Clotrimazole-loaded N-(2-hydroxy)-propyl-3-trimethylammonium, O-palmitoyl chitosan nanoparticles for topical treatment of vulvovaginal candidiasis

Facchinatto, William Marcondes; Galante, Joana; Mesquita, Letícia; Silva, Daniella Souza; Santos, Danilo Martins dos; Moraes, Tiago Bueno; Filho, Sérgio Paulo Campana; Colnago, Luiz Alberto; Sarmento, Bruno

doi:10.1016/j.actbio.2021.02.029

Cited by 27 publications

(20 citation statements)

References 58 publications

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“…Seven papers that investigated different quaternized chitosan nanoparticles and their cytotoxicity were identified, four with in vivo studies ( Liu et al, 2015 ; Jin et al, 2017 ; Zhao et al, 2017 ; Yan et al, 2020 ), two with both ex vivo and in vitro studies ( Amidi et al, 2006 ; Yin et al, 2009 ) and one in vitro study using three different cell lines ( Facchinatto et al, 2021 ). An overview of cytotoxicity studies concerning nanoparticles with quaternized chitosan is seen in Table 6 .…”

Section: Results Of Cytotoxicity Measurements On Chitosan Containing ...mentioning

confidence: 99%

“…Only two studies showed somewhat reduced cell viability after incubation with chitosan nanoparticles ( Dehghan et al, 2013 ; Klemetsrud et al, 2018 ). Several of the papers demonstrated that chitosan in nanoparticle form was less cytotoxic than chitosan in free form ( Amidi et al, 2006 ; Yin et al, 2009 ; Pistone et al, 2017b ; Facchinatto et al, 2021 ). The active ingredient (clotrimazole and hydrochlorothiazide) also showed less cytotoxicity when incorporated in chitosan nanoparticles ( Onnainty et al, 2016 ; Facchinatto et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

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The Potential of Chitosan in Nanomedicine: An Overview of the Cytotoxicity of Chitosan Based Nanoparticles

et al. 2022

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The unique properties and applications of nanotechnology in targeting drug delivery, cosmetics, fabrics, water treatment and food packaging have received increased focus the last two decades. The application of nanoparticles in medicine is rapidly evolving, requiring careful investigation of toxicity before clinical use. Chitosan, a derivative of the natural polysaccharide chitin, has become increasingly relevant in modern medicine because of its unique properties as a nanoparticle. Chitosan is already widely used as a food additive and in food packaging, bandages and wound dressings. Thus, with an increasing application worldwide, cytotoxicity assessment of nanoparticles prepared from chitosan is of great interest. The purpose of this review is to provide an updated status of cytotoxicity studies scrutinizing the safety of chitosan nanoparticles used in biomedical research. A search in Ovid Medline from 23 March 1998 to 4 January 2022, with the combination of the search words Chitosan or chitosan, nanoparticle or nano particle or nanosphere or nanocapsule or nano capsule, toxicology or toxic or cytotoxic and mucosa or mucous membrane resulted in a total of 88 articles. After reviewing all the articles, those involving non-organic nanoparticles and cytotoxicity assays conducted exclusively on nanoparticles with anti-tumor effect (i.e., having cytotoxic effect) were excluded, resulting in 70 articles. Overall, the chitosan nanoparticles included in this review seem to express low cytotoxicity regardless of particle composition or cytotoxicity assay and cell line used for testing. Nonetheless, all new chitosan derivatives and compositions are recommended to undergo careful characterization and cytotoxicity assessment before being implemented on the market.

