Clostridium perfringens type A netF and netE positive and Clostridium difficile co-infection in two adult dogs

Diniz, Amanda Nádia; Silva, Rodrigo; Oliveira, Carlos Augusto; Pierezan, Felipe; Lobato, Francisco Carlos Faria

doi:10.1016/j.anaerobe.2015.12.013

Cited by 10 publications

(5 citation statements)

References 18 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This sample was obtained from an adult dog, aged two years old. So far, C. perfringens netE þ strains were exclusively recovered from diarrheic adult dogs elsewhere [8,14], which corroborates our findings. In addition, the stool sample of this dog was positive for CPE in EIA.…”

Section: Perfringens In Cpv-positive Dogssupporting

confidence: 90%

“…Thus, although these methods could suggest that C. perfringens is involved, they are not confirmatory [30]. Recently, studies have shown that two poreforming toxins (NetE, NetF) present in C. perfringens type A strains are strongly associated with canine hemorrhagic gastroenteritis [8,14], suggesting these toxin-encoding genes could be used as virulence markers to diagnose CPAD in dogs.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Clostridium perfringens and C. difficile in parvovirus-positive dogs

et al. 2017

Self Cite

View full text Add to dashboard Cite

The aim of this study was to investigate Clostridium difficile and Clostridium perfringens in 82 diarrheic dogs positive for canine parvovirus type 2 (CPV). Enterotoxigenic C. perfringens type A was isolated from three (3.6%) dogs. One (1.2%) strain was also positive for NetE- and NetF-encoding genes, which are commonly associated with diarrhea in dogs. Toxigenic C. difficile was isolated from one animal (1.2%), which was also positive for A/B toxins. The present study identified C. difficile and C. perfringens infection in CPV-positive dogs. Further studies are necessary to clarify if clostridial infections may predispose or potentiate CPV-infection in dogs or vice versa.

show abstract

Section: Perfringens In Cpv-positive Dogssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Clostridium perfringens and C. difficile in parvovirus-positive dogs

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the present study, toxigenic C. difficile and netF-positive C. perfringens were detected together in three animals ( Table 2). These are the first confirmed coinfections of these strains in dogs and two of these cases have been described in more details in a case report [26]. Among other enteropathogens, Giardia sp.…”

mentioning

confidence: 62%

The incidence of Clostridioides difficile and Clostridium perfringens netF -positive strains in diarrheic dogs

et al. 2018

Self Cite

View full text Add to dashboard Cite

The aim of this study was to examine the incidence of Clostridioides (previously Clostridium) difficile and Clostridium perfringens in the feces of diarrheic and non-diarrheic dogs. Also, the presence of other common canine enteropathogens was examined. Toxigenic C. difficile and C. perfringens positive for the NetF-encoding gene (netF) were detected in 11 (11.9%) and seven (7.6%) diarrheic dogs, respectively. Three dogs were diagnosed simultaneously with toxigenic C. difficile and netF-positive C. perfringens. Among other enteropathogens, Giardia sp. was the most common agent detected in dogs positive for toxigenic C. difficile or netF-positive C. perfringens. The results suggest that C. difficile and C. perfringens occur more frequently as a primary cause of diarrhea.

show abstract

“…Our results suggest that C. perfringens was the driver of enteritis in the DZ birds, while in the CZA birds, either C. colinum , or C. perfringens , or both acting synergistically, could have been driving the infections or creating a dysbiotic environment that allowed the other to thrive. Synergistic C. perfringens / C. colinum co-infections have been reported previously in poultry [ 78 ], while other clostridial co-infections ( C. perfringens / C. difficile ) have been reported in humans, foals, and dogs [ 79 – 81 ]. Phylogenetically related bacterial taxa can share functional traits and fill similar metabolic niches [ 82 ]; thus, it is feasible that an environment permissive to one type of clostridia may also be permissive to another clostridia.…”

Section: Discussionmentioning

confidence: 99%

Gut microbiota and age shape susceptibility to clostridial enteritis in lorikeets under human care

et al. 2022

View full text Add to dashboard Cite

Background Enteritis is a common cause of morbidity and mortality in lorikeets that can be challenging to diagnose and treat. In this study, we examine gut microbiota in two lorikeet flocks with enteritis (Columbus Zoo and Aquarium—CZA; Denver Zoo—DZ). Since 2012, the CZA flock has experienced repeated outbreaks of enteritis despite extensive diet, husbandry, and clinical modifications. In 2018, both CZA and DZ observed a spike in enteritis. Recent research has revealed that the gut microbiota can influence susceptibility to enteropathogens. We hypothesized that a dysbiosis, or alteration in the gut microbial community, was making some lorikeets more susceptible to enteritis, and our goal was to characterize this dysbiosis and determine the features that predicted susceptibility. Results We employed 16S rRNA sequencing to characterize the cloacal microbiota in lorikeets (CZA n = 67, DZ n = 24) over time. We compared the microbiota of healthy lorikeets, to lorikeets with enteritis, and lorikeets susceptible to enteritis, with “susceptible” being defined as healthy birds that subsequently developed enteritis. Based on sequencing data, culture, and toxin gene detection in intestinal contents, we identified Clostridium perfringens type A (CZA and DZ) and C. colinum (CZA only) at increased relative abundances in birds with enteritis. Histopathology and immunohistochemistry further identified the presence of gram-positive bacilli and C. perfringens, respectively, in the necrotizing intestinal lesions. Finally, using Random Forests and LASSO models, we identified several features (young age and the presence of Rhodococcus fascians and Pseudomonas umsongensis) associated with susceptibility to clostridial enteritis. Conclusions We identified C. perfringens type A and C. colinum associated with lorikeet necrohemorrhagic enteritis at CZA and DZ. Susceptibility testing of isolates lead to an updated clinical treatment plan which ultimately resolved the outbreaks at both institutions. This work provides a foundation for understanding gut microbiota features that are permissive to clostridial colonization and host factors (e.g. age, prior infection) that shape responses to infection.

show abstract

Clostridium perfringens type A netF and netE positive and Clostridium difficile co-infection in two adult dogs

Cited by 10 publications

References 18 publications

Clostridium perfringens and C. difficile in parvovirus-positive dogs

Clostridium perfringens and C. difficile in parvovirus-positive dogs

The incidence of Clostridioides difficile and Clostridium perfringens netF -positive strains in diarrheic dogs

Gut microbiota and age shape susceptibility to clostridial enteritis in lorikeets under human care

Contact Info

Product

Resources

About