognition properties of polymers, phenytoin (PTN) binding to each polymer was also studied and compared to CLZ. The imprinting factor (IF) and selectivity factor (a) were also determined for each polymer. Average diameter and polydispersity of P-MIP were 925 nm and 0.17, respectively. The data for P-NIP were 1.05 lm and 0.18. The K D , IF, and a values calculated for P-MIP were 0.45 lM, 3.26, and 17.43, respectively. The data for imprinted polymer, prepared by bulk polymerization (B-MIP), were 14.5 lM, 1.95, and 3.67. These results demonstrated that precipitation polymerization is a more convenient, more effective, and more reproducible method than bulk polymerization for the synthesis of uniformly sized micron and submicronimprinted polymer particles.

Application of molecularly imprinted hydrogel for the preparation of lactose‐free milk

“…The binding properties of hydrogels were studied in a conventional batch rebinding test . Briefly, dry hydrogel (10 mg) was incubated, in 2 mL distilled water, with lactose and was shaken for 2 h at room temperature.…”

“…The preparation of specific binding sites via the molecular imprinting technique has become the main objective of many studies and major interest of some researchers in recent years . Molecularly imprinted polymers (MIPs) are synthesised by radical polymerisation of monomers in the presence of a suitable template molecule.…”

Preparation of Molecularly Imprinted Polymer Based on Combinatorial Imprinted for Recognition of Norfloxacin

“…Study of the Coordination Behavior Between NFA, Zn (II) and MAA by UV Spectrophotometry The specific recognition ability of CMIP to template molecule depends on the type of inter-molecular force, number of binding sites and combining stability of functional monomers with template functional groups [18] . So, MAA was chosen as the functional monomer to combine with NFA in this research.…”

Synthesis of Uniform and Stable Molecularly Imprinted Polymer Particles by Precipitation Polymerization

“…Therefore, non-covalent imprinting has currently turned into the most famous and useful synthesis for MIPs preparation. Several polymerization processes such as homogeneous (bulk polymerization 11 ) and heterogeneous systems such as suspension 12 , miniemulsion 13 , seeded emulsion 14 , emulsion 15 and precipitation 5,9,10,16,17 polymerizations are used to prepare MIPs. Bulk polymerization is firstly used to prepare MIPs.…”