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Section: Results Of Cytotoxicity Measurements On Chitosan Containing ...mentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Potential of Chitosan in Nanomedicine: An Overview of the Cytotoxicity of Chitosan Based Nanoparticles

et al. 2022

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“…Clotrimazole Decreased antifungal activity in vitro but improved safety profile for CNP-associated clotrimazole [285] Callophycin A Synergetic and improved antifungal effect both in vitro and in vivo by Callophycin A in CNPs [286] Thiosemicarbazide CNP-associated thiosemicarbazide obtained a prominent reduction of fungal burden in vivo [287] Miconazole CNPs containing a seven-fold lower miconazole concentration than conventional miconazole cream obtained equal therapeutic effect [288] Miconazole and farnesol…”

Section: Candidiasismentioning

confidence: 99%

“…Chitosan nanoparticles (CNPs) were also tested for vaginal administration. Recent work by Facchinatto and colleagues explored CNPs containing clotrimazole, a widely used antifungal drug, for the localized treatment of vulvovaginal candidiasis [285]. Cationic N-(2-hydroxy)-propyl-3-trimethylammonium, O-palmitoyl CNPs were evaluated for anticandidal activity.…”

Section: Particles and Carriersmentioning

confidence: 99%

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The Expanded Role of Chitosan in Localized Antimicrobial Therapy

2021

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Chitosan is one of the most studied natural origin polymers for biomedical applications. This review focuses on the potential of chitosan in localized antimicrobial therapy to address the challenges of current rising antimicrobial resistance. Due to its mucoadhesiveness, chitosan offers the opportunity to prolong the formulation residence time at mucosal sites; its wound healing properties open possibilities to utilize chitosan as wound dressings with multitargeted activities and more. We provide an unbiased overview of the state-of-the-art chitosan-based delivery systems categorized by the administration site, addressing the site-related challenges and evaluating the representative formulations. Specifically, we offer an in-depth analysis of the current challenges of the chitosan-based novel delivery systems for skin and vaginal infections, including its formulations optimizations and limitations. A brief overview of chitosan’s potential in treating ocular, buccal and dental, and nasal infections is included. We close the review with remarks on toxicity issues and remaining challenges and perspectives.

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Dermatan Sulfate/Chitosan Nanoparticles Loaded with an Anti‐Inflammatory Peptide Increase the Response of Human Colorectal Cancer Cells to 5‐Fluorouracil

Blachman,

Birocco,

Curcio

et al. 2023

Macromolecular Bioscience

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The gold standard drug for colorectal cancer (CRC) treatment, 5‐Fluorouracil (5‐FU), induces pharmacological tolerance in long‐term management. The transcriptional factor nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NFκB) plays a key role in 5‐FU resistance. The aim of this work is to study the capability of polyelectrolytes complex nanoparticles of dermatan sulfate (DS) and chitosan (CS), loaded with the anti‐inflammatory tripeptide IRW, to sensitize colorectal cancer cells to 5‐FU. Fluorescence and flow cytometry studies confirmed the recognition by the nanoformulation, of the cluster of differentiation 44 (CD44) receptor, involved in the initiation and progression of colorectal tumors. Dynamic light scattering (DLS) and flow cytometry reinforced the importance of DS and CD44 receptor in the interaction, as the addition of DS or anti‐CD44 antibody blocked the binding. Moreover, the nanoformulation also interacts with 3D colon cancer cultures, namely colonospheres, enriched in cancer stem cells (CSC), subpopulation responsible for drug resistance and metastasis. To evaluate the consequences of this interaction, the subcellular distribution of the transcriptional factor NFκB, is determined by immunofluorescence analysis. Internalization and the intracellular release of IRW inhibited nuclear translocation of NFκB and increased cellular sensitivity to 5‐FU. Altogether, the nanoformulation could provide a selective delivery platform for IRW distribution to colorectal tumors, being an innovative strategy toward overcoming 5‐FU resistance in CRC therapy.

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Clotrimazole-loaded N-(2-hydroxy)-propyl-3-trimethylammonium, O-palmitoyl chitosan nanoparticles for topical treatment of vulvovaginal candidiasis

Cited by 27 publications

References 58 publications

The Potential of Chitosan in Nanomedicine: An Overview of the Cytotoxicity of Chitosan Based Nanoparticles

The Potential of Chitosan in Nanomedicine: An Overview of the Cytotoxicity of Chitosan Based Nanoparticles

The Expanded Role of Chitosan in Localized Antimicrobial Therapy

Dermatan Sulfate/Chitosan Nanoparticles Loaded with an Anti‐Inflammatory Peptide Increase the Response of Human Colorectal Cancer Cells to 5‐Fluorouracil

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