A high-performance liquid chromatographic method was developed for the analysis of capsaicinoid compounds, the pungent principles of capsicum fruits. A sequential simplex method was applied to optimize the chromatographic response function used to assess the quality of separation by varying the chromatographic parameters. The separation was achieved in 11 min using a C-8 column of 15-cm length and 4.6 mm diameter using a UV detector. A flow rate of 1.15 ml min -1 at a column temperature of 43.5˚C using 63.7% methanol in water gave the most efficient separation. The method was found to be suitable for the determination of the major capsaicinoid compounds in the capsicum samples.
In this work, precipitation polymerization was used to prepare uniform and stable molecular imprinting polymers (MIPs) particles without post treatment. Melamine was used as a template molecule model, while methacrylic acid, divinyl benzene (DVB) or ethylene glycol dimethacrylate (EGDMA) and acetonitrile were used as functional monomer, cross-linker and continuous phase, respectively. The polymerization was carried out in a vacuum condition at 70°C using benzoyl peroxide as an initiator. The obtained MIP particles demonstrated spherical shape with narrow particle size distribution. The particle size varied from micrometer-sized to sub-micrometer-sized depending on the initiator content. After purification, MIP particles represented high colloidal stability in aqueous medium.The binding efficiency of MIP using both cross-linkers (DVB and EGDMA) with melamine was approximately 20 mg/g MIP. The miscibility of all components where the functional monomer effectively pre-bound with the template molecules was the main factor for obtaining MIP particle with high binding efficiency.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.